Controlled release of plasmid DNA from photo-cross-linked pluronic hydrogels

Ki Woo Chun; Jun Bae Lee; Sun Hwa Kim; Tae Gwan Park

doi:10.1016/j.biomaterials.2004.07.055

Controlled release of plasmid DNA from photo-cross-linked pluronic hydrogels

Ki Woo Chun, Jun Bae Lee, Sun Hwa Kim, Tae Gwan Park

Research output: Contribution to journal › Article › peer-review

141 Scopus citations

Abstract

Chemically cross-linked hydrogels composed of Pluronic ^TM, water-soluble tri-block copolymers of poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide), were synthesized by a photo-polymerization method to achieve controlled DNA release. Pluronic F127 was di-acrylated to form a macromer and cross-linked to form a hydrogel structure in the presence and absence of vinyl group-modified hyaluronic acid (HA). UV irradiation time and the presence of the vinyl group-modified HA affected the mechanical property of Pluronic hydrogels to a great extent. Swelling ratio, degradation, and rheological behaviors of Pluronic hydrogels were investigated. When plasmid DNA was loaded in the hydrogels for sustained delivery, various release profiles were attained by varying UV irradiation time and modified HA amounts. Entrapped DNA was gradually damaged with increasing the UV exposure time as evidenced by decreasing the transfection efficiency. The DNA fractions released from the HA/Pluronic hydrogels, however, exhibited considerable transfection efficiencies commensurate with the UV exposure time, suggesting that they were not chemically degraded during the release period and substantially maintained functional gene expression activities despite the UV irradiation.

Original language	English
Pages (from-to)	3319-3326
Number of pages	8
Journal	Biomaterials
Volume	26
Issue number	16
DOIs	https://doi.org/10.1016/j.biomaterials.2004.07.055
State	Published - Jun 2005
Externally published	Yes

Funding

This study was supported by the Korea Science and Engineering Foundation, Korea (# R01-2003-000-10362-0) and the Center for Advanced Functional Polymers, KAIST.

Funders	Funder number
Korea Science and Engineering Foundation	R01-2003-000-10362-0
KAIST

Keywords

Hydrogels
Photo-polymerization
Pluronic
Sustained gene delivery

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10.1016/j.biomaterials.2004.07.055

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title = "Controlled release of plasmid DNA from photo-cross-linked pluronic hydrogels",

abstract = "Chemically cross-linked hydrogels composed of Pluronic TM, water-soluble tri-block copolymers of poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide), were synthesized by a photo-polymerization method to achieve controlled DNA release. Pluronic F127 was di-acrylated to form a macromer and cross-linked to form a hydrogel structure in the presence and absence of vinyl group-modified hyaluronic acid (HA). UV irradiation time and the presence of the vinyl group-modified HA affected the mechanical property of Pluronic hydrogels to a great extent. Swelling ratio, degradation, and rheological behaviors of Pluronic hydrogels were investigated. When plasmid DNA was loaded in the hydrogels for sustained delivery, various release profiles were attained by varying UV irradiation time and modified HA amounts. Entrapped DNA was gradually damaged with increasing the UV exposure time as evidenced by decreasing the transfection efficiency. The DNA fractions released from the HA/Pluronic hydrogels, however, exhibited considerable transfection efficiencies commensurate with the UV exposure time, suggesting that they were not chemically degraded during the release period and substantially maintained functional gene expression activities despite the UV irradiation.",

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author = "Chun, {Ki Woo} and Lee, {Jun Bae} and Kim, {Sun Hwa} and Park, {Tae Gwan}",

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AU - Chun, Ki Woo

AU - Lee, Jun Bae

AU - Kim, Sun Hwa

AU - Park, Tae Gwan

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N2 - Chemically cross-linked hydrogels composed of Pluronic TM, water-soluble tri-block copolymers of poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide), were synthesized by a photo-polymerization method to achieve controlled DNA release. Pluronic F127 was di-acrylated to form a macromer and cross-linked to form a hydrogel structure in the presence and absence of vinyl group-modified hyaluronic acid (HA). UV irradiation time and the presence of the vinyl group-modified HA affected the mechanical property of Pluronic hydrogels to a great extent. Swelling ratio, degradation, and rheological behaviors of Pluronic hydrogels were investigated. When plasmid DNA was loaded in the hydrogels for sustained delivery, various release profiles were attained by varying UV irradiation time and modified HA amounts. Entrapped DNA was gradually damaged with increasing the UV exposure time as evidenced by decreasing the transfection efficiency. The DNA fractions released from the HA/Pluronic hydrogels, however, exhibited considerable transfection efficiencies commensurate with the UV exposure time, suggesting that they were not chemically degraded during the release period and substantially maintained functional gene expression activities despite the UV irradiation.

AB - Chemically cross-linked hydrogels composed of Pluronic TM, water-soluble tri-block copolymers of poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide), were synthesized by a photo-polymerization method to achieve controlled DNA release. Pluronic F127 was di-acrylated to form a macromer and cross-linked to form a hydrogel structure in the presence and absence of vinyl group-modified hyaluronic acid (HA). UV irradiation time and the presence of the vinyl group-modified HA affected the mechanical property of Pluronic hydrogels to a great extent. Swelling ratio, degradation, and rheological behaviors of Pluronic hydrogels were investigated. When plasmid DNA was loaded in the hydrogels for sustained delivery, various release profiles were attained by varying UV irradiation time and modified HA amounts. Entrapped DNA was gradually damaged with increasing the UV exposure time as evidenced by decreasing the transfection efficiency. The DNA fractions released from the HA/Pluronic hydrogels, however, exhibited considerable transfection efficiencies commensurate with the UV exposure time, suggesting that they were not chemically degraded during the release period and substantially maintained functional gene expression activities despite the UV irradiation.

KW - Hydrogels

KW - Photo-polymerization

KW - Pluronic

KW - Sustained gene delivery

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Controlled release of plasmid DNA from photo-cross-linked pluronic hydrogels

Abstract

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Keywords

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