Controlled incorporation of deuterium into bacterial cellulose

Junhong He; Sai Venkatesh Pingali; Shishir P.S. Chundawat; Angela Pack; A. Daniel Jones; Paul Langan; Brian H. Davison; Volker Urban; Barbara Evans; Hugh O'Neill

doi:10.1007/s10570-013-0067-4

Controlled incorporation of deuterium into bacterial cellulose

Junhong He, Sai Venkatesh Pingali, Shishir P.S. Chundawat, Angela Pack, A. Daniel Jones, Paul Langan, Brian H. Davison, Volker Urban, Barbara Evans, Hugh O'Neill

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Isotopic enrichment has been widely used for investigating the structural and dynamic properties of biomacromolecules to provide information that cannot be carried out with molecules composed of natural abundance isotopes. A media formulation for controlled incorporation of deuterium in bacterial cellulose synthesized by Gluconacetobacter xylinus subsp. sucrofermentans is reported. The purified cellulose was characterized using Fourier Transform Infra-Red spectrophotometry and mass spectrometry which revealed that the level of deuterium incorporation in the perdeuterated cellulose was greater than 90 %. Small-angle neutron scattering analysis demonstrated that the overall structure of the cellulose was unaffected by the substitution of deuterium for hydrogen. In addition, by varying the amount of D-glycerol in the media it was possible to vary the scattering length density of the deuterated cellulose. A large disk model was used to fit the curves of bacterial cellulose grown using 0 and 100 % D-Glycerol yielding a lower bound to the disk radii, R _min = 1,132 ± 6 and 1,154 ± 3 Å and disk thickness, T = 128 ± 1 and 83 ± 1 Å for the protiated and deuterated forms of the bacterial cellulose, respectively. This agrees well with the scanning electron microscopy analysis which revealed stacked sheets in the cellulose pellicles. Controlled incorporation of deuterium into cellulose will enable new types of experiments using techniques such as neutron scattering to reveal information about the structure and dynamics of cellulose and its interactions with proteins and other (bio) polymers.

Original language	English
Pages (from-to)	927-936
Number of pages	10
Journal	Cellulose
Volume	21
Issue number	2
DOIs	https://doi.org/10.1007/s10570-013-0067-4
State	Published - Apr 2014

Funding

Acknowledgments This research is funded by the Genomic Science Program, Office of Biological and Environmental Research, U.S. Department of Energy, under FWP ERKP752. The Center for Structural Molecular Biology (Project ERKP291) and the Bio-SANS beam line is supported by the Office of Biological and Environmental Research U.S. Department of Energy. Research at the Spallation Neutron Source and High Flux Isotope Reactor at Oak Ridge National Laboratory (ORNL) are supported by the Scientific User Facilities Division, Office of Basic Energy Sciences, U.S. Department of Energy (DOE). ORNL is managed by UT Battelle, LLC, for the U.S. DOE under Contract No. DE-AC05-00OR22725. SPSC acknowledges support from the DOE Great Lakes Bioenergy Research Center (DE-FC02-07ER64494) and Prof. Bruce Dale from Michigan State University. We appreciate support from the Michigan State University Mass Spectrometry and Metabolomics Core for LC–MS method development and execution. ADJ acknowledges support from Michigan AgBioResearch.

Funders	Funder number
Center for Structural Molecular Biology	ERKP291
Michigan AgBioResearch
Office of Biological and Environmental Research U.S. Department of Energy
Scientific User Facilities Division
U.S. Department of Energy	FWP ERKP752
Basic Energy Sciences
Biological and Environmental Research
Oak Ridge National Laboratory
Michigan State University
Great Lakes Bioenergy Research Center	DE-FC02-07ER64494
UT-Battelle	DE-AC05-00OR22725

Keywords

Cellulose
Deuteration
Labeling
Mass spectrometry
Small-angle neutron scattering

Access to Document

10.1007/s10570-013-0067-4

Cite this

@article{73740f4122ce49cc81f768df76d4a7fc,

title = "Controlled incorporation of deuterium into bacterial cellulose",

abstract = "Isotopic enrichment has been widely used for investigating the structural and dynamic properties of biomacromolecules to provide information that cannot be carried out with molecules composed of natural abundance isotopes. A media formulation for controlled incorporation of deuterium in bacterial cellulose synthesized by Gluconacetobacter xylinus subsp. sucrofermentans is reported. The purified cellulose was characterized using Fourier Transform Infra-Red spectrophotometry and mass spectrometry which revealed that the level of deuterium incorporation in the perdeuterated cellulose was greater than 90 %. Small-angle neutron scattering analysis demonstrated that the overall structure of the cellulose was unaffected by the substitution of deuterium for hydrogen. In addition, by varying the amount of D-glycerol in the media it was possible to vary the scattering length density of the deuterated cellulose. A large disk model was used to fit the curves of bacterial cellulose grown using 0 and 100 % D-Glycerol yielding a lower bound to the disk radii, R min = 1,132 ± 6 and 1,154 ± 3 {\AA} and disk thickness, T = 128 ± 1 and 83 ± 1 {\AA} for the protiated and deuterated forms of the bacterial cellulose, respectively. This agrees well with the scanning electron microscopy analysis which revealed stacked sheets in the cellulose pellicles. Controlled incorporation of deuterium into cellulose will enable new types of experiments using techniques such as neutron scattering to reveal information about the structure and dynamics of cellulose and its interactions with proteins and other (bio) polymers.",

keywords = "Cellulose, Deuteration, Labeling, Mass spectrometry, Small-angle neutron scattering",

author = "Junhong He and Pingali, {Sai Venkatesh} and Chundawat, {Shishir P.S.} and Angela Pack and Jones, {A. Daniel} and Paul Langan and Davison, {Brian H.} and Volker Urban and Barbara Evans and Hugh O'Neill",

year = "2014",

month = apr,

doi = "10.1007/s10570-013-0067-4",

language = "English",

volume = "21",

pages = "927--936",

journal = "Cellulose",

issn = "0969-0239",

publisher = "Springer",

number = "2",

}

TY - JOUR

T1 - Controlled incorporation of deuterium into bacterial cellulose

AU - He, Junhong

AU - Pingali, Sai Venkatesh

AU - Chundawat, Shishir P.S.

AU - Pack, Angela

AU - Jones, A. Daniel

AU - Langan, Paul

AU - Davison, Brian H.

AU - Urban, Volker

AU - Evans, Barbara

AU - O'Neill, Hugh

PY - 2014/4

Y1 - 2014/4

N2 - Isotopic enrichment has been widely used for investigating the structural and dynamic properties of biomacromolecules to provide information that cannot be carried out with molecules composed of natural abundance isotopes. A media formulation for controlled incorporation of deuterium in bacterial cellulose synthesized by Gluconacetobacter xylinus subsp. sucrofermentans is reported. The purified cellulose was characterized using Fourier Transform Infra-Red spectrophotometry and mass spectrometry which revealed that the level of deuterium incorporation in the perdeuterated cellulose was greater than 90 %. Small-angle neutron scattering analysis demonstrated that the overall structure of the cellulose was unaffected by the substitution of deuterium for hydrogen. In addition, by varying the amount of D-glycerol in the media it was possible to vary the scattering length density of the deuterated cellulose. A large disk model was used to fit the curves of bacterial cellulose grown using 0 and 100 % D-Glycerol yielding a lower bound to the disk radii, R min = 1,132 ± 6 and 1,154 ± 3 Å and disk thickness, T = 128 ± 1 and 83 ± 1 Å for the protiated and deuterated forms of the bacterial cellulose, respectively. This agrees well with the scanning electron microscopy analysis which revealed stacked sheets in the cellulose pellicles. Controlled incorporation of deuterium into cellulose will enable new types of experiments using techniques such as neutron scattering to reveal information about the structure and dynamics of cellulose and its interactions with proteins and other (bio) polymers.

AB - Isotopic enrichment has been widely used for investigating the structural and dynamic properties of biomacromolecules to provide information that cannot be carried out with molecules composed of natural abundance isotopes. A media formulation for controlled incorporation of deuterium in bacterial cellulose synthesized by Gluconacetobacter xylinus subsp. sucrofermentans is reported. The purified cellulose was characterized using Fourier Transform Infra-Red spectrophotometry and mass spectrometry which revealed that the level of deuterium incorporation in the perdeuterated cellulose was greater than 90 %. Small-angle neutron scattering analysis demonstrated that the overall structure of the cellulose was unaffected by the substitution of deuterium for hydrogen. In addition, by varying the amount of D-glycerol in the media it was possible to vary the scattering length density of the deuterated cellulose. A large disk model was used to fit the curves of bacterial cellulose grown using 0 and 100 % D-Glycerol yielding a lower bound to the disk radii, R min = 1,132 ± 6 and 1,154 ± 3 Å and disk thickness, T = 128 ± 1 and 83 ± 1 Å for the protiated and deuterated forms of the bacterial cellulose, respectively. This agrees well with the scanning electron microscopy analysis which revealed stacked sheets in the cellulose pellicles. Controlled incorporation of deuterium into cellulose will enable new types of experiments using techniques such as neutron scattering to reveal information about the structure and dynamics of cellulose and its interactions with proteins and other (bio) polymers.

KW - Cellulose

KW - Deuteration

KW - Labeling

KW - Mass spectrometry

KW - Small-angle neutron scattering

UR - http://www.scopus.com/inward/record.url?scp=84897844719&partnerID=8YFLogxK

U2 - 10.1007/s10570-013-0067-4

DO - 10.1007/s10570-013-0067-4

M3 - Article

AN - SCOPUS:84897844719

SN - 0969-0239

VL - 21

SP - 927

EP - 936

JO - Cellulose

JF - Cellulose

IS - 2

ER -

Controlled incorporation of deuterium into bacterial cellulose

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this