Controlled gene-eluting metal stent fabricated by bio-inspired surface modification with hyaluronic acid and deposition of DNA/PEI polyplexes

Taek Gyoung Kim, Yuhan Lee, Tae Gwan Park

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

A metal stent that could elute plasmid DNA (pDNA) in a controlled manner for substrate-mediated gene transfection was fabricated by first coating with hyaluronic acid (HA) and subsequent deposition of pDNA. To create robust HA coating layer on stainless steel (SS316L) surface, HA was derivatized with dopamine which is a well-known adsorptive molecule involving mussel adhesion process. The HA-coated surface was verified by various analytical techniques and proved to be very hydrophilic and stable, also showing superior biocompatibility in terms of suppressed plasma protein adsorption. For surface loading of pDNA, cationic pDNA/polyethylenimine (PEI) polyplexes were prepared and ionically adsorbed onto the HA-coated SS316L surface. The adsorbed surface exhibited evenly distributed nano-granular topography while the polyplexes maintained the nano-particular morphology. The pDNA was released out in a controlled manner for a period of 10 days with maintaining structural integrity. The dual coated substrate with HA and pDNA/PEI polyplexes exhibited greatly enhanced gene transfection efficiency, when compared to both bare substrate adsorbed with the polyplexes and PEI/pDNA polyelectrolyte multilayers. Dually functionalized stent with HA and pDNA exhibited effective biocompatibility and gene transfection.

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalInternational Journal of Pharmaceutics
Volume384
Issue number1-2
DOIs
StatePublished - Jan 15 2010
Externally publishedYes

Funding

This study was supported by the grants from the National Research Laboratory program of the Ministry of Education, Science and Technology, and the Korea Research Foundation (KRF-2006-005-J04602), and the Polymer Technology Institute, Sungkyunkwan University, Republic of Korea.

Keywords

  • Dopamine
  • Drug eluting stent
  • Gene delivery
  • Hyaluronic acid
  • Transfection

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