Abstract
A metal stent that could elute plasmid DNA (pDNA) in a controlled manner for substrate-mediated gene transfection was fabricated by first coating with hyaluronic acid (HA) and subsequent deposition of pDNA. To create robust HA coating layer on stainless steel (SS316L) surface, HA was derivatized with dopamine which is a well-known adsorptive molecule involving mussel adhesion process. The HA-coated surface was verified by various analytical techniques and proved to be very hydrophilic and stable, also showing superior biocompatibility in terms of suppressed plasma protein adsorption. For surface loading of pDNA, cationic pDNA/polyethylenimine (PEI) polyplexes were prepared and ionically adsorbed onto the HA-coated SS316L surface. The adsorbed surface exhibited evenly distributed nano-granular topography while the polyplexes maintained the nano-particular morphology. The pDNA was released out in a controlled manner for a period of 10 days with maintaining structural integrity. The dual coated substrate with HA and pDNA/PEI polyplexes exhibited greatly enhanced gene transfection efficiency, when compared to both bare substrate adsorbed with the polyplexes and PEI/pDNA polyelectrolyte multilayers. Dually functionalized stent with HA and pDNA exhibited effective biocompatibility and gene transfection.
Original language | English |
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Pages (from-to) | 181-188 |
Number of pages | 8 |
Journal | International Journal of Pharmaceutics |
Volume | 384 |
Issue number | 1-2 |
DOIs | |
State | Published - Jan 15 2010 |
Externally published | Yes |
Funding
This study was supported by the grants from the National Research Laboratory program of the Ministry of Education, Science and Technology, and the Korea Research Foundation (KRF-2006-005-J04602), and the Polymer Technology Institute, Sungkyunkwan University, Republic of Korea.
Keywords
- Dopamine
- Drug eluting stent
- Gene delivery
- Hyaluronic acid
- Transfection