Constant helical pitch of the gramicidin channel in phospholipid bilayers

J. Katsaras; R. S. Prosser; R. H. Stinson; J. H. Davis

doi:10.1016/S0006-3495(92)81888-X

Constant helical pitch of the gramicidin channel in phospholipid bilayers

J. Katsaras, R. S. Prosser, R. H. Stinson, J. H. Davis

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

X-ray diffraction has been applied in measuring the helical pitch of the gramicidin channel in oriented bilayers of dilauroylphosphatidylcholine (DLPC) and dimyristoylphosphatidylcholine (DMPC) at a polypeptide concentration of 9.1 mol %. The diffraction data show the helical pitch of gramicidin to be 4.7 +/- 0.2 A in both gel and liquid-crystalline phase bilayers, with and without monovalent cations. In addition, the width of the reflection due to the pitch of the helical gramicidin channel is consistent with a five turn helix.

Original language	English
Pages (from-to)	827-830
Number of pages	4
Journal	Biophysical Journal
Volume	61
Issue number	3
DOIs	https://doi.org/10.1016/S0006-3495(92)81888-X
State	Published - 1992
Externally published	Yes

Funding

The authors would like to thank Professors Barry M. Millman and Martha G. Shepherd for useful discussions. This research was supported by a grant from the National Sciences and Engineering Research Council of Canada.

Funders	Funder number
Natural Sciences and Engineering Research Council of Canada

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10.1016/S0006-3495(92)81888-X

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abstract = "X-ray diffraction has been applied in measuring the helical pitch of the gramicidin channel in oriented bilayers of dilauroylphosphatidylcholine (DLPC) and dimyristoylphosphatidylcholine (DMPC) at a polypeptide concentration of 9.1 mol %. The diffraction data show the helical pitch of gramicidin to be 4.7 +/- 0.2 A in both gel and liquid-crystalline phase bilayers, with and without monovalent cations. In addition, the width of the reflection due to the pitch of the helical gramicidin channel is consistent with a five turn helix.",

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AU - Katsaras, J.

AU - Prosser, R. S.

AU - Stinson, R. H.

AU - Davis, J. H.

PY - 1992

Y1 - 1992

N2 - X-ray diffraction has been applied in measuring the helical pitch of the gramicidin channel in oriented bilayers of dilauroylphosphatidylcholine (DLPC) and dimyristoylphosphatidylcholine (DMPC) at a polypeptide concentration of 9.1 mol %. The diffraction data show the helical pitch of gramicidin to be 4.7 +/- 0.2 A in both gel and liquid-crystalline phase bilayers, with and without monovalent cations. In addition, the width of the reflection due to the pitch of the helical gramicidin channel is consistent with a five turn helix.

AB - X-ray diffraction has been applied in measuring the helical pitch of the gramicidin channel in oriented bilayers of dilauroylphosphatidylcholine (DLPC) and dimyristoylphosphatidylcholine (DMPC) at a polypeptide concentration of 9.1 mol %. The diffraction data show the helical pitch of gramicidin to be 4.7 +/- 0.2 A in both gel and liquid-crystalline phase bilayers, with and without monovalent cations. In addition, the width of the reflection due to the pitch of the helical gramicidin channel is consistent with a five turn helix.

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JF - Biophysical Journal

IS - 3

ER -

Constant helical pitch of the gramicidin channel in phospholipid bilayers

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