TY - JOUR
T1 - Comprehensive examination of mesophases formed by DMPC and DHPC mixtures
AU - Harroun, Thad A.
AU - Koslowsky, Martin
AU - Nieh, Mu Ping
AU - De Lannoy, Charles François
AU - Raghunathan, V. A.
AU - Katsaras, John
PY - 2005/6/7
Y1 - 2005/6/7
N2 - Mixtures of long- and short-chain phospholipids, specifically 14:0 and 6:0 phosphatidylcholines (DMPC and DHPC), have been used successfully in NMR studies as magnetically alignable substrates for membrane-associated proteins. However, recent publications have shown that the phase behavior of these mixtures is much more complex than originally thought. Using polarized light microscopy and Small-angle neutron scattering, phase diagrams of DMPC/DHPC mixtures at molar ratios of 2, 3.2, and 5 have been determined. Generally, at temperatures below the main-chain melting transition of DMPC (TM = 23°C), an isotropic phase of disklike micelles is found. At high temperatures (T > 50°C), a lamellar phase Consisting of either multilamellar vesicles (MLV) or extended lamellae is formed, which at low lipid concentrations (e.g., MLV) coexists with an excess of water. At intermediate temperatures and lipid concentrations, a chiral nematic phase made up of wormlike micelles was observed.
AB - Mixtures of long- and short-chain phospholipids, specifically 14:0 and 6:0 phosphatidylcholines (DMPC and DHPC), have been used successfully in NMR studies as magnetically alignable substrates for membrane-associated proteins. However, recent publications have shown that the phase behavior of these mixtures is much more complex than originally thought. Using polarized light microscopy and Small-angle neutron scattering, phase diagrams of DMPC/DHPC mixtures at molar ratios of 2, 3.2, and 5 have been determined. Generally, at temperatures below the main-chain melting transition of DMPC (TM = 23°C), an isotropic phase of disklike micelles is found. At high temperatures (T > 50°C), a lamellar phase Consisting of either multilamellar vesicles (MLV) or extended lamellae is formed, which at low lipid concentrations (e.g., MLV) coexists with an excess of water. At intermediate temperatures and lipid concentrations, a chiral nematic phase made up of wormlike micelles was observed.
UR - http://www.scopus.com/inward/record.url?scp=20744436366&partnerID=8YFLogxK
U2 - 10.1021/la050018t
DO - 10.1021/la050018t
M3 - Article
AN - SCOPUS:20744436366
SN - 0743-7463
VL - 21
SP - 5356
EP - 5361
JO - Langmuir
JF - Langmuir
IS - 12
ER -