TY - JOUR
T1 - Comparative study of plant responses to carbon-based nanomaterials with different morphologies
AU - Lahiani, Mohamed H.
AU - Dervishi, Enkeleda
AU - Ivanov, Ilia
AU - Chen, Jihua
AU - Khodakovskaya, Mariya
N1 - Publisher Copyright:
© 2016 IOP Publishing Ltd.
PY - 2016/5/19
Y1 - 2016/5/19
N2 - The relationship between the morphology of carbon-based nanomaterials (CBNs) and the specific response of plants exposed to CBNs has not been studied systematically. Here, we prove that CBNs with different morphologies can activate cell growth, germination, and plant growth. A tobacco cell culture growth was found to increase by 22%-46% when CBNs such as helical multi-wall carbon nanotubes (MWCNTs), few-layered graphene, long MWCNTs, and short MWCNTs were added to the growth medium at a concentration of 50 μg ml-1. The germination of exposed tomato seeds, as well as the growth of exposed tomato seedlings, were significantly enhanced by the addition of all tested CBNs. The presence of CBNs inside exposed seeds was confirmed by transmission electron microscopy and Raman spectroscopy. The effects of helical MWCNTs on gene expression in tomato seeds and seedlings were investigated by microarray technology and real time-PCR. Helical MWCNTs affected a number of genes involved in cellular and metabolic processes and response to stress factors. It was shown that the expression of the tomato water channel gene in tomato seeds exposed to helical MWCNTs was upregulated. These established findings demonstrate that CBNs with different morphologies can cause the same biological effects and share similar mechanisms in planta.
AB - The relationship between the morphology of carbon-based nanomaterials (CBNs) and the specific response of plants exposed to CBNs has not been studied systematically. Here, we prove that CBNs with different morphologies can activate cell growth, germination, and plant growth. A tobacco cell culture growth was found to increase by 22%-46% when CBNs such as helical multi-wall carbon nanotubes (MWCNTs), few-layered graphene, long MWCNTs, and short MWCNTs were added to the growth medium at a concentration of 50 μg ml-1. The germination of exposed tomato seeds, as well as the growth of exposed tomato seedlings, were significantly enhanced by the addition of all tested CBNs. The presence of CBNs inside exposed seeds was confirmed by transmission electron microscopy and Raman spectroscopy. The effects of helical MWCNTs on gene expression in tomato seeds and seedlings were investigated by microarray technology and real time-PCR. Helical MWCNTs affected a number of genes involved in cellular and metabolic processes and response to stress factors. It was shown that the expression of the tomato water channel gene in tomato seeds exposed to helical MWCNTs was upregulated. These established findings demonstrate that CBNs with different morphologies can cause the same biological effects and share similar mechanisms in planta.
KW - aquaporin gene expression
KW - carbon-based nanomaterials
KW - germination
KW - properties of carbon nanomaterials
KW - tomato seeds
KW - uptake of carbon nanotubes
UR - http://www.scopus.com/inward/record.url?scp=84976351945&partnerID=8YFLogxK
U2 - 10.1088/0957-4484/27/26/265102
DO - 10.1088/0957-4484/27/26/265102
M3 - Article
AN - SCOPUS:84976351945
SN - 0957-4484
VL - 27
JO - Nanotechnology
JF - Nanotechnology
IS - 26
M1 - 265102
ER -