Chryseobacterium populi sp. nov., isolated from Populus deltoides endosphere

Victoria L. Bortniak; Dale A. Pelletier; Jeffrey D. Newman

doi:10.1099/ijsem.0.003140

Chryseobacterium populi sp. nov., isolated from Populus deltoides endosphere

Victoria L. Bortniak, Dale A. Pelletier, Jeffrey D. Newman

Integrative Microbiomics

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

As part of a study investigating the rhizosphere and endosphere of the Eastern cottonwood tree, Populus deltoides, a number of isolates were subjected to genome sequencing. The genome-derived 16S rRNA gene sequence of strain CF314 ^T was 97.0% similar to those of the Chryseobacterium daecheongense and Chryseobacterium polytrichastri type strains, but was essentially equidistant from many other Chryseobacterium type strains. Overall genome similarity metrics (average nucleotide identity, digital DNA-DNA hybridization, average amino acid identity) revealed greatest similarity to the Chryseobacterium daecheongense, Chryseobacterium piperi and Chryseobacterium soldanellicola type strains, but were well below the species thresholds. Strain CF314 ^T had a typical fatty acid composition for Chryseobacterium species and produced flexirubin pigments, but not carotenoids. The genome encodes a number of proteins such as a C-type lectin and terpene synthases that are also found in other plant-associated Bacteroidetes. Based on phenotypic and genomic characteristics of the strain, we propose the new species Chryseobacterium populi. The type strain is CF314 ^T =KCTC 62722 ^T =LMG 30786 ^T .

Original language	English
Article number	003140
Pages (from-to)	356-362
Number of pages	7
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	69
Issue number	2
DOIs	https://doi.org/10.1099/ijsem.0.003140
State	Published - Feb 2019

Funding

Research by J. D. N. was supported by a Lycoming College Professional Development Grant, NSF Grants 0960114 and 1248096, and a Fulbright Grant from the US State Department. D. A. P. is supported by the Genomic Science Program, US Department of Energy, Office of Science, Biological and Environmental Research, as part of the Plant Microbe Interfaces Scientific Focus Area at Oak Ridge National Laboratory (http://www.pmi.ornl.gov). Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the US Department of Energy under contract DEAC05-00OR22725. The Venn Diagram Generator and AAI Calculator used in this work were developed by Thomas Sontag, Andrew Gale, Jordan Krebs and Eileen Peluso. We are grateful to Devin Frantz, Kyle Jacobs and Emily Bohlin for preparing and submitting the strain to culture collections. Research by J. D. N. was supported by a Lycoming College Professional Development Grant, NSF Grants 0960114 and 1248096, and a Fulbright Grant from the US State Department. D. A. P. is supported by the Genomic Science Program, US Department of Energy, Office of Science, Biological and Environmental Research, as part of the Plant Microbe Interfaces Scientific Focus Area at Oak Ridge National Laboratory (http://www.pmi.ornl.gov). Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the US Department of Energy under contract DEAC05-00OR22725.

Funders	Funder number
US Department of Energy
US State Department
National Science Foundation	1248096, 0960114
American Association of Immunologists
Office of Science
Biological and Environmental Research
Oak Ridge National Laboratory	DEAC05-00OR22725

Keywords

Chryseobacterium
Endosphere
Populus deltoides

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10.1099/ijsem.0.003140

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title = "Chryseobacterium populi sp. nov., isolated from Populus deltoides endosphere",

abstract = " As part of a study investigating the rhizosphere and endosphere of the Eastern cottonwood tree, Populus deltoides, a number of isolates were subjected to genome sequencing. The genome-derived 16S rRNA gene sequence of strain CF314 T was 97.0% similar to those of the Chryseobacterium daecheongense and Chryseobacterium polytrichastri type strains, but was essentially equidistant from many other Chryseobacterium type strains. Overall genome similarity metrics (average nucleotide identity, digital DNA-DNA hybridization, average amino acid identity) revealed greatest similarity to the Chryseobacterium daecheongense, Chryseobacterium piperi and Chryseobacterium soldanellicola type strains, but were well below the species thresholds. Strain CF314 T had a typical fatty acid composition for Chryseobacterium species and produced flexirubin pigments, but not carotenoids. The genome encodes a number of proteins such as a C-type lectin and terpene synthases that are also found in other plant-associated Bacteroidetes. Based on phenotypic and genomic characteristics of the strain, we propose the new species Chryseobacterium populi. The type strain is CF314 T =KCTC 62722 T =LMG 30786 T .",

keywords = "Chryseobacterium, Endosphere, Populus deltoides",

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Chryseobacterium populi sp. nov., isolated from Populus deltoides endosphere

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