Chromosome aberrations induced in human lymphocytes by U-235 fission neutrons: I. Irradiation of human blood samples in the 'dry cell' of the TRIGA Mark II nuclear reactor

A. Fajgelj; A. Lakoski; D. Horvat; I. Remec; J. Skrk; P. Stegnar

Chromosome aberrations induced in human lymphocytes by U-235 fission neutrons: I. Irradiation of human blood samples in the 'dry cell' of the TRIGA Mark II nuclear reactor

A. Fajgelj, A. Lakoski, D. Horvat, I. Remec, J. Skrk, P. Stegnar

Research output: Contribution to journal › Article › peer-review

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Abstract

A set-up for irradiation of biological samples in the TRIGA Mark II research reactor in Ljubljana is described. Threshold activation detectors were used for characterisation of the neutron flux, and the accompanying gamma dose was measured by TLDs. Human peripheral blood samples were irradiated 'in vitro' and biological effects evaluated according to the unstable chromosomal aberrations induced. Biological effects of two types of cultivation of irradiated blood samples, the first immediately after irradiation and the second after 96 h storage, were studied. A significant difference in the incidence of chromosomal aberrations between these two types of samples was obtained, while our dose-response curve fitting coefficients α₁ = (7.71 ± 0.09) x 10^-2 Gy^-1 (immediate cultivation) and α₂ = (11.03 ± 0.08) x 10^-2 Gy^-1 (96 h delayed cultivation) are in both cases lower than could be found in the literature.

Original language	English
Pages (from-to)	661-666
Number of pages	6
Journal	Strahlentherapie und Onkologie
Volume	167
Issue number	11
State	Published - 1991
Externally published	Yes

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title = "Chromosome aberrations induced in human lymphocytes by U-235 fission neutrons: I. Irradiation of human blood samples in the 'dry cell' of the TRIGA Mark II nuclear reactor",

abstract = "A set-up for irradiation of biological samples in the TRIGA Mark II research reactor in Ljubljana is described. Threshold activation detectors were used for characterisation of the neutron flux, and the accompanying gamma dose was measured by TLDs. Human peripheral blood samples were irradiated 'in vitro' and biological effects evaluated according to the unstable chromosomal aberrations induced. Biological effects of two types of cultivation of irradiated blood samples, the first immediately after irradiation and the second after 96 h storage, were studied. A significant difference in the incidence of chromosomal aberrations between these two types of samples was obtained, while our dose-response curve fitting coefficients α1 = (7.71 ± 0.09) x 10-2 Gy-1 (immediate cultivation) and α2 = (11.03 ± 0.08) x 10-2 Gy-1 (96 h delayed cultivation) are in both cases lower than could be found in the literature.",

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T2 - I. Irradiation of human blood samples in the 'dry cell' of the TRIGA Mark II nuclear reactor

AU - Fajgelj, A.

AU - Lakoski, A.

AU - Horvat, D.

AU - Remec, I.

AU - Skrk, J.

AU - Stegnar, P.

PY - 1991

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AB - A set-up for irradiation of biological samples in the TRIGA Mark II research reactor in Ljubljana is described. Threshold activation detectors were used for characterisation of the neutron flux, and the accompanying gamma dose was measured by TLDs. Human peripheral blood samples were irradiated 'in vitro' and biological effects evaluated according to the unstable chromosomal aberrations induced. Biological effects of two types of cultivation of irradiated blood samples, the first immediately after irradiation and the second after 96 h storage, were studied. A significant difference in the incidence of chromosomal aberrations between these two types of samples was obtained, while our dose-response curve fitting coefficients α1 = (7.71 ± 0.09) x 10-2 Gy-1 (immediate cultivation) and α2 = (11.03 ± 0.08) x 10-2 Gy-1 (96 h delayed cultivation) are in both cases lower than could be found in the literature.

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Chromosome aberrations induced in human lymphocytes by U-235 fission neutrons: I. Irradiation of human blood samples in the 'dry cell' of the TRIGA Mark II nuclear reactor

Abstract

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