Chromatographic separation of germanium and arsenic for the production of high purity 77As

Matthew D. Gott; Anthony J. DeGraffenreid; Yutian Feng; Michael D. Phipps; Donald E. Wycoff; Mary F. Embree; Cathy S. Cutler; Alan R. Ketring; Silvia S. Jurisson

doi:10.1016/j.chroma.2016.02.074

Chromatographic separation of germanium and arsenic for the production of high purity ⁷⁷As

Matthew D. Gott
, Anthony J. DeGraffenreid
, Yutian Feng
, Michael D. Phipps
, Donald E. Wycoff
, Mary F. Embree
, Cathy S. Cutler
, Alan R. Ketring
, Silvia S. Jurisson

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A simple column chromatographic method was developed to isolate ⁷⁷As (94 ± 6% (EtOH/HCl); 74 ± 11 (MeOH)) from germanium for potential use in radioimmunotherapy. The separation of arsenic from germanium was based on their relative affinities for different chromatographic materials in aqueous and organic environments. Using an organic or mixed mobile phase, germanium was selectively retained on a silica gel column as germanate, while arsenic was eluted from the column as arsenate. Subsequently, enriched ⁷⁶Ge (98 ± 2) was recovered for reuse by elution with aqueous solution (neutral to basic). Greater than 98% radiolabeling yield of a ⁷⁷As-trithiol was observed from methanol separated [⁷⁷As]arsenate [17].

Original language	English
Pages (from-to)	68-74
Number of pages	7
Journal	Journal of Chromatography A
Volume	1441
DOIs	https://doi.org/10.1016/j.chroma.2016.02.074
State	Published - Apr 8 2016
Externally published	Yes

Funding

The authors acknowledge support from the Department of Energy, Office of Basic Sciences, Isotope Research Program under grants DE-SC0003851 and DE-SC0010283 . Additional trainee support was provided from the National Science Foundation under IGERT award DGE-0965983 (M.D. Gott) and the National Institutes of Health under NIBIB Training Grant 5 T32-EB004822 (A.J. DeGraffenreid).

Keywords

Column chromatography
Ge/As distribution coefficient
Ge/As separation
High specific activity arsenic-77

Access to Document

10.1016/j.chroma.2016.02.074

Cite this

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title = "Chromatographic separation of germanium and arsenic for the production of high purity 77As",

abstract = "A simple column chromatographic method was developed to isolate 77As (94 ± 6\% (EtOH/HCl); 74 ± 11 (MeOH)) from germanium for potential use in radioimmunotherapy. The separation of arsenic from germanium was based on their relative affinities for different chromatographic materials in aqueous and organic environments. Using an organic or mixed mobile phase, germanium was selectively retained on a silica gel column as germanate, while arsenic was eluted from the column as arsenate. Subsequently, enriched 76Ge (98 ± 2) was recovered for reuse by elution with aqueous solution (neutral to basic). Greater than 98\% radiolabeling yield of a 77As-trithiol was observed from methanol separated [77As]arsenate [17].",

keywords = "Column chromatography, Ge/As distribution coefficient, Ge/As separation, High specific activity arsenic-77",

author = "Gott, \{Matthew D.\} and DeGraffenreid, \{Anthony J.\} and Yutian Feng and Phipps, \{Michael D.\} and Wycoff, \{Donald E.\} and Embree, \{Mary F.\} and Cutler, \{Cathy S.\} and Ketring, \{Alan R.\} and Jurisson, \{Silvia S.\}",

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doi = "10.1016/j.chroma.2016.02.074",

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AU - Gott, Matthew D.

AU - DeGraffenreid, Anthony J.

AU - Feng, Yutian

AU - Phipps, Michael D.

AU - Wycoff, Donald E.

AU - Embree, Mary F.

AU - Cutler, Cathy S.

AU - Ketring, Alan R.

AU - Jurisson, Silvia S.

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AB - A simple column chromatographic method was developed to isolate 77As (94 ± 6% (EtOH/HCl); 74 ± 11 (MeOH)) from germanium for potential use in radioimmunotherapy. The separation of arsenic from germanium was based on their relative affinities for different chromatographic materials in aqueous and organic environments. Using an organic or mixed mobile phase, germanium was selectively retained on a silica gel column as germanate, while arsenic was eluted from the column as arsenate. Subsequently, enriched 76Ge (98 ± 2) was recovered for reuse by elution with aqueous solution (neutral to basic). Greater than 98% radiolabeling yield of a 77As-trithiol was observed from methanol separated [77As]arsenate [17].

KW - Column chromatography

KW - Ge/As distribution coefficient

KW - Ge/As separation

KW - High specific activity arsenic-77

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