Cholesterol's location in lipid bilayers

Drew Marquardt; Norbert Kučerka; Stephen R. Wassall; Thad A. Harroun; John Katsaras

doi:10.1016/j.chemphyslip.2016.04.001

Cholesterol's location in lipid bilayers

Drew Marquardt, Norbert Kučerka, Stephen R. Wassall, Thad A. Harroun, John Katsaras

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79 Scopus citations

Abstract

It is well known that cholesterol modifies the physical properties of lipid bilayers. For example, the much studied liquid-ordered L_o phase contains rapidly diffusing lipids with their acyl chains in the all trans configuration, similar to gel phase bilayers. Moreover, the L_o phase is commonly associated with cholesterol-enriched lipid rafts, which are thought to serve as platforms for signaling proteins in the plasma membrane. Cholesterol's location in lipid bilayers has been studied extensively, and it has been shown – at least in some bilayers – to align differently from its canonical upright orientation, where its hydroxyl group is in the vicinity of the lipid–water interface. In this article we review recent works describing cholesterol's location in different model membrane systems with emphasis on results obtained from scattering, spectroscopic and molecular dynamics studies.

Original language	English
Pages (from-to)	17-25
Number of pages	9
Journal	Chemistry and Physics of Lipids
Volume	199
DOIs	https://doi.org/10.1016/j.chemphyslip.2016.04.001
State	Published - Sep 1 2016

Funding

DM thanks Georg Pabst for his support. NK is supported through the VEGA grant 1/0916/16 and collaborative SR-JINR program under theme 04-4-1121-2015/2017 , JK is supported through the Scientific User Facilities Division of the DOE Office of Basic Energy Sciences under US DOE Contract No. DE-AC05-00OR22725 .

Funders	Funder number
DOE Office of Basic Energy Sciences
SR-JINR	04-4-1121-2015/2017
U.S. Department of Energy	DE-AC05-00OR22725
U.S. Department of Energy
Vedecká Grantová Agentúra MŠVVaŠ SR a SAV	1/0916/16
Vedecká Grantová Agentúra MŠVVaŠ SR a SAV

Keywords

Cholesterol
Lipid domains
Membrane dynamics
Membrane structure
Sterol

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10.1016/j.chemphyslip.2016.04.001

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title = "Cholesterol's location in lipid bilayers",

abstract = "It is well known that cholesterol modifies the physical properties of lipid bilayers. For example, the much studied liquid-ordered Lo phase contains rapidly diffusing lipids with their acyl chains in the all trans configuration, similar to gel phase bilayers. Moreover, the Lo phase is commonly associated with cholesterol-enriched lipid rafts, which are thought to serve as platforms for signaling proteins in the plasma membrane. Cholesterol's location in lipid bilayers has been studied extensively, and it has been shown – at least in some bilayers – to align differently from its canonical upright orientation, where its hydroxyl group is in the vicinity of the lipid–water interface. In this article we review recent works describing cholesterol's location in different model membrane systems with emphasis on results obtained from scattering, spectroscopic and molecular dynamics studies.",

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Cholesterol's location in lipid bilayers

Abstract

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Keywords

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