TY - JOUR
T1 - Characterization of interactions between and among components of the meiotic silencing by unpaired DNA machinery in Neurospora crassa using bimolecular fluorescence complementation
AU - Bardiya, Nirmala
AU - Alexander, William G.
AU - Perdue, Tony D.
AU - Barry, Edward G.
AU - Metzenberg, Robert L.
AU - Pukkila, Patricia J.
AU - Shiu, Patrick K.T.
PY - 2008/1
Y1 - 2008/1
N2 - Bimolecular fluorescence complementation (BiFC) is based on the complementation between two nonfluorescent fragments of the yellow fluorescent protein (YFP) when they are united by interactions between proteins covalently linked to them. We have successfully applied BiFC in Neurospora crassa using two genes involved in meiotic silencing by unpaired DNA (MSUD) and observed macromolecular complex formation involving only SAD-1 proteins, only SAD-2 proteins, and mixtures of SAD-1 and SAD-2 proteins.
AB - Bimolecular fluorescence complementation (BiFC) is based on the complementation between two nonfluorescent fragments of the yellow fluorescent protein (YFP) when they are united by interactions between proteins covalently linked to them. We have successfully applied BiFC in Neurospora crassa using two genes involved in meiotic silencing by unpaired DNA (MSUD) and observed macromolecular complex formation involving only SAD-1 proteins, only SAD-2 proteins, and mixtures of SAD-1 and SAD-2 proteins.
UR - http://www.scopus.com/inward/record.url?scp=40849086680&partnerID=8YFLogxK
U2 - 10.1534/genetics.107.079384
DO - 10.1534/genetics.107.079384
M3 - Article
C2 - 18202398
AN - SCOPUS:40849086680
SN - 0016-6731
VL - 178
SP - 593
EP - 596
JO - Genetics
JF - Genetics
IS - 1
ER -