Characterization of DWARF14 Genes in Populus

Kaijie Zheng; Xiaoping Wang; Deborah A. Weighill; Hao Bo Guo; Meng Xie; Yongil Yang; Jun Yang; Shucai Wang; Daniel A. Jacobson; Hong Guo; Wellington Muchero; Gerald A. Tuskan; Jin Gui Chen

doi:10.1038/srep21593

Characterization of DWARF14 Genes in Populus

Kaijie Zheng, Xiaoping Wang, Deborah A. Weighill, Hao Bo Guo, Meng Xie, Yongil Yang, Jun Yang, Shucai Wang, Daniel A. Jacobson, Hong Guo, Wellington Muchero, Gerald A. Tuskan, Jin Gui Chen

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Strigolactones are a new class of plant hormones regulating shoot branching and symbiotic interactions with arbuscular mycorrhizal fungi. Studies of branching mutants in herbaceous plants have identified several key genes involved in strigolactone biosynthesis or signaling. The strigolactone signal is perceived by a member of the α/β-fold hydrolase superfamily, known as DWARF14 (D14). However, little is known about D14 genes in the woody perennial plants. Here we report the identification of D14 homologs in the model woody plant Populus trichocarpa. We showed that there are two D14 homologs in P. trichocarpa, designated as PtD14a and PtD14b that are over 95% similar at the amino acid level. Expression analysis indicated that the transcript level of PtD14a is generally more abundant than that of PtD14b. However, only PtD14a was able to complement Arabidopsis d14 mutants, suggesting that PtD14a is the functional D14 ortholog. Amino acid alignment and structural modeling revealed substitutions of several highly conserved amino acids in the PtD14b protein including a phenylalanine near the catalytic triad of D14 proteins. This study lays a foundation for further characterization of strigolactone pathway and its functions in the woody perennial plants.

Original language	English
Article number	21593
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep21593
State	Published - Feb 15 2016

Funding

This work was supported by the Plant-Microbe Interfaces Scientific Focus Area in the Genomic Science Program, the Office of Biological and Environmental Research in the U.S. Department of Energy Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the United States Department of Energy under contract DE-AC05-00OR22725. K.Z. and X.W. were partially supported by visiting scholarships from the China Scholarship Council. J.Y. was partially supported by a visiting scholarship from the Chinese Academy of Sciences (Grant Number: 201019).

Funders	Funder number
Plant-Microbe Interfaces Scientific Focus Area
U.S. Department of Energy
Biological and Environmental Research
Chinese Academy of Sciences	201019
China Scholarship Council

Access to Document

10.1038/srep21593

Cite this

@article{e30acb7fcaed4797ac4df2b19f50d7e8,

title = "Characterization of DWARF14 Genes in Populus",

abstract = "Strigolactones are a new class of plant hormones regulating shoot branching and symbiotic interactions with arbuscular mycorrhizal fungi. Studies of branching mutants in herbaceous plants have identified several key genes involved in strigolactone biosynthesis or signaling. The strigolactone signal is perceived by a member of the α/β-fold hydrolase superfamily, known as DWARF14 (D14). However, little is known about D14 genes in the woody perennial plants. Here we report the identification of D14 homologs in the model woody plant Populus trichocarpa. We showed that there are two D14 homologs in P. trichocarpa, designated as PtD14a and PtD14b that are over 95% similar at the amino acid level. Expression analysis indicated that the transcript level of PtD14a is generally more abundant than that of PtD14b. However, only PtD14a was able to complement Arabidopsis d14 mutants, suggesting that PtD14a is the functional D14 ortholog. Amino acid alignment and structural modeling revealed substitutions of several highly conserved amino acids in the PtD14b protein including a phenylalanine near the catalytic triad of D14 proteins. This study lays a foundation for further characterization of strigolactone pathway and its functions in the woody perennial plants.",

author = "Kaijie Zheng and Xiaoping Wang and Weighill, {Deborah A.} and Guo, {Hao Bo} and Meng Xie and Yongil Yang and Jun Yang and Shucai Wang and Jacobson, {Daniel A.} and Hong Guo and Wellington Muchero and Tuskan, {Gerald A.} and Chen, {Jin Gui}",

year = "2016",

month = feb,

day = "15",

doi = "10.1038/srep21593",

language = "English",

volume = "6",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

}

TY - JOUR

T1 - Characterization of DWARF14 Genes in Populus

AU - Zheng, Kaijie

AU - Wang, Xiaoping

AU - Weighill, Deborah A.

AU - Guo, Hao Bo

AU - Xie, Meng

AU - Yang, Yongil

AU - Yang, Jun

AU - Wang, Shucai

AU - Jacobson, Daniel A.

AU - Guo, Hong

AU - Muchero, Wellington

AU - Tuskan, Gerald A.

AU - Chen, Jin Gui

PY - 2016/2/15

Y1 - 2016/2/15

N2 - Strigolactones are a new class of plant hormones regulating shoot branching and symbiotic interactions with arbuscular mycorrhizal fungi. Studies of branching mutants in herbaceous plants have identified several key genes involved in strigolactone biosynthesis or signaling. The strigolactone signal is perceived by a member of the α/β-fold hydrolase superfamily, known as DWARF14 (D14). However, little is known about D14 genes in the woody perennial plants. Here we report the identification of D14 homologs in the model woody plant Populus trichocarpa. We showed that there are two D14 homologs in P. trichocarpa, designated as PtD14a and PtD14b that are over 95% similar at the amino acid level. Expression analysis indicated that the transcript level of PtD14a is generally more abundant than that of PtD14b. However, only PtD14a was able to complement Arabidopsis d14 mutants, suggesting that PtD14a is the functional D14 ortholog. Amino acid alignment and structural modeling revealed substitutions of several highly conserved amino acids in the PtD14b protein including a phenylalanine near the catalytic triad of D14 proteins. This study lays a foundation for further characterization of strigolactone pathway and its functions in the woody perennial plants.

AB - Strigolactones are a new class of plant hormones regulating shoot branching and symbiotic interactions with arbuscular mycorrhizal fungi. Studies of branching mutants in herbaceous plants have identified several key genes involved in strigolactone biosynthesis or signaling. The strigolactone signal is perceived by a member of the α/β-fold hydrolase superfamily, known as DWARF14 (D14). However, little is known about D14 genes in the woody perennial plants. Here we report the identification of D14 homologs in the model woody plant Populus trichocarpa. We showed that there are two D14 homologs in P. trichocarpa, designated as PtD14a and PtD14b that are over 95% similar at the amino acid level. Expression analysis indicated that the transcript level of PtD14a is generally more abundant than that of PtD14b. However, only PtD14a was able to complement Arabidopsis d14 mutants, suggesting that PtD14a is the functional D14 ortholog. Amino acid alignment and structural modeling revealed substitutions of several highly conserved amino acids in the PtD14b protein including a phenylalanine near the catalytic triad of D14 proteins. This study lays a foundation for further characterization of strigolactone pathway and its functions in the woody perennial plants.

UR - http://www.scopus.com/inward/record.url?scp=84958212546&partnerID=8YFLogxK

U2 - 10.1038/srep21593

DO - 10.1038/srep21593

M3 - Article

C2 - 26875827

AN - SCOPUS:84958212546

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 21593

ER -

Characterization of DWARF14 Genes in Populus

Abstract

Funding

Access to Document

Other files and links

Fingerprint

Cite this