Characteristics of lignin fractions from dilute acid pretreated switchgrass and their effect on cellobiohydrolase from Trichoderma longibrachiatum

Lan Yao; Haitao Yang; Chang Geun Yoo; Xianzhi Meng; Yunqiao Pu; Naijia Hao; Arthur J. Ragauskas

doi:10.3389/fenrg.2018.00001

Characteristics of lignin fractions from dilute acid pretreated switchgrass and their effect on cellobiohydrolase from Trichoderma longibrachiatum

Lan Yao, Haitao Yang, Chang Geun Yoo, Xianzhi Meng, Yunqiao Pu, Naijia Hao, Arthur J. Ragauskas

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

To investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H₂O extraction, and (iii) cellulase treatment, respectively. Structural properties of each lignin fraction were elucidated by GPC, ¹³C-NMR, and 2D-HSQC NMR analyses. The adsorptions of CBH to the isolated lignin fractions were also studied by Langmuir adsorption isotherms. Ethanol-extractable lignin fraction, mainly composed of syringyl (S) and guaiacyl (G) units, had the lowest molecular weight, while dioxane/H₂O-extracted lignin fraction had the lowest S/G ratio with higher content of p-coumaric acid (pCA) unit. The residual lignin fraction after enzymatic treatment had the highest S/G ratio without hydroxyphenyl (H) unit. Strong associations were found between lignin properties such as lignin composition and S/G ratio and its non-productive enzyme adsorption factors including the maximum adsorption capacity and binding strength.

Original language	English
Article number	1
Journal	Frontiers in Energy Research
Volume	6
Issue number	FEB
DOIs	https://doi.org/10.3389/fenrg.2018.00001
State	Published - Feb 6 2018
Externally published	Yes

Funding

LY and HY are grateful for the support by National Natural Science Foundation of China (No. 31500496), China Scholarship Council (NO. 2011842330 and NO. 201508420257) and Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education of China (NO. KF-201719 and NO. KF201611), State Key Laboratory of Pulp and Paper Engineering (No. 201510), key project of Hubei Provincial Department of Education (NO.D20161402) and Foundation (No. 201611B01) of Hubei Provincial Key Laboratory of Green Materials for Light Industry. This manuscript has been authored and partially supported by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the U.S. Department of Energy. This study was supported and performed as part of the BioEnergy Science Center (BESC). The BESC is a U.S Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan). The views and opinions of the authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. Neither the United States Government nor any agency thereof, nor any of their employes, makes any warranty, expressed or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights.

Funders	Funder number
BioEnergy Science Center
DOE Public Access Plan
Department of Energy Bioenergy Research Center
Hubei Provincial Key Laboratory of Green Materials for Light Industry
Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education of China	KF201611, KF-201719
United States Government
U.S. Department of Energy
Office of Science
Biological and Environmental Research
Hubei Provincial Department of Education	201611B01, NO.D20161402
UT-Battelle	DE-AC05-00OR22725
National Natural Science Foundation of China	31500496
China Scholarship Council	2011842330, 201508420257
State Key Laboratory of Pulp and Paper Engineering	201510

Keywords

Cellobiohydrolase
Characterization
Dilute acid pretreatment
Lignin
Switchgrass

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10.3389/fenrg.2018.00001

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title = "Characteristics of lignin fractions from dilute acid pretreated switchgrass and their effect on cellobiohydrolase from Trichoderma longibrachiatum",

abstract = "To investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O extraction, and (iii) cellulase treatment, respectively. Structural properties of each lignin fraction were elucidated by GPC, 13C-NMR, and 2D-HSQC NMR analyses. The adsorptions of CBH to the isolated lignin fractions were also studied by Langmuir adsorption isotherms. Ethanol-extractable lignin fraction, mainly composed of syringyl (S) and guaiacyl (G) units, had the lowest molecular weight, while dioxane/H2O-extracted lignin fraction had the lowest S/G ratio with higher content of p-coumaric acid (pCA) unit. The residual lignin fraction after enzymatic treatment had the highest S/G ratio without hydroxyphenyl (H) unit. Strong associations were found between lignin properties such as lignin composition and S/G ratio and its non-productive enzyme adsorption factors including the maximum adsorption capacity and binding strength.",

keywords = "Cellobiohydrolase, Characterization, Dilute acid pretreatment, Lignin, Switchgrass",

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AU - Yoo, Chang Geun

AU - Meng, Xianzhi

AU - Pu, Yunqiao

AU - Hao, Naijia

AU - Ragauskas, Arthur J.

PY - 2018/2/6

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N2 - To investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O extraction, and (iii) cellulase treatment, respectively. Structural properties of each lignin fraction were elucidated by GPC, 13C-NMR, and 2D-HSQC NMR analyses. The adsorptions of CBH to the isolated lignin fractions were also studied by Langmuir adsorption isotherms. Ethanol-extractable lignin fraction, mainly composed of syringyl (S) and guaiacyl (G) units, had the lowest molecular weight, while dioxane/H2O-extracted lignin fraction had the lowest S/G ratio with higher content of p-coumaric acid (pCA) unit. The residual lignin fraction after enzymatic treatment had the highest S/G ratio without hydroxyphenyl (H) unit. Strong associations were found between lignin properties such as lignin composition and S/G ratio and its non-productive enzyme adsorption factors including the maximum adsorption capacity and binding strength.

AB - To investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O extraction, and (iii) cellulase treatment, respectively. Structural properties of each lignin fraction were elucidated by GPC, 13C-NMR, and 2D-HSQC NMR analyses. The adsorptions of CBH to the isolated lignin fractions were also studied by Langmuir adsorption isotherms. Ethanol-extractable lignin fraction, mainly composed of syringyl (S) and guaiacyl (G) units, had the lowest molecular weight, while dioxane/H2O-extracted lignin fraction had the lowest S/G ratio with higher content of p-coumaric acid (pCA) unit. The residual lignin fraction after enzymatic treatment had the highest S/G ratio without hydroxyphenyl (H) unit. Strong associations were found between lignin properties such as lignin composition and S/G ratio and its non-productive enzyme adsorption factors including the maximum adsorption capacity and binding strength.

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Characteristics of lignin fractions from dilute acid pretreated switchgrass and their effect on cellobiohydrolase from Trichoderma longibrachiatum

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