TY - JOUR
T1 - Characteristics of lignin fractions from dilute acid pretreated switchgrass and their effect on cellobiohydrolase from Trichoderma longibrachiatum
AU - Yao, Lan
AU - Yang, Haitao
AU - Yoo, Chang Geun
AU - Meng, Xianzhi
AU - Pu, Yunqiao
AU - Hao, Naijia
AU - Ragauskas, Arthur J.
N1 - Publisher Copyright:
© 2018 Yao, Yang, Yoo, Meng, Pu, Hao and Ragauskas.
PY - 2018/2/6
Y1 - 2018/2/6
N2 - To investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O extraction, and (iii) cellulase treatment, respectively. Structural properties of each lignin fraction were elucidated by GPC, 13C-NMR, and 2D-HSQC NMR analyses. The adsorptions of CBH to the isolated lignin fractions were also studied by Langmuir adsorption isotherms. Ethanol-extractable lignin fraction, mainly composed of syringyl (S) and guaiacyl (G) units, had the lowest molecular weight, while dioxane/H2O-extracted lignin fraction had the lowest S/G ratio with higher content of p-coumaric acid (pCA) unit. The residual lignin fraction after enzymatic treatment had the highest S/G ratio without hydroxyphenyl (H) unit. Strong associations were found between lignin properties such as lignin composition and S/G ratio and its non-productive enzyme adsorption factors including the maximum adsorption capacity and binding strength.
AB - To investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O extraction, and (iii) cellulase treatment, respectively. Structural properties of each lignin fraction were elucidated by GPC, 13C-NMR, and 2D-HSQC NMR analyses. The adsorptions of CBH to the isolated lignin fractions were also studied by Langmuir adsorption isotherms. Ethanol-extractable lignin fraction, mainly composed of syringyl (S) and guaiacyl (G) units, had the lowest molecular weight, while dioxane/H2O-extracted lignin fraction had the lowest S/G ratio with higher content of p-coumaric acid (pCA) unit. The residual lignin fraction after enzymatic treatment had the highest S/G ratio without hydroxyphenyl (H) unit. Strong associations were found between lignin properties such as lignin composition and S/G ratio and its non-productive enzyme adsorption factors including the maximum adsorption capacity and binding strength.
KW - Cellobiohydrolase
KW - Characterization
KW - Dilute acid pretreatment
KW - Lignin
KW - Switchgrass
UR - http://www.scopus.com/inward/record.url?scp=85042755617&partnerID=8YFLogxK
U2 - 10.3389/fenrg.2018.00001
DO - 10.3389/fenrg.2018.00001
M3 - Article
AN - SCOPUS:85042755617
SN - 2296-598X
VL - 6
JO - Frontiers in Energy Research
JF - Frontiers in Energy Research
IS - FEB
M1 - 1
ER -