Changes in vibrational modes of water and bioprotectants in solution

S. Magazù; F. Migliardo; A. J. Ramirez-Cuesta

doi:10.1016/j.bpc.2006.07.003

Changes in vibrational modes of water and bioprotectants in solution

S. Magazù, F. Migliardo, A. J. Ramirez-Cuesta

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Inelastic neutron scattering (INS) measurements have been performed on trehalose and sucrose/H₂O mixtures at very low temperature as a function of concentration by using the TOSCA spectrometer at the ISIS Facility (DRAL, UK). The aim of this work is to investigate by INS the vibrational behaviour of water in presence of trehalose and sucrose in order to characterize the changes induced by these disaccharides on the H₂O hydrogen-bonded network. In particular, we obtained information about the effects of the two disaccharides in the translational, librational and bending spectral regions of ice. The disaccharide bioprotective effectiveness can be linked by the high destructuring effect emphasised by the analysis of the librational modes region. On the other hand, the analysis of the vibrational region corresponding to the ice bending modes show a high "crystallinity" degree which can justify the cryptobiotic action of disaccharides.

Original language	English
Pages (from-to)	138-142
Number of pages	5
Journal	Biophysical Chemistry
Volume	125
Issue number	1
DOIs	https://doi.org/10.1016/j.bpc.2006.07.003
State	Published - Jan 2007
Externally published	Yes

Keywords

Bioprotection
Disaccharides
Neutron scattering
Vibrational properties
Water mixtures

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10.1016/j.bpc.2006.07.003

Cite this

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title = "Changes in vibrational modes of water and bioprotectants in solution",

abstract = "Inelastic neutron scattering (INS) measurements have been performed on trehalose and sucrose/H2O mixtures at very low temperature as a function of concentration by using the TOSCA spectrometer at the ISIS Facility (DRAL, UK). The aim of this work is to investigate by INS the vibrational behaviour of water in presence of trehalose and sucrose in order to characterize the changes induced by these disaccharides on the H2O hydrogen-bonded network. In particular, we obtained information about the effects of the two disaccharides in the translational, librational and bending spectral regions of ice. The disaccharide bioprotective effectiveness can be linked by the high destructuring effect emphasised by the analysis of the librational modes region. On the other hand, the analysis of the vibrational region corresponding to the ice bending modes show a high {"}crystallinity{"} degree which can justify the cryptobiotic action of disaccharides.",

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author = "S. Magaz{\`u} and F. Migliardo and Ramirez-Cuesta, {A. J.}",

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T1 - Changes in vibrational modes of water and bioprotectants in solution

AU - Magazù, S.

AU - Migliardo, F.

AU - Ramirez-Cuesta, A. J.

PY - 2007/1

Y1 - 2007/1

N2 - Inelastic neutron scattering (INS) measurements have been performed on trehalose and sucrose/H2O mixtures at very low temperature as a function of concentration by using the TOSCA spectrometer at the ISIS Facility (DRAL, UK). The aim of this work is to investigate by INS the vibrational behaviour of water in presence of trehalose and sucrose in order to characterize the changes induced by these disaccharides on the H2O hydrogen-bonded network. In particular, we obtained information about the effects of the two disaccharides in the translational, librational and bending spectral regions of ice. The disaccharide bioprotective effectiveness can be linked by the high destructuring effect emphasised by the analysis of the librational modes region. On the other hand, the analysis of the vibrational region corresponding to the ice bending modes show a high "crystallinity" degree which can justify the cryptobiotic action of disaccharides.

AB - Inelastic neutron scattering (INS) measurements have been performed on trehalose and sucrose/H2O mixtures at very low temperature as a function of concentration by using the TOSCA spectrometer at the ISIS Facility (DRAL, UK). The aim of this work is to investigate by INS the vibrational behaviour of water in presence of trehalose and sucrose in order to characterize the changes induced by these disaccharides on the H2O hydrogen-bonded network. In particular, we obtained information about the effects of the two disaccharides in the translational, librational and bending spectral regions of ice. The disaccharide bioprotective effectiveness can be linked by the high destructuring effect emphasised by the analysis of the librational modes region. On the other hand, the analysis of the vibrational region corresponding to the ice bending modes show a high "crystallinity" degree which can justify the cryptobiotic action of disaccharides.

KW - Bioprotection

KW - Disaccharides

KW - Neutron scattering

KW - Vibrational properties

KW - Water mixtures

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DO - 10.1016/j.bpc.2006.07.003

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SN - 0301-4622

VL - 125

SP - 138

EP - 142

JO - Biophysical Chemistry

JF - Biophysical Chemistry

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Changes in vibrational modes of water and bioprotectants in solution

Abstract

Keywords

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