Changes Experienced by Low-Concentration Lipid Bicelles as a Function of Temperature

Ibtihal Alahmadi, Donyeil Hoy, Armin Tahmasbi Rad, Sanyukta Patil, Anas Alahmadi, Jacob Kinnun, Haden L. Scott, John Katsaras, Mu Ping Nieh

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Differential scanning calorimetry (DSC) of dipalmitoyl phosphatidylcholine (DPPC), dihexanoyl phosphatidylcholine, and dipalmitoyl phosphatidylglycerol bicelles reveals two endothermic peaks. Based on analysis of small angle neutron scattering and small angle X-ray scattering data, the two DSC peaks are associated with the melting of DPPC and a change in bicellar morphology─namely, either bicelle-to-spherical vesicle or oblate-to-spherical vesicle. The reversibility of the two structural transformations was examined by DSC and found to be consistent with the corresponding small angle scattering data. However, the peak that is not associated with the melting of DPPC does not correspond to any structural transformation for bicelles containing distearoyl phosphatidylethanolamine conjugated with polyethylene glycol. Based on complementary experimental data, we conclude that membrane flexibility, lipid miscibility, and differential solubility between the long- and short-chain lipids in water are important parameters controlling the reversibility of morphologies experienced by the bicelles.

Original languageEnglish
Pages (from-to)4332-4340
Number of pages9
JournalLangmuir
Volume38
Issue number14
DOIs
StatePublished - Apr 12 2022

Funding

High-sensitivity DSC was acquired through the support of the NSF-MRI program (NSF-MRI 1228817). I.A. was supported by the Ministry of Education in Saudi Arabia. D.H., Jr. was supported by the US Department of Education GAANN (Award Number P200A150330), National Science Foundation Graduate Research Fellowship under Grant No. DGE-1747453, Giolas-Harriot/Crandall-Cordero and GE fellowships at the University of Connecticut. I.A. would also like to acknowledge the GE fellowship support at the University of Connecticut. A.T.R. was partially supported by NSF-CBET 1605971, which also covers the cost of the materials. We would also like to thank Dr. Kuo-Chih Shih and Mr. Behrad Kangarlou for their discussion and assistance on this project. The SAXS data were collected with the help of Dr. Lin Yang, at the 16ID-LiX Beamline, National Synchrotron Light Source II, Brookhaven National Laboratory (BNL), NY, USA, through a beamtime proposal (BAG-302208). The LiX beamline is part of the Life Science Biomedical Technology Research resource, jointly supported by the National Institutes of Health, National Institute of General Medical Sciences, under Grant P41 GM111244, and by the Department of Energy Office of Biological and Environmental Research under Grant KP1605010, with additional support from NIH Grant S10 OD012331. NSLS-II is a U.S. Department of Energy (DOE) Office of Science User Facility, operated for the DOE Office of Science by Brookhaven National Laboratory under Contract No. DESC0012704. J.K., J.K., and H.S. were supported through the Scientific User Facilities Division of the DOE Office of Basic Energy Sciences under US DOE Contract No. DE-AC05-00OR22725. High-sensitivity DSC was acquired through the support of the NSF-MRI program (NSF-MRI 1228817). I.A. was supported by the Ministry of Education in Saudi Arabia. D.H., Jr. was supported by the US Department of Education GAANN (Award Number P200A150330), National Science Foundation Graduate Research Fellowship under Grant No. DGE-1747453 Giolas-Harriot/Crandall-Cordero and GE fellowships at the University of Connecticut. I.A. would also like to acknowledge the GE fellowship support at the University of Connecticut. A.T.R. was partially supported by NSF-CBET 1605971, which also covers the cost of the materials. We would also like to thank Dr. Kuo-Chih Shih and Mr. Behrad Kangarlou for their discussion and assistance on this project. The SAXS data were collected with the help of Dr. Lin Yang, at the 16ID-LiX Beamline National Synchrotron Light Source II, Brookhaven National Laboratory (BNL), NY, USA, through a beamtime proposal (BAG-302208). The LiX beamline is part of the Life Science Biomedical Technology Research resource, jointly supported by the National Institutes of Health National Institute of General Medical Sciences, under Grant P41 GM111244 and by the Department of Energy Office of Biological and Environmental Research under Grant KP1605010, with additional support from NIH Grant S10 OD012331. NSLS-II is a U.S. Department of Energy (DOE) Office of Science User Facility, operated for the DOE Office of Science by Brookhaven National Laboratory under Contract No. DESC0012704. J.K. J.K., and H.S. were supported through the Scientific User Facilities Division of the DOE Office of Basic Energy Sciences under US DOE Contract No. DE-AC05-00OR22725.

FundersFunder number
Department of Energy Office of Biological and Environmental ResearchKP1605010
Life Science Biomedical Technology Research
Ministry of Education in Saudi Arabia
NSF-MRI1228817
National Science FoundationDGE-1747453, DGE-1747453 Giolas-Harriot/Crandall-Cordero
National Institutes of HealthS10 OD012331
U.S. Department of EnergyDE-AC05-00OR22725
National Institute of General Medical SciencesP41GM111244
U.S. Department of EducationP200A150330
Division of Chemical, Bioengineering, Environmental, and Transport SystemsBAG-302208, 1605971
Office of Science
Basic Energy Sciences
Brookhaven National LaboratoryDESC0012704
University of Connecticut

    Fingerprint

    Dive into the research topics of 'Changes Experienced by Low-Concentration Lipid Bicelles as a Function of Temperature'. Together they form a unique fingerprint.

    Cite this