Abstract
Purpose: Cationic lipid-coated gold nanoparticles were developed for efficient intracellular delivery of therapeutic siRNA. Methods: Particle formation was characterized by UV-visible spectroscopy, atomic force microscopy, and dynamic light scattering analysis. Cellular uptake, gene silencing effect, and cytotoxicity were investigated in multiple human cancer cell lines. Results: Nanoparticles had a spherical nanostructure with highly cationic surface charge and could form stable nanosized polyelectrolyte complexes with siRNA via electrostatic interactions; complexes exhibited efficient intracellular uptake and significant gene silencing effect with markedly low cytotoxicity compared to the widely used polycationic carrier, linear polyethyleneimine. Conclusions: We demonstrated that cationic lipid-coated gold nanoparticles could be widely utilized as efficient and safe siRNA nanocarriers for diverse therapeutic and diagnostic applications.
| Original language | English |
|---|---|
| Pages (from-to) | 362-374 |
| Number of pages | 13 |
| Journal | Pharmaceutical Research: An Official Journal of the American Association of Pharmaceutical Scientists |
| Volume | 29 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2012 |
| Externally published | Yes |
Funding
The late Professor Tae Gwan Park supervised the overall work as a principal investigator. All coauthors deeply appreciate his invaluable contribution and educational efforts. This research was supported by the Ministry for Health, Welfare and Family Affairs, and the World Class University project, Basic Science Research Program (2010–0027955) from the Ministry of Education, Science and Technology, Republic of Korea.
| Funders | Funder number |
|---|---|
| Ministry of Health and Welfare | 2010–0027955 |
| Ministry of Education, Science and Technology |
Keywords
- Cancer therapy
- Cationic lipid
- Delivery system
- Gold nanoparticle
- siRNA
