Carbon nanofiber-based electrode interface to microbial biofilms

T. E. McKnight; A. V. Melechko; M. A. Guillorn; V. I. Merkulov; J. T. Fleming; D. Hensley; E. Hullander; J. McPherson; D. Nivens; G. S. Sayler; D. H. Lowndes; M. L. Simpson

Carbon nanofiber-based electrode interface to microbial biofilms

T. E. McKnight, A. V. Melechko, M. A. Guillorn, V. I. Merkulov, J. T. Fleming, D. Hensley, E. Hullander, J. McPherson, D. Nivens, G. S. Sayler, D. H. Lowndes, M. L. Simpson

Nanofabrication Research Laboratory

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We report the fabrication and characterization of a carbon nanofiber-based electrochemical interface to microbial biofilms. Interdigitated electrode systems, comprised of 2 μm wide electrodes populated with forests of vertically-aligned carbon nanofibers, were developed to provide scaffolding, electrical stimuli, and measurement of metabolites around and within a suspended bacterial biofilm matrix. The presence of nanofiber forests upon these electrodes provides large, electrochemically active surface areas, resulting in high current densities during electroanalyses. The vertical aspect of nanofibers provides basis for interrogation around and within biofilm matrices, via potential penetration of nanofiber elements into the biomass. Fibered electrodes were characterized electrochemically using standard analytes and were employed as scaffolding to grow biofilms of various e. coli strains featuring bioluminescent gene-reporting constructs.

Original language	English
Title of host publication	2003 Nanotechnology Conference and Trade Show - Nanotech 2003
Editors	M. Laudon, B. Romanowicz
Pages	293-296
Number of pages	4
State	Published - 2003
Event	2003 Nanotechnology Conference and Trade Show - Nanotech 2003 - San Francisco, CA, United States Duration: Feb 23 2003 → Feb 27 2003

Publication series

Name	2003 Nanotechnology Conference and Trade Show - Nanotech 2003
Volume	3

Conference

Conference	2003 Nanotechnology Conference and Trade Show - Nanotech 2003
Country/Territory	United States
City	San Francisco, CA
Period	02/23/03 → 02/27/03

Keywords

Biofilm
Biosensor
Carbon nanofiber
Electrochemistry
Microelectrode

Cite this

McKnight, T. E., Melechko, A. V., Guillorn, M. A., Merkulov, V. I., Fleming, J. T., Hensley, D., Hullander, E., McPherson, J., Nivens, D., Sayler, G. S., Lowndes, D. H., & Simpson, M. L. (2003). Carbon nanofiber-based electrode interface to microbial biofilms. In M. Laudon, & B. Romanowicz (Eds.), 2003 Nanotechnology Conference and Trade Show - Nanotech 2003 (pp. 293-296). (2003 Nanotechnology Conference and Trade Show - Nanotech 2003; Vol. 3).

@inproceedings{32b8ab948321498f9a1fad3e340fda24,

title = "Carbon nanofiber-based electrode interface to microbial biofilms",

abstract = "We report the fabrication and characterization of a carbon nanofiber-based electrochemical interface to microbial biofilms. Interdigitated electrode systems, comprised of 2 μm wide electrodes populated with forests of vertically-aligned carbon nanofibers, were developed to provide scaffolding, electrical stimuli, and measurement of metabolites around and within a suspended bacterial biofilm matrix. The presence of nanofiber forests upon these electrodes provides large, electrochemically active surface areas, resulting in high current densities during electroanalyses. The vertical aspect of nanofibers provides basis for interrogation around and within biofilm matrices, via potential penetration of nanofiber elements into the biomass. Fibered electrodes were characterized electrochemically using standard analytes and were employed as scaffolding to grow biofilms of various e. coli strains featuring bioluminescent gene-reporting constructs.",

keywords = "Biofilm, Biosensor, Carbon nanofiber, Electrochemistry, Microelectrode",

author = "McKnight, {T. E.} and Melechko, {A. V.} and Guillorn, {M. A.} and Merkulov, {V. I.} and Fleming, {J. T.} and D. Hensley and E. Hullander and J. McPherson and D. Nivens and Sayler, {G. S.} and Lowndes, {D. H.} and Simpson, {M. L.}",

year = "2003",

language = "English",

isbn = "0972842209",

series = "2003 Nanotechnology Conference and Trade Show - Nanotech 2003",

pages = "293--296",

editor = "M. Laudon and B. Romanowicz",

booktitle = "2003 Nanotechnology Conference and Trade Show - Nanotech 2003",

note = "2003 Nanotechnology Conference and Trade Show - Nanotech 2003 ; Conference date: 23-02-2003 Through 27-02-2003",

}

McKnight, TE, Melechko, AV, Guillorn, MA, Merkulov, VI, Fleming, JT, Hensley, D, Hullander, E, McPherson, J, Nivens, D, Sayler, GS, Lowndes, DH & Simpson, ML 2003, Carbon nanofiber-based electrode interface to microbial biofilms. in M Laudon & B Romanowicz (eds), 2003 Nanotechnology Conference and Trade Show - Nanotech 2003. 2003 Nanotechnology Conference and Trade Show - Nanotech 2003, vol. 3, pp. 293-296, 2003 Nanotechnology Conference and Trade Show - Nanotech 2003, San Francisco, CA, United States, 02/23/03.

Carbon nanofiber-based electrode interface to microbial biofilms. / McKnight, T. E.; Melechko, A. V.; Guillorn, M. A. et al.
2003 Nanotechnology Conference and Trade Show - Nanotech 2003. ed. / M. Laudon; B. Romanowicz. 2003. p. 293-296 (2003 Nanotechnology Conference and Trade Show - Nanotech 2003; Vol. 3).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Carbon nanofiber-based electrode interface to microbial biofilms

AU - McKnight, T. E.

AU - Melechko, A. V.

AU - Guillorn, M. A.

AU - Merkulov, V. I.

AU - Fleming, J. T.

AU - Hensley, D.

AU - Hullander, E.

AU - McPherson, J.

AU - Nivens, D.

AU - Sayler, G. S.

AU - Lowndes, D. H.

AU - Simpson, M. L.

PY - 2003

Y1 - 2003

N2 - We report the fabrication and characterization of a carbon nanofiber-based electrochemical interface to microbial biofilms. Interdigitated electrode systems, comprised of 2 μm wide electrodes populated with forests of vertically-aligned carbon nanofibers, were developed to provide scaffolding, electrical stimuli, and measurement of metabolites around and within a suspended bacterial biofilm matrix. The presence of nanofiber forests upon these electrodes provides large, electrochemically active surface areas, resulting in high current densities during electroanalyses. The vertical aspect of nanofibers provides basis for interrogation around and within biofilm matrices, via potential penetration of nanofiber elements into the biomass. Fibered electrodes were characterized electrochemically using standard analytes and were employed as scaffolding to grow biofilms of various e. coli strains featuring bioluminescent gene-reporting constructs.

AB - We report the fabrication and characterization of a carbon nanofiber-based electrochemical interface to microbial biofilms. Interdigitated electrode systems, comprised of 2 μm wide electrodes populated with forests of vertically-aligned carbon nanofibers, were developed to provide scaffolding, electrical stimuli, and measurement of metabolites around and within a suspended bacterial biofilm matrix. The presence of nanofiber forests upon these electrodes provides large, electrochemically active surface areas, resulting in high current densities during electroanalyses. The vertical aspect of nanofibers provides basis for interrogation around and within biofilm matrices, via potential penetration of nanofiber elements into the biomass. Fibered electrodes were characterized electrochemically using standard analytes and were employed as scaffolding to grow biofilms of various e. coli strains featuring bioluminescent gene-reporting constructs.

KW - Biofilm

KW - Biosensor

KW - Carbon nanofiber

KW - Electrochemistry

KW - Microelectrode

UR - http://www.scopus.com/inward/record.url?scp=6344262098&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:6344262098

SN - 0972842209

SN - 9780972842204

T3 - 2003 Nanotechnology Conference and Trade Show - Nanotech 2003

SP - 293

EP - 296

BT - 2003 Nanotechnology Conference and Trade Show - Nanotech 2003

A2 - Laudon, M.

A2 - Romanowicz, B.

T2 - 2003 Nanotechnology Conference and Trade Show - Nanotech 2003

Y2 - 23 February 2003 through 27 February 2003

ER -

Carbon nanofiber-based electrode interface to microbial biofilms

Abstract

Publication series

Conference

Keywords

Other files and links

Fingerprint

Cite this