Calibration of atomic force microscope tips using biomolecules

T. Thundat; X. Y. Zheng; S. L. Sharp; D. P. Allison; R. J. Warmack; D. C. Joy; T. L. Ferrell; R. Balhorn; J. K.H. Horber

Calibration of atomic force microscope tips using biomolecules

T. Thundat, X. Y. Zheng, S. L. Sharp, D. P. Allison, R. J. Warmack, D. C. Joy, T. L. Ferrell, R. Balhorn, J. K.H. Horber

Sensors and Electronics

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

Atomic force microscope (AFM) images of surfaces and samples mounted on substrates are subject to artifacts such as broadening of structures and ghost images of tips due to the finite size and shape of the contacting probe. Therefore, knowledge of the radius of the AFM probe tip is essential for the interpretation of images. We have deduced the shape of the AFM tip by imaging cylindrical biological molecules of various diameters such as deoxyribonucleic acid (DNA), tobacco mosaic virus (TMV), tobacco etch virus (TEV) and bacteriophage M-13 (M-13). Using a paraboloidal tip model and numerically solving equations of contact, the curvatures of the tip and lithographically sharpened tip were ascertained.

Original language	English
Pages (from-to)	903-910
Number of pages	8
Journal	Scanning Microscopy
Volume	6
Issue number	4
State	Published - Dec 1992

Cite this

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title = "Calibration of atomic force microscope tips using biomolecules",

abstract = "Atomic force microscope (AFM) images of surfaces and samples mounted on substrates are subject to artifacts such as broadening of structures and ghost images of tips due to the finite size and shape of the contacting probe. Therefore, knowledge of the radius of the AFM probe tip is essential for the interpretation of images. We have deduced the shape of the AFM tip by imaging cylindrical biological molecules of various diameters such as deoxyribonucleic acid (DNA), tobacco mosaic virus (TMV), tobacco etch virus (TEV) and bacteriophage M-13 (M-13). Using a paraboloidal tip model and numerically solving equations of contact, the curvatures of the tip and lithographically sharpened tip were ascertained.",

author = "T. Thundat and Zheng, {X. Y.} and Sharp, {S. L.} and Allison, {D. P.} and Warmack, {R. J.} and Joy, {D. C.} and Ferrell, {T. L.} and R. Balhorn and Horber, {J. K.H.}",

year = "1992",

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language = "English",

volume = "6",

pages = "903--910",

journal = "Scanning Microscopy",

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T1 - Calibration of atomic force microscope tips using biomolecules

AU - Thundat, T.

AU - Zheng, X. Y.

AU - Sharp, S. L.

AU - Allison, D. P.

AU - Warmack, R. J.

AU - Joy, D. C.

AU - Ferrell, T. L.

AU - Balhorn, R.

AU - Horber, J. K.H.

PY - 1992/12

Y1 - 1992/12

N2 - Atomic force microscope (AFM) images of surfaces and samples mounted on substrates are subject to artifacts such as broadening of structures and ghost images of tips due to the finite size and shape of the contacting probe. Therefore, knowledge of the radius of the AFM probe tip is essential for the interpretation of images. We have deduced the shape of the AFM tip by imaging cylindrical biological molecules of various diameters such as deoxyribonucleic acid (DNA), tobacco mosaic virus (TMV), tobacco etch virus (TEV) and bacteriophage M-13 (M-13). Using a paraboloidal tip model and numerically solving equations of contact, the curvatures of the tip and lithographically sharpened tip were ascertained.

AB - Atomic force microscope (AFM) images of surfaces and samples mounted on substrates are subject to artifacts such as broadening of structures and ghost images of tips due to the finite size and shape of the contacting probe. Therefore, knowledge of the radius of the AFM probe tip is essential for the interpretation of images. We have deduced the shape of the AFM tip by imaging cylindrical biological molecules of various diameters such as deoxyribonucleic acid (DNA), tobacco mosaic virus (TMV), tobacco etch virus (TEV) and bacteriophage M-13 (M-13). Using a paraboloidal tip model and numerically solving equations of contact, the curvatures of the tip and lithographically sharpened tip were ascertained.

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M3 - Article

AN - SCOPUS:0027052097

SN - 0891-7035

VL - 6

SP - 903

EP - 910

JO - Scanning Microscopy

JF - Scanning Microscopy

IS - 4

ER -

Calibration of atomic force microscope tips using biomolecules

Abstract

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