Bread wheat with enhanced grain carotenoid content: a novel option for wheat biofortification

Asish Kumar Padhy; Achla Sharma; Himanshu Sharma; Ruchika Rajput; Ashutosh Pandey; Puja Srivastava; Satinder Kaur; Harinderjit Kaur; Satinder Singh; Lenika Kashyap; Gurvinder Singh Mavi; Jaspal Kaur; Virinder Singh Sohu; Parveen Chhuneja; Navtej Singh Bains

doi:10.1007/s11032-022-01338-0

Bread wheat with enhanced grain carotenoid content: a novel option for wheat biofortification

Asish Kumar Padhy
, Achla Sharma
, Himanshu Sharma
, Ruchika Rajput
, Ashutosh Pandey
, Puja Srivastava
, Satinder Kaur
, Harinderjit Kaur
, Satinder Singh
, Lenika Kashyap
, Gurvinder Singh Mavi
, Jaspal Kaur
, Virinder Singh Sohu
, Parveen Chhuneja
, Navtej Singh Bains

Synthetic Biology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Colored wheat has piqued the interest of breeders and consumers alike. The chromosomal segment from 7E of Thinopyrum ponticum, which carries a leaf rust resistant gene, Lr19, has been rarely employed in wheat breeding operations due to its association with the Y gene, which gives a yellow tint to the flour. By prioritizing nutritional content over color preferences, consumer acceptance has undergone a paradigm change. Through marker-assisted backcross breeding, we introduced an alien segment harboring the Y (PsyE1) gene into a high yielding commercial bread wheat (HD 2967) background to generate rust resistant carotenoid biofortified bread wheat. Agro-morphological characterization was also performed on a subset of developed 70 lines having enhanced grain carotene content. In the introgression lines, carotenoid profiling using HPLC analysis demonstrated a considerable increase in β-carotene levels (up to 12 ppm). Thus, the developed germplasm caters the threat to nutritional security and can be utilized to produce carotenoid fortified wheat.

Original language	English
Article number	67
Journal	Molecular Breeding
Volume	42
Issue number	11
DOIs	https://doi.org/10.1007/s11032-022-01338-0
State	Published - Nov 2022

Funding

The authors from Punjab Agricultural University are grateful to field/lab staff (Gagandeep Singh and Khushbir Kaur) for timely help in raising/maintenance of the crop and assistance in lab work. The authors from NIPGR are thankful to Sh. Sobha Ram for assistance in HPLC analysis. The Department of Science and Technology, Government of India provided financial support under project, “Addressing Food Security through Nutritionally Enriched Improved Cultivars and Technologies for Swasth Bharat” under “Promotion of University Research and Scientific Excellence (PURSE) Grant” Grant number SR/PURSE Phase 2/25(G), 28.09.2017.

Keywords

Biofortification
Bread wheat
Carotenoid
Phytoene synthase
PsyE1 (Y gene)

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10.1007/s11032-022-01338-0

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Padhy, A. K., Sharma, A., Sharma, H., Rajput, R., Pandey, A., Srivastava, P., Kaur, S., Kaur, H., Singh, S., Kashyap, L., Mavi, G. S., Kaur, J., Sohu, V. S., Chhuneja, P., & Bains, N. S. (2022). Bread wheat with enhanced grain carotenoid content: a novel option for wheat biofortification. Molecular Breeding, 42(11), Article 67. https://doi.org/10.1007/s11032-022-01338-0

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abstract = "Colored wheat has piqued the interest of breeders and consumers alike. The chromosomal segment from 7E of Thinopyrum ponticum, which carries a leaf rust resistant gene, Lr19, has been rarely employed in wheat breeding operations due to its association with the Y gene, which gives a yellow tint to the flour. By prioritizing nutritional content over color preferences, consumer acceptance has undergone a paradigm change. Through marker-assisted backcross breeding, we introduced an alien segment harboring the Y (PsyE1) gene into a high yielding commercial bread wheat (HD 2967) background to generate rust resistant carotenoid biofortified bread wheat. Agro-morphological characterization was also performed on a subset of developed 70 lines having enhanced grain carotene content. In the introgression lines, carotenoid profiling using HPLC analysis demonstrated a considerable increase in β-carotene levels (up to 12 ppm). Thus, the developed germplasm caters the threat to nutritional security and can be utilized to produce carotenoid fortified wheat.",

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AU - Kaur, Satinder

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AU - Singh, Satinder

AU - Kashyap, Lenika

AU - Mavi, Gurvinder Singh

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AU - Sohu, Virinder Singh

AU - Chhuneja, Parveen

AU - Bains, Navtej Singh

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AB - Colored wheat has piqued the interest of breeders and consumers alike. The chromosomal segment from 7E of Thinopyrum ponticum, which carries a leaf rust resistant gene, Lr19, has been rarely employed in wheat breeding operations due to its association with the Y gene, which gives a yellow tint to the flour. By prioritizing nutritional content over color preferences, consumer acceptance has undergone a paradigm change. Through marker-assisted backcross breeding, we introduced an alien segment harboring the Y (PsyE1) gene into a high yielding commercial bread wheat (HD 2967) background to generate rust resistant carotenoid biofortified bread wheat. Agro-morphological characterization was also performed on a subset of developed 70 lines having enhanced grain carotene content. In the introgression lines, carotenoid profiling using HPLC analysis demonstrated a considerable increase in β-carotene levels (up to 12 ppm). Thus, the developed germplasm caters the threat to nutritional security and can be utilized to produce carotenoid fortified wheat.

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KW - Phytoene synthase

KW - PsyE1 (Y gene)

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ER -

Bread wheat with enhanced grain carotenoid content: a novel option for wheat biofortification

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