Bioluminescent-bioreporter integrated circuits form novel whole-cell biosensors

Michael L. Simpson; Gary S. Sayler; Bruce M. Applegate; Steven Ripp; David E. Nivens; Michael J. Paulus; Gerald E. Jellison

doi:10.1016/S0167-7799(98)01199-8

Bioluminescent-bioreporter integrated circuits form novel whole-cell biosensors

Michael L. Simpson, Gary S. Sayler, Bruce M. Applegate, Steven Ripp, David E. Nivens, Michael J. Paulus, Gerald E. Jellison

Research output: Contribution to journal › Article › peer-review

110 Scopus citations

Abstract

The bioluminescent-bioreporter integrated circuit represents a new advance in the development of whole-cell biosensors, consisting of a genetically engineered bioreporter organism interfaced with an integrated circuit. The bioreporter is engineered to luminesce when a targeted substance is encountered, with the circuit being designed to detect this luminescence, process the signal and communicate the results. The bioreporters are thus available to the circuit designer as another component, analogous to a transistor or a resistor, albeit a living entity. The concept has been demonstrated using Pseudomonas putida TVA8 to sense the environmental pollutant toluene.

Original language	English
Pages (from-to)	332-338
Number of pages	7
Journal	Trends in Biotechnology
Volume	16
Issue number	8
DOIs	https://doi.org/10.1016/S0167-7799(98)01199-8
State	Published - Dec 1 1998

Funding

The authors are partially supported by US DOE grant #DE-FG05-94ER61870 from the Office of Biological and Environmental Research and partially performed at the Oak Ridge National Laboratory. S. Ripp was supported by an appointment to the Alexander Hollaender Distinguished Postdoctoral Fellowship Program sponsored by the US Department of Energy, Office of Health and Environmental Research and administered by the Oak Ridge Institute for Science and Education.

Access to Document

10.1016/S0167-7799(98)01199-8

Cite this

@article{cf80196d1fdd49dfa4281f706033198c,

title = "Bioluminescent-bioreporter integrated circuits form novel whole-cell biosensors",

abstract = "The bioluminescent-bioreporter integrated circuit represents a new advance in the development of whole-cell biosensors, consisting of a genetically engineered bioreporter organism interfaced with an integrated circuit. The bioreporter is engineered to luminesce when a targeted substance is encountered, with the circuit being designed to detect this luminescence, process the signal and communicate the results. The bioreporters are thus available to the circuit designer as another component, analogous to a transistor or a resistor, albeit a living entity. The concept has been demonstrated using Pseudomonas putida TVA8 to sense the environmental pollutant toluene.",

author = "Simpson, {Michael L.} and Sayler, {Gary S.} and Applegate, {Bruce M.} and Steven Ripp and Nivens, {David E.} and Paulus, {Michael J.} and Jellison, {Gerald E.}",

year = "1998",

month = dec,

day = "1",

doi = "10.1016/S0167-7799(98)01199-8",

language = "English",

volume = "16",

pages = "332--338",

journal = "Trends in Biotechnology",

issn = "0167-7799",

publisher = "Cell Press",

number = "8",

}

TY - JOUR

T1 - Bioluminescent-bioreporter integrated circuits form novel whole-cell biosensors

AU - Simpson, Michael L.

AU - Sayler, Gary S.

AU - Applegate, Bruce M.

AU - Ripp, Steven

AU - Nivens, David E.

AU - Paulus, Michael J.

AU - Jellison, Gerald E.

PY - 1998/12/1

Y1 - 1998/12/1

N2 - The bioluminescent-bioreporter integrated circuit represents a new advance in the development of whole-cell biosensors, consisting of a genetically engineered bioreporter organism interfaced with an integrated circuit. The bioreporter is engineered to luminesce when a targeted substance is encountered, with the circuit being designed to detect this luminescence, process the signal and communicate the results. The bioreporters are thus available to the circuit designer as another component, analogous to a transistor or a resistor, albeit a living entity. The concept has been demonstrated using Pseudomonas putida TVA8 to sense the environmental pollutant toluene.

AB - The bioluminescent-bioreporter integrated circuit represents a new advance in the development of whole-cell biosensors, consisting of a genetically engineered bioreporter organism interfaced with an integrated circuit. The bioreporter is engineered to luminesce when a targeted substance is encountered, with the circuit being designed to detect this luminescence, process the signal and communicate the results. The bioreporters are thus available to the circuit designer as another component, analogous to a transistor or a resistor, albeit a living entity. The concept has been demonstrated using Pseudomonas putida TVA8 to sense the environmental pollutant toluene.

UR - http://www.scopus.com/inward/record.url?scp=0032404396&partnerID=8YFLogxK

U2 - 10.1016/S0167-7799(98)01199-8

DO - 10.1016/S0167-7799(98)01199-8

M3 - Article

AN - SCOPUS:0032404396

SN - 0167-7799

VL - 16

SP - 332

EP - 338

JO - Trends in Biotechnology

JF - Trends in Biotechnology

IS - 8

ER -

Bioluminescent-bioreporter integrated circuits form novel whole-cell biosensors

Abstract

Funding

Access to Document

Other files and links

Fingerprint

Cite this