Bioluminescent-bioreporter integrated circuits form novel whole-cell biosensors

Michael L. Simpson; Gary S. Sayler; Bruce M. Applegate; Steven Ripp; David E. Nivens; Michael J. Paulus; Gerald E. Jellison

doi:10.1016/S0167-7799(98)01199-8

Bioluminescent-bioreporter integrated circuits form novel whole-cell biosensors

Michael L. Simpson, Gary S. Sayler, Bruce M. Applegate, Steven Ripp, David E. Nivens, Michael J. Paulus, Gerald E. Jellison

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110 Scopus citations

Abstract

The bioluminescent-bioreporter integrated circuit represents a new advance in the development of whole-cell biosensors, consisting of a genetically engineered bioreporter organism interfaced with an integrated circuit. The bioreporter is engineered to luminesce when a targeted substance is encountered, with the circuit being designed to detect this luminescence, process the signal and communicate the results. The bioreporters are thus available to the circuit designer as another component, analogous to a transistor or a resistor, albeit a living entity. The concept has been demonstrated using Pseudomonas putida TVA8 to sense the environmental pollutant toluene.

Original language	English
Pages (from-to)	332-338
Number of pages	7
Journal	Trends in Biotechnology
Volume	16
Issue number	8
DOIs	https://doi.org/10.1016/S0167-7799(98)01199-8
State	Published - Dec 1 1998

Funding

The authors are partially supported by US DOE grant #DE-FG05-94ER61870 from the Office of Biological and Environmental Research and partially performed at the Oak Ridge National Laboratory. S. Ripp was supported by an appointment to the Alexander Hollaender Distinguished Postdoctoral Fellowship Program sponsored by the US Department of Energy, Office of Health and Environmental Research and administered by the Oak Ridge Institute for Science and Education.

Funders	Funder number
Office of Health and Environmental Research
U.S. Department of Energy	-FG05-94ER61870
Biological and Environmental Research
Oak Ridge National Laboratory
Oak Ridge Institute for Science and Education

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abstract = "The bioluminescent-bioreporter integrated circuit represents a new advance in the development of whole-cell biosensors, consisting of a genetically engineered bioreporter organism interfaced with an integrated circuit. The bioreporter is engineered to luminesce when a targeted substance is encountered, with the circuit being designed to detect this luminescence, process the signal and communicate the results. The bioreporters are thus available to the circuit designer as another component, analogous to a transistor or a resistor, albeit a living entity. The concept has been demonstrated using Pseudomonas putida TVA8 to sense the environmental pollutant toluene.",

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AU - Paulus, Michael J.

AU - Jellison, Gerald E.

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Bioluminescent-bioreporter integrated circuits form novel whole-cell biosensors

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