Bioelectronic Recordings of Cardiomyocytes with Accumulation Mode Electrolyte Gated Organic Field Effect Transistors

Adrica Kyndiah; Francesca Leonardi; Carolina Tarantino; Tobias Cramer; Ruben Millan-Solsona; Elena Garreta; Núria Montserrat; Marta Mas-Torrent; Gabriel Gomila

doi:10.1016/j.bios.2019.111844

Bioelectronic Recordings of Cardiomyocytes with Accumulation Mode Electrolyte Gated Organic Field Effect Transistors

Adrica Kyndiah
, Francesca Leonardi
, Carolina Tarantino
, Tobias Cramer
, Ruben Millan-Solsona
, Elena Garreta
, Núria Montserrat
, Marta Mas-Torrent
, Gabriel Gomila

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Organic electronic materials offer an untapped potential for novel tools for low-invasive electrophysiological recording and stimulation devices. Such materials combine semiconducting properties with tailored surface chemistry, elastic mechanical properties and chemical stability in water. In this work, we investigate solution processed Electrolyte Gated Organic Field Effect Transistors (EGOFETs) based on a small molecule semiconductor. We demonstrate that EGOFETs based on a blend of soluble organic semiconductor 2,8-Difluoro-5,11-bis(triethylsilylethynyl)anthradithiophene (diF-TES-ADT) combined with an insulating polymer show excellent sensitivity and long-term recording under electrophysiological applications. Our devices can stably record the extracellular potential of human pluripotent stem cell derived cardiomyocyte cells (hPSCs-CMs) for several weeks. In addition, cytotoxicity tests of pharmaceutical drugs, such as Norepinephrine and Verapamil was achieved with excellent sensitivity. This work demonstrates that organic transistors based on organic blends are excellent bioelectronics transducer for extracellular electrical recording of excitable cells and tissues thus providing a valid alternative to electrochemical transistors.

Original language	English
Article number	111844
Journal	Biosensors and Bioelectronics
Volume	150
DOIs	https://doi.org/10.1016/j.bios.2019.111844
State	Published - Feb 15 2020
Externally published	Yes

Funding

This work has been partially supported by the BEST Postdoctoral Programme funded by the European Commission under Horizon 2020's Marie Curie Sklodowska-Curie Actions COFUND scheme (GA 712754) and the Severo Ochoa programme of the Spanish Ministry of Science and Competitiveness (SEV-2014-0425 (2015–2019) and SEV-2015-0496) and the BORGES project (Marie Curie Skłodowska European Training Network (MSCA-ITN-ETN)) under the GrantAgreement (GA) No: 813863. G. G. acknowledges support from an ICREA Academia award from the Generalitat de Catalunya and from the Agencia Estatal de Investigación (Nanoelectrophys project, TEC2016-79156-P). The authors also thank the DGI (Spain) project FANCY CTQ2016-80030-R, the Generalitat de Catalunya (2017-SGR-918) and the Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN). Dr. F. Leonardi gratefully acknowledges the “Juan de la Cierva” programme. The authors thank S. Ricci for her help on the samples preparation. This work has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (StG-2014-640525_REGMAMKID to C.T. and N.M.). NM has received funding from the Spanish Ministry of Economy and Competitiveness/FEDER (SAF2015-72617-EXP and SAF2017-89782-), the Generalitat de Catalunya and CERCA programme (2017 SGR 1306), Asociación Española contra el Cáncer (LABAE16006 to N.M.) and by CardioCel (TerCel, Instituto de Salud Carlos III). IBEC is the recipient of a Severo Ochoa Award of Excellence from MINECO. This work has been partially supported by the BEST Postdoctoral Programme funded by the European Commission under Horizon 2020's Marie Curie Sklodowska-Curie Actions COFUND scheme (GA 712754 ) and the Severo Ochoa programme of the Spanish Ministry of Science and Competitiveness ( SEV-2014-0425 ( 2015–2019 ) and SEV-2015-0496 ) and the BORGES project (Marie Curie Skłodowska European Training Network (MSCA-ITN-ETN)) under the GrantAgreement ( GA ) No: 813863 . G. G. acknowledges support from an ICREA Academia award from the Generalitat de Catalunya and from the Agencia Estatal de Investigación (Nanoelectrophys project, TEC2016-79156-P). The authors also thank the DGI (Spain) project FANCY CTQ2016-80030-R, the Generalitat de Catalunya (2017-SGR-918) and the Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN). Dr. F. Leonardi gratefully acknowledges the “Juan de la Cierva” programme. The authors thank S. Ricci for her help on the samples preparation. This work has received funding from the European Research Council ( ERC ) under the European Union's Horizon 2020 research and innovation programme (StG-2014-640525_REGMAMKID to C.T. and N.M.). NM has received funding from the Spanish Ministry of Economy and Competitiveness /FEDER ( SAF2015-72617-EXP and SAF2017-89782- ), the Generalitat de Catalunya and CERCA programme (2017 SGR 1306 ), Asociación Española contra el Cáncer (LABAE16006 to N.M.) and by CardioCel (TerCel, Instituto de Salud Carlos III ). IBEC is the recipient of a Severo Ochoa Award of Excellence from MINECO .

Keywords

Bioelectronics
Cardiac cells
Organic electronics
Organic field effect transistors
Organic semiconducting blend

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10.1016/j.bios.2019.111844

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title = "Bioelectronic Recordings of Cardiomyocytes with Accumulation Mode Electrolyte Gated Organic Field Effect Transistors",

abstract = "Organic electronic materials offer an untapped potential for novel tools for low-invasive electrophysiological recording and stimulation devices. Such materials combine semiconducting properties with tailored surface chemistry, elastic mechanical properties and chemical stability in water. In this work, we investigate solution processed Electrolyte Gated Organic Field Effect Transistors (EGOFETs) based on a small molecule semiconductor. We demonstrate that EGOFETs based on a blend of soluble organic semiconductor 2,8-Difluoro-5,11-bis(triethylsilylethynyl)anthradithiophene (diF-TES-ADT) combined with an insulating polymer show excellent sensitivity and long-term recording under electrophysiological applications. Our devices can stably record the extracellular potential of human pluripotent stem cell derived cardiomyocyte cells (hPSCs-CMs) for several weeks. In addition, cytotoxicity tests of pharmaceutical drugs, such as Norepinephrine and Verapamil was achieved with excellent sensitivity. This work demonstrates that organic transistors based on organic blends are excellent bioelectronics transducer for extracellular electrical recording of excitable cells and tissues thus providing a valid alternative to electrochemical transistors.",

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AU - Millan-Solsona, Ruben

AU - Garreta, Elena

AU - Montserrat, Núria

AU - Mas-Torrent, Marta

AU - Gomila, Gabriel

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N2 - Organic electronic materials offer an untapped potential for novel tools for low-invasive electrophysiological recording and stimulation devices. Such materials combine semiconducting properties with tailored surface chemistry, elastic mechanical properties and chemical stability in water. In this work, we investigate solution processed Electrolyte Gated Organic Field Effect Transistors (EGOFETs) based on a small molecule semiconductor. We demonstrate that EGOFETs based on a blend of soluble organic semiconductor 2,8-Difluoro-5,11-bis(triethylsilylethynyl)anthradithiophene (diF-TES-ADT) combined with an insulating polymer show excellent sensitivity and long-term recording under electrophysiological applications. Our devices can stably record the extracellular potential of human pluripotent stem cell derived cardiomyocyte cells (hPSCs-CMs) for several weeks. In addition, cytotoxicity tests of pharmaceutical drugs, such as Norepinephrine and Verapamil was achieved with excellent sensitivity. This work demonstrates that organic transistors based on organic blends are excellent bioelectronics transducer for extracellular electrical recording of excitable cells and tissues thus providing a valid alternative to electrochemical transistors.

AB - Organic electronic materials offer an untapped potential for novel tools for low-invasive electrophysiological recording and stimulation devices. Such materials combine semiconducting properties with tailored surface chemistry, elastic mechanical properties and chemical stability in water. In this work, we investigate solution processed Electrolyte Gated Organic Field Effect Transistors (EGOFETs) based on a small molecule semiconductor. We demonstrate that EGOFETs based on a blend of soluble organic semiconductor 2,8-Difluoro-5,11-bis(triethylsilylethynyl)anthradithiophene (diF-TES-ADT) combined with an insulating polymer show excellent sensitivity and long-term recording under electrophysiological applications. Our devices can stably record the extracellular potential of human pluripotent stem cell derived cardiomyocyte cells (hPSCs-CMs) for several weeks. In addition, cytotoxicity tests of pharmaceutical drugs, such as Norepinephrine and Verapamil was achieved with excellent sensitivity. This work demonstrates that organic transistors based on organic blends are excellent bioelectronics transducer for extracellular electrical recording of excitable cells and tissues thus providing a valid alternative to electrochemical transistors.

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KW - Organic semiconducting blend

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Bioelectronic Recordings of Cardiomyocytes with Accumulation Mode Electrolyte Gated Organic Field Effect Transistors

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