Biodegradable polymeric microspheres with "open/closed" pores for sustained release of human growth hormone

Hong Kee Kim, Hyun Jung Chung, Tae Gwan Park

Research output: Contribution to journalArticlepeer-review

235 Scopus citations

Abstract

A new approach for attaining sustained release of protein is introduced, involving a pore-closing process of preformed porous PLGA microspheres. Highly porous biodegradable poly(d,l-lactic-co-glycolic acid) (PLGA) microspheres were fabricated by a single water-in-oil emulsion solvent evaporation technique using Pluronic F127 as an extractable porogen. Recombinant human growth hormone (rhGH) was incorporated into porous microspheres by a simple solution dipping method. For their controlled release, porous microspheres containing hGH were treated with water-miscible solvents in aqueous phase for production of pore-closed microspheres. These microspheres showed sustained release patterns over an extended period; however, the drug loading efficiency was extremely low. To overcome the drug loading problem, the pore-closing process was performed in an ethanol vapor phase using a fluidized bed reactor. The resultant pore-closed microspheres exhibited high protein loading amount as well as sustained rhGH release profiles. Also, the released rhGH exhibited structural integrity after the treatment.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalJournal of Controlled Release
Volume112
Issue number2
DOIs
StatePublished - May 15 2006
Externally publishedYes

Funding

This work was supported by the grants for the Ministry of Science and Technology and the Ministry of Commerce, Industry and Energy, Republic of Korea.

Keywords

  • Biodegradable
  • Fluidized bed reactor
  • Microspheres
  • PLGA
  • Porous
  • Protein delivery

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