Abstract
A new approach for attaining sustained release of protein is introduced, involving a pore-closing process of preformed porous PLGA microspheres. Highly porous biodegradable poly(d,l-lactic-co-glycolic acid) (PLGA) microspheres were fabricated by a single water-in-oil emulsion solvent evaporation technique using Pluronic F127 as an extractable porogen. Recombinant human growth hormone (rhGH) was incorporated into porous microspheres by a simple solution dipping method. For their controlled release, porous microspheres containing hGH were treated with water-miscible solvents in aqueous phase for production of pore-closed microspheres. These microspheres showed sustained release patterns over an extended period; however, the drug loading efficiency was extremely low. To overcome the drug loading problem, the pore-closing process was performed in an ethanol vapor phase using a fluidized bed reactor. The resultant pore-closed microspheres exhibited high protein loading amount as well as sustained rhGH release profiles. Also, the released rhGH exhibited structural integrity after the treatment.
Original language | English |
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Pages (from-to) | 167-174 |
Number of pages | 8 |
Journal | Journal of Controlled Release |
Volume | 112 |
Issue number | 2 |
DOIs | |
State | Published - May 15 2006 |
Externally published | Yes |
Funding
This work was supported by the grants for the Ministry of Science and Technology and the Ministry of Commerce, Industry and Energy, Republic of Korea.
Keywords
- Biodegradable
- Fluidized bed reactor
- Microspheres
- PLGA
- Porous
- Protein delivery