Abstract
The bioconversion of homogeneous linear catechyl lignin (C-lignin) to polyhydroxyalkanoates (PHA) was examined for the first time in this study. C-lignins from vanilla, euphorbia, and candlenut seed coats (denoted as C1, C2, and C3, respectively) varied in their molecular structures, which showed different molecular weight distributions, etherification degrees, and contents of hydroxyl groups. A notable amount of nonetherified catechol units existed within C1 and C2 lignins, and these catechol units were consumed during fermentation. These results suggested that the nonetherified catechol structure was readily converted by Pseudomonas putida KT2440. Since the weight-average molecular weight of C2 raw lignin was 26.7% lower than that of C1, the bioconversion performance of C2 lignin was more outstanding. The P. putida KT2440 cell amount reached the maximum of 9.3 × 107 CFU/mL in the C2 medium, which was 37.9 and 82.4% higher than that in the C1 and C3 medium, respectively. Accordingly, PHA concentration reached 137 mg/L within the C2 medium, which was 41.2 and 149.1% higher than the C1 and C3 medium, respectively. Overall, C-lignin, with a nonetherified catechol structure and low molecular weight, benefits its microbial conversion significantly.
| Original language | English |
|---|---|
| Pages (from-to) | 3996-4004 |
| Number of pages | 9 |
| Journal | Biomacromolecules |
| Volume | 24 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 11 2023 |
Funding
Z-M.Z. acknowledges the Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region, China (NJYT23081), the National Natural Science Foundation of China (21706136), and the China Scholarship Council (CSC) for their support. This work was partially (A.J.R., Y.P., and F.C.) supported by the Center for Bioenergy Innovation (CBI), a US Department of Energy (DOE) Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle, LLC under Contract DE-AC05-00OR22725 with the U.S. DOE. The views and opinions of the authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, expressed or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights.
| Funders | Funder number |
|---|---|
| Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region | NJYT23081 |
| U.S. Department of Energy | |
| Office of Science | |
| Biological and Environmental Research | |
| Oak Ridge National Laboratory | |
| Center for Bioenergy Innovation | |
| UT-Battelle | DE-AC05-00OR22725 |
| National Natural Science Foundation of China | 21706136 |
| China Scholarship Council |