Abstract
A robust biocatalytic yarn with controllable liquid transport properties is created by coating thin layers of chitosan containing catalase onto a cellulosic yarn. The resulting material integrates enzyme catalytic functionality with protective coating properties of chitosan and structural functionality of the textile. Mild immobilization conditions and good affinity between the two polysaccharides minimize enzyme inactivation during the preparation steps and prevent enzyme from leaching during peroxide decomposition testing and washing, providing a novel and versatile enzyme immobilization strategy. The catalytic efficiency of enzymes in a reaction containing solid, liquid, and gas phases is facilitated when dissolved enzyme substrate is transported by liquid flowing through the coated textile structure. The flow-through configuration decomposes at least two times more peroxide in a twenty-times smaller reaction zone volume compared to a stirred tank configuration. Liquid transport through the yarn and liquid spatial distribution within the yarn are investigated by in situ neutron radiography and neutron computed tomography, revealing a constrained wicking mechanism that benefits biocatalytic yarn performance. This new class of sustainable and flexible biocatalytic textile matrices has beneficial multifunctional properties, not previously described, that are applicable for numerous small- and large-scale applications including controlled flow reactors and reactive filtration.
| Original language | English |
|---|---|
| Article number | 2002104 |
| Journal | Advanced Materials Interfaces |
| Volume | 8 |
| Issue number | 7 |
| DOIs | |
| State | Published - Apr 9 2021 |
Funding
This work was supported by Wilson College of Textiles at North Carolina State University. The authors appreciate Novozymes for contributing catalase (Terminox Ultra) for this study and appreciate Chuck Money at the NC State University Analytical Instrumentation Facility (AIF) for help with SEM instrumentation and sample preparation. Neutron radiography and neutron computed tomography experiments (CG‐1D Neutron Imaging Proof‐of‐Principle Proposal IPTS‐24561.1) used resources at the High Flux Isotope Reactor, a DOE Office of Science User Facility operated by the Oak Ridge National Laboratory. The authors also appreciate Erik Stringfellow for helpful discussions on neutron experiment setup. This manuscript has been authored by UT‐Battelle, LLC under Contract No. DE‐AC05‐00OR22725 with the U.S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non‐exclusive, paid‐up, irrevocable, worldwide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan ( http://energy.gov/downloads/doe‐public‐access‐plan ). This work was supported by Wilson College of Textiles at North Carolina State University. The authors appreciate Novozymes for contributing catalase (Terminox Ultra) for this study and appreciate Chuck Money at the NC State University Analytical Instrumentation Facility (AIF) for help with SEM instrumentation and sample preparation. Neutron radiography and neutron computed tomography experiments (CG-1D Neutron Imaging Proof-of-Principle Proposal IPTS-24561.1) used resources at the High Flux Isotope Reactor, a DOE Office of Science User Facility operated by the Oak Ridge National Laboratory. The authors also appreciate Erik Stringfellow for helpful discussions on neutron experiment setup. This manuscript has been authored by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the U.S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan).
| Funders | Funder number |
|---|---|
| DOE Public Access Plan | |
| United States Government | |
| Wilson College of Textiles at North Carolina State University | |
| U.S. Department of Energy | |
| Office of Science | |
| Oak Ridge National Laboratory | DE-AC05-00OR22725 |
| North Carolina State University |
Keywords
- biocatalytic yarn
- constrained liquid transport
- enzyme immobilization
- flexible flow-through reactors
- textile