Bio-stabilized AuNPs as a reliable fluorescence sensing probe for Hg2+: application for environmental water sample

Shubhangi Mane-Gavade; Supriya A. Patil; Vidhya Jadhav; Akshata Pattanshetti; Sandip Nipane; Xiao Ying Yu; Deok Kee Kim; Sandip Sabale

doi:10.1007/s43630-025-00718-7

Bio-stabilized AuNPs as a reliable fluorescence sensing probe for Hg²⁺: application for environmental water sample

Shubhangi Mane-Gavade, Supriya A. Patil, Vidhya Jadhav, Akshata Pattanshetti, Sandip Nipane, Xiao Ying Yu, Deok Kee Kim, Sandip Sabale

Advanced Nuclear Materials

Research output: Contribution to journal › Article › peer-review

Abstract

Gold nanoparticles (AuNPs) have gained attention due to their unique optical and electronic properties. Their exceptional stability, high conductivity, and strong light interaction also make them ideal for sensing applications. In this study, we developed a green microwave-assisted approach for synthesizing uniform (~ 10 nm) AuNPs using Acacia concinna fruit extract as a bio-stabilizing agent. Synthesized AuNPs were employed for a rapid and straightforward AuNPs-based sensing probe for the detection of mercury ions (Hg²⁺) in aqueous samples and merbromin medicine, exhibiting fluorescence at 793 nm. The sensing probe was established using a fluorescence quenching approach for selective detection of Hg²⁺. Results indicate that the AuNPs demonstrate high selectivity for Hg²⁺ compared to other cations, including Pb²⁺, Cd²⁺, Fe²⁺, Ni²⁺, Ba²⁺, Zn²⁺, Cu²⁺, Ag⁺ and Cr⁶⁺. A strong correlation between Hg²⁺ concentration and the observed fluorescence intensity ratio (F₀/F) enables precise quantitative detection, with a limit of detection (LOD) of 0.60 µg/mL in water samples. This study highlights the effectiveness of the fluorescence quenching method as a cost-effective, rapid, and simple tool for Hg²⁺ detection in both environmental water samples and medicinal applications.

Original language	English
Article number	103327
Pages (from-to)	659-668
Number of pages	10
Journal	Photochemical and Photobiological Sciences
Volume	24
Issue number	4
DOIs	https://doi.org/10.1007/s43630-025-00718-7
State	Published - Apr 2025

Funding

The authors express gratitude to DBT and DST, New Delhi, for the support under Star College Scheme (HRD-11011/11/2022-HRD-DBT) and DST-FIST program (No. SR/FST/college-151/2013 (C)) to Jaysingpur College. Author SS acknowledges support from the National Research Foundation, South Korea (Brain Pool Grant No. RS-2024-00445146). Dr. Xiao-Ying Yu received support from the Laboratory Directed Research and Development program at Oak Ridge National Laboratory (ORNL). This manuscript, authored by UT-Battelle, LLC, was prepared under Contract No. DE-AC05-00OR22725 with the U.S. DOE, with government rights for public access as outlined in the DOE Public Access Plan

Keywords

Acacia concinna
AuNPs
Bio-mediated
Hg sensing
Sensing probe

Access to Document

10.1007/s43630-025-00718-7

Cite this

@article{37f546a607b64772a00122aa22ffbecf,

title = "Bio-stabilized AuNPs as a reliable fluorescence sensing probe for Hg2+: application for environmental water sample",

abstract = "Gold nanoparticles (AuNPs) have gained attention due to their unique optical and electronic properties. Their exceptional stability, high conductivity, and strong light interaction also make them ideal for sensing applications. In this study, we developed a green microwave-assisted approach for synthesizing uniform (\textasciitilde{} 10 nm) AuNPs using Acacia concinna fruit extract as a bio-stabilizing agent. Synthesized AuNPs were employed for a rapid and straightforward AuNPs-based sensing probe for the detection of mercury ions (Hg2+) in aqueous samples and merbromin medicine, exhibiting fluorescence at 793 nm. The sensing probe was established using a fluorescence quenching approach for selective detection of Hg2⁺. Results indicate that the AuNPs demonstrate high selectivity for Hg2⁺ compared to other cations, including Pb2⁺, Cd2⁺, Fe2⁺, Ni2⁺, Ba2⁺, Zn2⁺, Cu2+, Ag+ and Cr6+. A strong correlation between Hg2⁺ concentration and the observed fluorescence intensity ratio (F₀/F) enables precise quantitative detection, with a limit of detection (LOD) of 0.60 µg/mL in water samples. This study highlights the effectiveness of the fluorescence quenching method as a cost-effective, rapid, and simple tool for Hg2⁺ detection in both environmental water samples and medicinal applications.",

keywords = "Acacia concinna, AuNPs, Bio-mediated, Hg sensing, Sensing probe",

author = "Shubhangi Mane-Gavade and Patil, \{Supriya A.\} and Vidhya Jadhav and Akshata Pattanshetti and Sandip Nipane and Yu, \{Xiao Ying\} and Kim, \{Deok Kee\} and Sandip Sabale",

note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive licence to the European Photochemistry Association, European Society for Photobiology 2025.",

year = "2025",

month = apr,

doi = "10.1007/s43630-025-00718-7",

language = "English",

volume = "24",

pages = "659--668",

journal = "Photochemical and Photobiological Sciences",

issn = "1474-905X",

publisher = "Springer",

number = "4",

}

TY - JOUR

T1 - Bio-stabilized AuNPs as a reliable fluorescence sensing probe for Hg2+

T2 - application for environmental water sample

AU - Mane-Gavade, Shubhangi

AU - Patil, Supriya A.

AU - Jadhav, Vidhya

AU - Pattanshetti, Akshata

AU - Nipane, Sandip

AU - Yu, Xiao Ying

AU - Kim, Deok Kee

AU - Sabale, Sandip

N1 - Publisher Copyright: © The Author(s), under exclusive licence to the European Photochemistry Association, European Society for Photobiology 2025.

PY - 2025/4

Y1 - 2025/4

N2 - Gold nanoparticles (AuNPs) have gained attention due to their unique optical and electronic properties. Their exceptional stability, high conductivity, and strong light interaction also make them ideal for sensing applications. In this study, we developed a green microwave-assisted approach for synthesizing uniform (~ 10 nm) AuNPs using Acacia concinna fruit extract as a bio-stabilizing agent. Synthesized AuNPs were employed for a rapid and straightforward AuNPs-based sensing probe for the detection of mercury ions (Hg2+) in aqueous samples and merbromin medicine, exhibiting fluorescence at 793 nm. The sensing probe was established using a fluorescence quenching approach for selective detection of Hg2⁺. Results indicate that the AuNPs demonstrate high selectivity for Hg2⁺ compared to other cations, including Pb2⁺, Cd2⁺, Fe2⁺, Ni2⁺, Ba2⁺, Zn2⁺, Cu2+, Ag+ and Cr6+. A strong correlation between Hg2⁺ concentration and the observed fluorescence intensity ratio (F₀/F) enables precise quantitative detection, with a limit of detection (LOD) of 0.60 µg/mL in water samples. This study highlights the effectiveness of the fluorescence quenching method as a cost-effective, rapid, and simple tool for Hg2⁺ detection in both environmental water samples and medicinal applications.

AB - Gold nanoparticles (AuNPs) have gained attention due to their unique optical and electronic properties. Their exceptional stability, high conductivity, and strong light interaction also make them ideal for sensing applications. In this study, we developed a green microwave-assisted approach for synthesizing uniform (~ 10 nm) AuNPs using Acacia concinna fruit extract as a bio-stabilizing agent. Synthesized AuNPs were employed for a rapid and straightforward AuNPs-based sensing probe for the detection of mercury ions (Hg2+) in aqueous samples and merbromin medicine, exhibiting fluorescence at 793 nm. The sensing probe was established using a fluorescence quenching approach for selective detection of Hg2⁺. Results indicate that the AuNPs demonstrate high selectivity for Hg2⁺ compared to other cations, including Pb2⁺, Cd2⁺, Fe2⁺, Ni2⁺, Ba2⁺, Zn2⁺, Cu2+, Ag+ and Cr6+. A strong correlation between Hg2⁺ concentration and the observed fluorescence intensity ratio (F₀/F) enables precise quantitative detection, with a limit of detection (LOD) of 0.60 µg/mL in water samples. This study highlights the effectiveness of the fluorescence quenching method as a cost-effective, rapid, and simple tool for Hg2⁺ detection in both environmental water samples and medicinal applications.

KW - Acacia concinna

KW - AuNPs

KW - Bio-mediated

KW - Hg sensing

KW - Sensing probe

UR - http://www.scopus.com/inward/record.url?scp=105003780079&partnerID=8YFLogxK

U2 - 10.1007/s43630-025-00718-7

DO - 10.1007/s43630-025-00718-7

M3 - Article

AN - SCOPUS:105003780079

SN - 1474-905X

VL - 24

SP - 659

EP - 668

JO - Photochemical and Photobiological Sciences

JF - Photochemical and Photobiological Sciences

IS - 4

M1 - 103327

ER -