Abstract
Digital biosensor systems analyzing biomarkers characteristic of liver injury (LI), soft tissue injury (STI) and abdominal trauma (ABT) were developed and optimized for their performance in serum solutions spiked with injury biomarkers in order to mimic real medical samples. The systems produced 'Alert'-type optical output signals in the form of "YES-NO" separated by a threshold value. The new approach aims at the reliable detection of injury biomarkers for making autonomous decisions towards timely therapeutic interventions, particularly in conditions when a hospital treatment is not possible. The enzyme-catalyzed reactions performing Boolean AND/NAND logic operations in the presence of different combinations of the injury biomarkers allowed high-fidelity biosensing. Robustness of the systems was confirmed by their operation in serum solutions, representing the first example of chemically performed logic analysis of biological fluids and a step closer towards practical biomedical applications of enzyme-logic bioassays.
| Original language | English |
|---|---|
| Pages (from-to) | 955-959 |
| Number of pages | 5 |
| Journal | Talanta |
| Volume | 83 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 15 2011 |
| Externally published | Yes |
Funding
This work was supported by the Office of Naval Research in the frame of the project: “Integrated Enzyme-Logic Systems for “Sense and Treat” Injured Soldiers” (Award #N00014-08-1-1202).
| Funders | Funder number |
|---|---|
| Office of Naval Research | 00014-08-1-1202 |
Keywords
- Abdominal trauma
- Biosensor
- Liver injury
- Logic gate
- Serum
- Soft tissue injury