Bilayer thickness and thermal response of dimyristoylphosphatidylcholine unilamellar vesicles containing cholesterol, ergosterol and lanosterol: A small-angle neutron scattering study

Jeremy Pencer; Mu Ping Nieh; Thad A. Harroun; Susan Krueger; Carl Adams; John Katsaras

doi:10.1016/j.bbamem.2005.10.017

Bilayer thickness and thermal response of dimyristoylphosphatidylcholine unilamellar vesicles containing cholesterol, ergosterol and lanosterol: A small-angle neutron scattering study

Jeremy Pencer, Mu Ping Nieh, Thad A. Harroun, Susan Krueger, Carl Adams, John Katsaras

Research output: Contribution to journal › Article › peer-review

98 Scopus citations

Abstract

Small-angle neutron scattering (SANS) measurements are performed on pure dimyristoyl phosphatidylcholine (DMPC) unilamellar vesicles (ULV) and those containing either 20 or 47 mol% cholesterol, ergosterol or lanosterol. From the SANS data, we were able to determine the influence of these sterols on ULV bilayer thickness and vesicle area expansion coefficients. While these parameters have been determined previously for membranes containing cholesterol, to the best of our knowledge, this is the first time such results have been presented for membranes containing the structurally related sterols, ergosterol and lanosterol. At both molar concentrations and at temperatures ranging from 10 to 45°C, the addition of the different sterols leads to increases in bilayer thickness, relative to pure DMPC. We observe large differences in the influence of these sterols on the membrane thermal area expansion coefficient. All three sterols, however, produce very similar changes to membrane thickness.

Original language	English
Pages (from-to)	84-91
Number of pages	8
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1720
Issue number	1-2
DOIs	https://doi.org/10.1016/j.bbamem.2005.10.017
State	Published - Dec 30 2005
Externally published	Yes

Funding

The authors thank Amy C. Rowat for valuable discussions and criticism of earlier versions of this work and the anonymous reviewers for their helpful comments and suggestions. We acknowledge the support of the National Institute of Standards and Technology, U.S. Department of Commerce in providing the neutron research facilities used in this work and The Advanced Foods and Materials Network (Networks of Centres of Excellence, Canada) for financial assistance. This study was also performed in part with support from the National Institutes of Health grant no. 1 R01 RR14812 and the Regents of the University of California through contributions from the Cold Neutrons for Biology and Technology (CNBT) research partnership.

Funders	Funder number
Cold Neutrons for Biology and Technology
National Institutes of Health
National Center for Research Resources	R01RR014812
National Institute of Standards and Technology
U.S. Department of Commerce
Advanced Foods and Materials Network

Keywords

Cholesterol
Dimyrisotylphosphatidylcholine (DMPC)
Ergosterol
Hydrophobic thickness
Lanosterol
Small-angle neutron scattering
Unilamellar vesicle

Access to Document

10.1016/j.bbamem.2005.10.017

Cite this

Pencer, J., Nieh, M. P., Harroun, T. A., Krueger, S., Adams, C., & Katsaras, J. (2005). Bilayer thickness and thermal response of dimyristoylphosphatidylcholine unilamellar vesicles containing cholesterol, ergosterol and lanosterol: A small-angle neutron scattering study. Biochimica et Biophysica Acta - Biomembranes, 1720(1-2), 84-91. https://doi.org/10.1016/j.bbamem.2005.10.017

@article{a1a133c3674f4ab1940fc2fc7eb7270e,

title = "Bilayer thickness and thermal response of dimyristoylphosphatidylcholine unilamellar vesicles containing cholesterol, ergosterol and lanosterol: A small-angle neutron scattering study",

abstract = "Small-angle neutron scattering (SANS) measurements are performed on pure dimyristoyl phosphatidylcholine (DMPC) unilamellar vesicles (ULV) and those containing either 20 or 47 mol% cholesterol, ergosterol or lanosterol. From the SANS data, we were able to determine the influence of these sterols on ULV bilayer thickness and vesicle area expansion coefficients. While these parameters have been determined previously for membranes containing cholesterol, to the best of our knowledge, this is the first time such results have been presented for membranes containing the structurally related sterols, ergosterol and lanosterol. At both molar concentrations and at temperatures ranging from 10 to 45°C, the addition of the different sterols leads to increases in bilayer thickness, relative to pure DMPC. We observe large differences in the influence of these sterols on the membrane thermal area expansion coefficient. All three sterols, however, produce very similar changes to membrane thickness.",

keywords = "Cholesterol, Dimyrisotylphosphatidylcholine (DMPC), Ergosterol, Hydrophobic thickness, Lanosterol, Small-angle neutron scattering, Unilamellar vesicle",

author = "Jeremy Pencer and Nieh, {Mu Ping} and Harroun, {Thad A.} and Susan Krueger and Carl Adams and John Katsaras",

year = "2005",

month = dec,

day = "30",

doi = "10.1016/j.bbamem.2005.10.017",

language = "English",

volume = "1720",

pages = "84--91",

journal = "Biochimica et Biophysica Acta - Biomembranes",

issn = "0005-2736",

publisher = "Elsevier",

number = "1-2",

}

Pencer, J, Nieh, MP, Harroun, TA, Krueger, S, Adams, C & Katsaras, J 2005, 'Bilayer thickness and thermal response of dimyristoylphosphatidylcholine unilamellar vesicles containing cholesterol, ergosterol and lanosterol: A small-angle neutron scattering study', Biochimica et Biophysica Acta - Biomembranes, vol. 1720, no. 1-2, pp. 84-91. https://doi.org/10.1016/j.bbamem.2005.10.017

Bilayer thickness and thermal response of dimyristoylphosphatidylcholine unilamellar vesicles containing cholesterol, ergosterol and lanosterol: A small-angle neutron scattering study. / Pencer, Jeremy; Nieh, Mu Ping; Harroun, Thad A. et al.
In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1720, No. 1-2, 30.12.2005, p. 84-91.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Bilayer thickness and thermal response of dimyristoylphosphatidylcholine unilamellar vesicles containing cholesterol, ergosterol and lanosterol

T2 - A small-angle neutron scattering study

AU - Pencer, Jeremy

AU - Nieh, Mu Ping

AU - Harroun, Thad A.

AU - Krueger, Susan

AU - Adams, Carl

AU - Katsaras, John

PY - 2005/12/30

Y1 - 2005/12/30

N2 - Small-angle neutron scattering (SANS) measurements are performed on pure dimyristoyl phosphatidylcholine (DMPC) unilamellar vesicles (ULV) and those containing either 20 or 47 mol% cholesterol, ergosterol or lanosterol. From the SANS data, we were able to determine the influence of these sterols on ULV bilayer thickness and vesicle area expansion coefficients. While these parameters have been determined previously for membranes containing cholesterol, to the best of our knowledge, this is the first time such results have been presented for membranes containing the structurally related sterols, ergosterol and lanosterol. At both molar concentrations and at temperatures ranging from 10 to 45°C, the addition of the different sterols leads to increases in bilayer thickness, relative to pure DMPC. We observe large differences in the influence of these sterols on the membrane thermal area expansion coefficient. All three sterols, however, produce very similar changes to membrane thickness.

AB - Small-angle neutron scattering (SANS) measurements are performed on pure dimyristoyl phosphatidylcholine (DMPC) unilamellar vesicles (ULV) and those containing either 20 or 47 mol% cholesterol, ergosterol or lanosterol. From the SANS data, we were able to determine the influence of these sterols on ULV bilayer thickness and vesicle area expansion coefficients. While these parameters have been determined previously for membranes containing cholesterol, to the best of our knowledge, this is the first time such results have been presented for membranes containing the structurally related sterols, ergosterol and lanosterol. At both molar concentrations and at temperatures ranging from 10 to 45°C, the addition of the different sterols leads to increases in bilayer thickness, relative to pure DMPC. We observe large differences in the influence of these sterols on the membrane thermal area expansion coefficient. All three sterols, however, produce very similar changes to membrane thickness.

KW - Cholesterol

KW - Dimyrisotylphosphatidylcholine (DMPC)

KW - Ergosterol

KW - Hydrophobic thickness

KW - Lanosterol

KW - Small-angle neutron scattering

KW - Unilamellar vesicle

UR - http://www.scopus.com/inward/record.url?scp=32044462710&partnerID=8YFLogxK

U2 - 10.1016/j.bbamem.2005.10.017

DO - 10.1016/j.bbamem.2005.10.017

M3 - Article

C2 - 16386704

AN - SCOPUS:32044462710

SN - 0005-2736

VL - 1720

SP - 84

EP - 91

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

IS - 1-2

ER -

Bilayer thickness and thermal response of dimyristoylphosphatidylcholine unilamellar vesicles containing cholesterol, ergosterol and lanosterol: A small-angle neutron scattering study

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this