Bacterial growth phase influences methylmercury production by the sulfate-reducing bacterium desulfovibrio desulfuricans nd132

Abir Biswas; Scott C. Brooks; Carrie L. Miller; Jennifer J. Mosher; Xiangping L. Yin; Meghan M. Drake

doi:10.1016/j.scitotenv.2011.06.037

Bacterial growth phase influences methylmercury production by the sulfate-reducing bacterium desulfovibrio desulfuricans nd132

Abir Biswas, Scott C. Brooks, Carrie L. Miller, Jennifer J. Mosher, Xiangping L. Yin, Meghan M. Drake

Biogeochemical Dynamics

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Abstract

The effect of bacterial growth phase is an aspect of mercury (Hg) methylation that previous studies have not investigated in detail. Here we consider the effect of growth phase (mid-log, late-log and late stationary phase) on Hg methylation by the known methylator Desulfovibrio desulfuricans ND132. We tested the addition of Hg alone (chloride-complex), Hg with Suwannee River natural organic matter (SRNOM) (unequilibrated), and Hg equilibrated with SRNOM on monomethylmercury (MMHg) production by ND132 over a growth curve in pyruvate-fumarate media. This NOM did not affect MMHg production even under very low Hg:SRNOM ratios, where Hg binding is predicted to be dominated by high energy sites. Adding Hg or Hg-NOM to growing cultures 24. h before sampling (late addition) resulted in ~. 2× greater net fraction of Hg methylated than for comparably aged cultures exposed to Hg from the initial culture inoculation (early addition). Mid- and late-log phase cultures produced similar amounts of MMHg, but late stationary phase cultures (both under early and late Hg addition conditions) produced up to ~. 3× more MMHg, indicating the potential importance of growth phase in studies of MMHg production.

Original language	English
Pages (from-to)	3943-3948
Number of pages	6
Journal	Science of the Total Environment
Volume	409
Issue number	19
DOIs	https://doi.org/10.1016/j.scitotenv.2011.06.037
State	Published - Sep 1 2011

Funding

The authors thank Dr. J. Moberly and G. Southworth for helpful discussions and aid with analyses and C. Brandt for aid with statistical analyses. They also thank Dr. C. Gilmour for cultures of ND132 and Dr. D. Elias and Dr. J. Wall and for media protocols. We also appreciate the thoughtful review comments that improved the manuscript. We provide details of experimental conditions and methods as well as NOM characterization in Supporting material. This work was funded by the U.S. Department of Energy , Office of Science, Biological and Environmental Research, Subsurface Biogeochemical Research Program and is a product of the Science Focus Area (SFA) at ORNL. ORNL is managed by UT-Battelle LLC for the U.S. Department of Energy under contract DEAC05-00OR22725 .

Funders	Funder number
UT-Battelle LLC	DEAC05-00OR22725
U.S. Department of Energy
Office of Science
Biological and Environmental Research
Oak Ridge National Laboratory

Keywords

Desulfovibrio desulfuricans ND132
Growth stage
Mercury
Methylation
Monomethylmercury

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10.1016/j.scitotenv.2011.06.037

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title = "Bacterial growth phase influences methylmercury production by the sulfate-reducing bacterium desulfovibrio desulfuricans nd132",

abstract = "The effect of bacterial growth phase is an aspect of mercury (Hg) methylation that previous studies have not investigated in detail. Here we consider the effect of growth phase (mid-log, late-log and late stationary phase) on Hg methylation by the known methylator Desulfovibrio desulfuricans ND132. We tested the addition of Hg alone (chloride-complex), Hg with Suwannee River natural organic matter (SRNOM) (unequilibrated), and Hg equilibrated with SRNOM on monomethylmercury (MMHg) production by ND132 over a growth curve in pyruvate-fumarate media. This NOM did not affect MMHg production even under very low Hg:SRNOM ratios, where Hg binding is predicted to be dominated by high energy sites. Adding Hg or Hg-NOM to growing cultures 24. h before sampling (late addition) resulted in ~. 2× greater net fraction of Hg methylated than for comparably aged cultures exposed to Hg from the initial culture inoculation (early addition). Mid- and late-log phase cultures produced similar amounts of MMHg, but late stationary phase cultures (both under early and late Hg addition conditions) produced up to ~. 3× more MMHg, indicating the potential importance of growth phase in studies of MMHg production.",

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AB - The effect of bacterial growth phase is an aspect of mercury (Hg) methylation that previous studies have not investigated in detail. Here we consider the effect of growth phase (mid-log, late-log and late stationary phase) on Hg methylation by the known methylator Desulfovibrio desulfuricans ND132. We tested the addition of Hg alone (chloride-complex), Hg with Suwannee River natural organic matter (SRNOM) (unequilibrated), and Hg equilibrated with SRNOM on monomethylmercury (MMHg) production by ND132 over a growth curve in pyruvate-fumarate media. This NOM did not affect MMHg production even under very low Hg:SRNOM ratios, where Hg binding is predicted to be dominated by high energy sites. Adding Hg or Hg-NOM to growing cultures 24. h before sampling (late addition) resulted in ~. 2× greater net fraction of Hg methylated than for comparably aged cultures exposed to Hg from the initial culture inoculation (early addition). Mid- and late-log phase cultures produced similar amounts of MMHg, but late stationary phase cultures (both under early and late Hg addition conditions) produced up to ~. 3× more MMHg, indicating the potential importance of growth phase in studies of MMHg production.

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Bacterial growth phase influences methylmercury production by the sulfate-reducing bacterium desulfovibrio desulfuricans nd132

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