Atractiella rhizophila, sp. Nov., an endorrhizal fungus isolated from the populus root microbiome

Gregory Bonito; Khalid Hameed; Merje Toome-Heller; Rosanne Healy; Chantal Reid; Hui Ling Liao; M. Catherine Aime; Christopher Schadt; Rytas Vilgalys

doi:10.1080/00275514.2016.1271689

Atractiella rhizophila, sp. Nov., an endorrhizal fungus isolated from the populus root microbiome

Gregory Bonito, Khalid Hameed, Merje Toome-Heller, Rosanne Healy, Chantal Reid, Hui Ling Liao, M. Catherine Aime, Christopher Schadt, Rytas Vilgalys

Integrative Microbiomics

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Among fungi isolated from healthy root mycobiomes of Populus, we discovered a new endorrhizal fungal species belonging to the rust lineage Pucciniomycotina, described here as Atractiella rhizophila. We characterized this species by transmission electron microscopy (TEM), phylogenetic analysis, and plant bioassay experiments. Phylogenetic sequence analysis of isolates and available environmental and reference sequences indicates that this new species, A. rhizophila, has a broad geographic and host range. Atractiella rhizophila appears to be present in North America, Australia, Asia, and Africa and is associated with trees, orchids, and other agriculturally important species, including soybean, corn, and rice. Despite the large geographic and host range of this species sampling, A. rhizophila appears to have exceptionally low sequence variation within nuclear rDNA markers examined. With inoculation studies, we demonstrate that A. rhizophila is nonpathogenic, asymptomatically colonizes plant roots, and appears to foster plant growth and elevated photosynthesis rates.

Original language	English
Pages (from-to)	18-26
Number of pages	9
Journal	Mycologia
Volume	109
Issue number	1
DOIs	https://doi.org/10.1080/00275514.2016.1271689
State	Published - Jan 9 2017

Funding

This research was sponsored by the Genomic Science Program, US Department of Energy, Office of Science— Biological and Environmental Research, as part of the Plant Microbe Interfaces Scientific Focus Area (http://pmi.ornl.gov). Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the US Department of Energy under contract DE-AC05-00OR22725. G.B. acknowledges AgBioResearch and Michigan State University for support. The authors thank Kevin Le for assistance with the photosynthetic measurements and Connie Robertson of the Duke Herbarium for assistance with fungal curation.

Funders	Funder number
Office of Science— Biological and Environmental Research
Plant Microbe Interfaces Scientific Focus Area
US Department of Energy
Oak Ridge National Laboratory	DE-AC05-00OR22725
Michigan State University

Keywords

Basidiomycete
Plant microbiome
Root endophyte
Sterile mycelium

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/00275514.2016.1271689

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title = "Atractiella rhizophila, sp. Nov., an endorrhizal fungus isolated from the populus root microbiome",

abstract = "Among fungi isolated from healthy root mycobiomes of Populus, we discovered a new endorrhizal fungal species belonging to the rust lineage Pucciniomycotina, described here as Atractiella rhizophila. We characterized this species by transmission electron microscopy (TEM), phylogenetic analysis, and plant bioassay experiments. Phylogenetic sequence analysis of isolates and available environmental and reference sequences indicates that this new species, A. rhizophila, has a broad geographic and host range. Atractiella rhizophila appears to be present in North America, Australia, Asia, and Africa and is associated with trees, orchids, and other agriculturally important species, including soybean, corn, and rice. Despite the large geographic and host range of this species sampling, A. rhizophila appears to have exceptionally low sequence variation within nuclear rDNA markers examined. With inoculation studies, we demonstrate that A. rhizophila is nonpathogenic, asymptomatically colonizes plant roots, and appears to foster plant growth and elevated photosynthesis rates.",

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AU - Hameed, Khalid

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AU - Reid, Chantal

AU - Liao, Hui Ling

AU - Aime, M. Catherine

AU - Schadt, Christopher

AU - Vilgalys, Rytas

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KW - Sterile mycelium

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Atractiella rhizophila, sp. Nov., an endorrhizal fungus isolated from the populus root microbiome

Abstract

Funding

Keywords

UN SDGs

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