Atomic Force Microscopy of Single- and Double-Stranded Deoxyribonucleic Acid

T. Thundat; D. P. Allison; R. J. Warmack

doi:10.1116/1.578312

Atomic Force Microscopy of Single- and Double-Stranded Deoxyribonucleic Acid

T. Thundat, D. P. Allison, R. J. Warmack

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44 Scopus citations

Abstract

The atomic force microscope (AFM) was used to image double-stranded DNA and single-stranded DNA adsorbed onto mica modified by Ba (II). Images of double-stranded DNA show an open circular shape without drastic contortions, and contour lengths that measure within 1% of the calculated length. Images of single-stranded DNA show two distinct forms; compact open circles with “nodes or lumps almost uniformly distributed are the most frequently observed while less abundant are highly elongated circles with a few nodes, Heating the single-stranded DNA sample and altering the solvent conditions prior to adsorption was found to increase the number of elongated circular molecules observed in the AFM images.

Original language	English
Pages (from-to)	824-828
Number of pages	5
Journal	Journal of Vacuum Science and Technology, Part A: Vacuum, Surfaces and Films
Volume	11
Issue number	4
DOIs	https://doi.org/10.1116/1.578312
State	Published - Jul 1993
Externally published	Yes

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title = "Atomic Force Microscopy of Single- and Double-Stranded Deoxyribonucleic Acid",

abstract = "The atomic force microscope (AFM) was used to image double-stranded DNA and single-stranded DNA adsorbed onto mica modified by Ba (II). Images of double-stranded DNA show an open circular shape without drastic contortions, and contour lengths that measure within 1% of the calculated length. Images of single-stranded DNA show two distinct forms; compact open circles with “nodes or lumps almost uniformly distributed are the most frequently observed while less abundant are highly elongated circles with a few nodes, Heating the single-stranded DNA sample and altering the solvent conditions prior to adsorption was found to increase the number of elongated circular molecules observed in the AFM images.",

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Atomic Force Microscopy of Single- and Double-Stranded Deoxyribonucleic Acid

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