Abstract
Invasive microbes causing diseases such as sudden oak death negatively affect ecosystems and economies around the world. The deployment of resistant genotypes for combating introduced diseases typically relies on breeding programs that can take decades to complete. To demonstrate how this process can be accelerated, we employed a genome-wide association mapping of ca. 1,000 resequenced Populus trichocarpa trees individually challenged with Sphaerulina musiva, an invasive fungal pathogen. Among significant associations, three loci associated with resistancewere identified and predicted to encode one putative membrane-bound L-type receptorlike kinase and two receptor-like proteins. A susceptibility-associated locus was predicted to encode a putative G-type D-mannose-binding receptor-like kinase. Multiple lines of evidence, including allele analysis, transcriptomics, binding assays, and overexpression, support the hypothesized function of these candidate genes in the P. trichocarpa response to S. musiva.
Original language | English |
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Pages (from-to) | 11573-11578 |
Number of pages | 6 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 115 |
Issue number | 45 |
DOIs | |
State | Published - Nov 6 2018 |
Funding
ACKNOWLEDGMENTS. This study was supported by Department of Energy (DOE) Office of Science, Office of Biological and Environmental Research (BER) Grant DE-SC0018196, US Department of Agriculture Grant 2012-34103-19771 (to J.M.L.), the Plant–Microbe Interfaces Scientific Focus Area in the DOE BER Genomic Science Program, and the DOE Center for Bioenergy Innovation Project. The Center for Bioenergy Innovation is a US DOE Bioenergy Research Center supported by the Office of BER in the DOE Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle, LLC for the US DOE under Contract DE-AC05-00OR22725. The work conducted by the US DOE Joint Genome Institute is supported by the Office of Science of the US DOE under Contract DE-AC02-05CH11231. This work was also supported by National Institute of Food and Agriculture, US Department of Agriculture Award 2014-51181-22384 (to J.H.C.). J.-Y.Y. and K.W.M. were supported by NIH Grants P41GM103390 and P01GM107012. We would also like to acknowledge the DOE funded Center for Plant and Microbial Complex Carbohydrates Grant DE-SC0015662 (DE-FG02-93ER20097) for equipment support. This research used resources of the Compute and Data Environment for Science (CADES) at the Oak Ridge National Laboratory.
Funders | Funder number |
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DOE Center for Bioenergy Innovation Project | |
DOE Office of Science | |
DOE funded Center for Plant and Microbial Complex Carbohydrates | DE-FG02-93ER20097, DE-SC0015662 |
Office of BER | |
Office of Biological and Environmental Research | |
DOE Office of Science | DE-AC02-05CH11231 |
US Department of Agriculture | 2012-34103-19771 |
National Institutes of Health | P41GM103390 |
U.S. Department of Energy | |
National Institute of General Medical Sciences | P01GM107012 |
National Institute of Food and Agriculture | 2014-51181-22384 |
Office of Science | |
Biological and Environmental Research | DE-SC0018196 |
Oak Ridge National Laboratory | DE-AC05-00OR22725 |
Keywords
- Association mapping
- Disease resistance
- Invasive disease
- Populus trichocarpa
- Septoria canker