Assessing the pulmonary toxicity of single-walled carbon nanohorns

Rachel M. Lynch; Brynn H. Voy; Dana F. Glass; Shannon M. Mahurin; Bin Zhao; Hui Hu; Arnold M. Saxton; Robert L. Donnell; Meng Dawn Cheng

doi:10.1080/17435390701598496

Assessing the pulmonary toxicity of single-walled carbon nanohorns

Rachel M. Lynch, Brynn H. Voy, Dana F. Glass, Shannon M. Mahurin, Bin Zhao, Hui Hu, Arnold M. Saxton, Robert L. Donnell, Meng Dawn Cheng

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Previous studies have suggested that single-walled carbon nanotubes (SWCNTs) may pose a pulmonary hazard. We investigated the pulmonary toxicity of single-walled carbon nanohorns (SWCNHs), a relatively new carbon-based nanomaterial that is structurally similar to SWCNTs. Mice were exposed to 30 μg of surfactant-suspended SWCNHs or an equal volume of vehicle control by pharyngeal aspiration and sacrificed 24 hours or 7 days post-exposure. Total and differential cell counts and cytokine analysis of bronchoalveolar lavage fluid demonstrated a mild inflammatory response which was mitigated by day 7 post-exposure. Whole lung microarray analysis demonstrated that SWCNH-exposure did not lead to robust changes in gene expression. Finally, histological analysis showed no evidence of granuloma formation or fibrosis following SWCNH aspiration. These combined results suggest that SWCNH is a relatively innocuous nanomaterial when delivered to mice in vivo using aspiration as a delivery mechanism.

Original language	English
Pages (from-to)	157-166
Number of pages	10
Journal	Nanotoxicology
Volume	1
Issue number	2
DOIs	https://doi.org/10.1080/17435390701598496
State	Published - Jun 2007

Funding

This research was sponsored by the Laboratory Directed Research and Development Program of Oak Ridge National Laboratory (ORNL), managed by UT-Battelle, LLC, for the US Department of Energy under Contract No. DE-AC05-00OR22725. We are indebted to Dr Vincent Castranova of the US National Institute of Occupational Safety and Health for training us in the aspiration and BAL techniques used in this study. We thank Dr David Geohegan of the Materials Science and Technology Division at ORNL for valuable discussion on laser synthesis of SWCNHs during the study. We also thank Dr John Dunlap of the University of Tennessee Knoxville (UTK) for his assistance with SEM imaging and Dr Micheal Karlstad of the Division of Surgical Research at the UTK Graduate School of Medicine for advice and use of his Laboratory.

Funders	Funder number
US Department of Energy
UT-Battelle
Oak Ridge National Laboratory

Keywords

Aspiration
Inflammatory
Nanotoxicology
Single-walled carbon nanohorns

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title = "Assessing the pulmonary toxicity of single-walled carbon nanohorns",

abstract = "Previous studies have suggested that single-walled carbon nanotubes (SWCNTs) may pose a pulmonary hazard. We investigated the pulmonary toxicity of single-walled carbon nanohorns (SWCNHs), a relatively new carbon-based nanomaterial that is structurally similar to SWCNTs. Mice were exposed to 30 μg of surfactant-suspended SWCNHs or an equal volume of vehicle control by pharyngeal aspiration and sacrificed 24 hours or 7 days post-exposure. Total and differential cell counts and cytokine analysis of bronchoalveolar lavage fluid demonstrated a mild inflammatory response which was mitigated by day 7 post-exposure. Whole lung microarray analysis demonstrated that SWCNH-exposure did not lead to robust changes in gene expression. Finally, histological analysis showed no evidence of granuloma formation or fibrosis following SWCNH aspiration. These combined results suggest that SWCNH is a relatively innocuous nanomaterial when delivered to mice in vivo using aspiration as a delivery mechanism.",

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author = "Lynch, {Rachel M.} and Voy, {Brynn H.} and Glass, {Dana F.} and Mahurin, {Shannon M.} and Bin Zhao and Hui Hu and Saxton, {Arnold M.} and Donnell, {Robert L.} and Cheng, {Meng Dawn}",

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AU - Lynch, Rachel M.

AU - Voy, Brynn H.

AU - Glass, Dana F.

AU - Mahurin, Shannon M.

AU - Zhao, Bin

AU - Hu, Hui

AU - Saxton, Arnold M.

AU - Donnell, Robert L.

AU - Cheng, Meng Dawn

PY - 2007/6

Y1 - 2007/6

N2 - Previous studies have suggested that single-walled carbon nanotubes (SWCNTs) may pose a pulmonary hazard. We investigated the pulmonary toxicity of single-walled carbon nanohorns (SWCNHs), a relatively new carbon-based nanomaterial that is structurally similar to SWCNTs. Mice were exposed to 30 μg of surfactant-suspended SWCNHs or an equal volume of vehicle control by pharyngeal aspiration and sacrificed 24 hours or 7 days post-exposure. Total and differential cell counts and cytokine analysis of bronchoalveolar lavage fluid demonstrated a mild inflammatory response which was mitigated by day 7 post-exposure. Whole lung microarray analysis demonstrated that SWCNH-exposure did not lead to robust changes in gene expression. Finally, histological analysis showed no evidence of granuloma formation or fibrosis following SWCNH aspiration. These combined results suggest that SWCNH is a relatively innocuous nanomaterial when delivered to mice in vivo using aspiration as a delivery mechanism.

AB - Previous studies have suggested that single-walled carbon nanotubes (SWCNTs) may pose a pulmonary hazard. We investigated the pulmonary toxicity of single-walled carbon nanohorns (SWCNHs), a relatively new carbon-based nanomaterial that is structurally similar to SWCNTs. Mice were exposed to 30 μg of surfactant-suspended SWCNHs or an equal volume of vehicle control by pharyngeal aspiration and sacrificed 24 hours or 7 days post-exposure. Total and differential cell counts and cytokine analysis of bronchoalveolar lavage fluid demonstrated a mild inflammatory response which was mitigated by day 7 post-exposure. Whole lung microarray analysis demonstrated that SWCNH-exposure did not lead to robust changes in gene expression. Finally, histological analysis showed no evidence of granuloma formation or fibrosis following SWCNH aspiration. These combined results suggest that SWCNH is a relatively innocuous nanomaterial when delivered to mice in vivo using aspiration as a delivery mechanism.

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Assessing the pulmonary toxicity of single-walled carbon nanohorns

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