Aryl diazonium chemistry for the surface functionalization of glassy biosensors

Wei Zheng, Remko van den Hurk, Yong Cao, Rongbing Du, Xuejun Sun, Yiyu Wang, Mark T. McDermott, Stephane Evoy

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Nanostring resonator and fiber-optics-based biosensors are of interest as they offer high sensitivity, real-time measurements and the ability to integrate with electronics. However, these devices are somewhat impaired by issues related to surface modification. Both nanostring resonators and photonic sensors employ glassy materials, which are incompatible with electrochemistry. A surface chemistry approach providing strong and stable adhesion to glassy surfaces is thus required. In this work, a diazonium salt induced aryl film grafting process is employed to modify a novel SiCN glassy material. Sandwich rabbit IgG binding assays are performed on the diazonium treated SiCN surfaces. Fluorescently labelled anti-rabbit IgG and anti-rabbit IgG conjugated gold nanoparticles were used as markers to demonstrate the absorption of anti-rabbit IgG and therefore verify the successful grafting of the aryl film. The results of the experiments support the effectiveness of diazonium chemistry for the surface functionalization of SiCN surfaces. This method is applicable to other types of glassy materials and potentially can be expanded to various nanomechanical and optical biosensors.

Original languageEnglish
Article number8
JournalBiosensors
Volume6
Issue number1
DOIs
StatePublished - Mar 14 2016
Externally publishedYes

Keywords

  • Biosensors
  • Diazonium
  • Glasses
  • Nanostrings
  • Optical fibers
  • Surface linkers

Fingerprint

Dive into the research topics of 'Aryl diazonium chemistry for the surface functionalization of glassy biosensors'. Together they form a unique fingerprint.

Cite this