Arabidopsis NITROGEN LIMITATION ADAPTATION regulates ORE1 homeostasis during senescence induced by nitrogen deficiency

Bong Soo Park; Tao Yao; Jun Sung Seo; Eriko Chi Cheng Wong; Nobutaka Mitsuda; Chung Hao Huang; Nam Hai Chua

doi:10.1038/s41477-018-0269-8

Arabidopsis NITROGEN LIMITATION ADAPTATION regulates ORE1 homeostasis during senescence induced by nitrogen deficiency

Bong Soo Park, Tao Yao, Jun Sung Seo, Eriko Chi Cheng Wong, Nobutaka Mitsuda, Chung Hao Huang, Nam Hai Chua

Research output: Contribution to journal › Letter › peer-review

78 Scopus citations

Abstract

Nitrogen is an important macronutrient in plants and its deficiency induces rapid leaf senescence. Two genes, ORE1 and NITROGEN LIMITATION ADAPTATION (NLA), have been implicated in regulating the senescence process but their relationship is unclear^1,2. Here, we show that nla and pho2 (also known as ubc24) plants develop rapid leaf senescence under nitrogen-starvation condition, whereas ore1 and nla/ore1 and pho2 (ubc24)/ore1 plants stay green. These results suggest that ORE1 acts downstream of NLA and PHO2 (UBC24). NLA interacts with ORE1 in the nucleus and regulates its stability through polyubiquitination using PHO2 (UBC24) as the E2 conjugase. Our findings identified ORE1 as a downstream target of NLA/PHO2 (UBC24) and showed that post-translational regulation of ORE1 levels determines leaf senescence during nitrogen deficiency.

Original language	English
Pages (from-to)	898-903
Number of pages	6
Journal	Nature Plants
Volume	4
Issue number	11
DOIs	https://doi.org/10.1038/s41477-018-0269-8
State	Published - Nov 1 2018
Externally published	Yes

Funding

This work was supported by an RSSS grant (no. NRF-RSSS-002) from the National Research Foundation, Singapore to N.H.C and by a MEXT KAKENHI grant (no. 25113001), Japan to N.M.

Funders	Funder number
National Research Foundation
Ministry of Education, Culture, Sports, Science and Technology	25113001

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title = " Arabidopsis NITROGEN LIMITATION ADAPTATION regulates ORE1 homeostasis during senescence induced by nitrogen deficiency",

abstract = "Nitrogen is an important macronutrient in plants and its deficiency induces rapid leaf senescence. Two genes, ORE1 and NITROGEN LIMITATION ADAPTATION (NLA), have been implicated in regulating the senescence process but their relationship is unclear1,2. Here, we show that nla and pho2 (also known as ubc24) plants develop rapid leaf senescence under nitrogen-starvation condition, whereas ore1 and nla/ore1 and pho2 (ubc24)/ore1 plants stay green. These results suggest that ORE1 acts downstream of NLA and PHO2 (UBC24). NLA interacts with ORE1 in the nucleus and regulates its stability through polyubiquitination using PHO2 (UBC24) as the E2 conjugase. Our findings identified ORE1 as a downstream target of NLA/PHO2 (UBC24) and showed that post-translational regulation of ORE1 levels determines leaf senescence during nitrogen deficiency.",

author = "Park, {Bong Soo} and Tao Yao and Seo, {Jun Sung} and Wong, {Eriko Chi Cheng} and Nobutaka Mitsuda and Huang, {Chung Hao} and Chua, {Nam Hai}",

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AU - Park, Bong Soo

AU - Yao, Tao

AU - Seo, Jun Sung

AU - Wong, Eriko Chi Cheng

AU - Mitsuda, Nobutaka

AU - Huang, Chung Hao

AU - Chua, Nam Hai

PY - 2018/11/1

Y1 - 2018/11/1

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AB - Nitrogen is an important macronutrient in plants and its deficiency induces rapid leaf senescence. Two genes, ORE1 and NITROGEN LIMITATION ADAPTATION (NLA), have been implicated in regulating the senescence process but their relationship is unclear1,2. Here, we show that nla and pho2 (also known as ubc24) plants develop rapid leaf senescence under nitrogen-starvation condition, whereas ore1 and nla/ore1 and pho2 (ubc24)/ore1 plants stay green. These results suggest that ORE1 acts downstream of NLA and PHO2 (UBC24). NLA interacts with ORE1 in the nucleus and regulates its stability through polyubiquitination using PHO2 (UBC24) as the E2 conjugase. Our findings identified ORE1 as a downstream target of NLA/PHO2 (UBC24) and showed that post-translational regulation of ORE1 levels determines leaf senescence during nitrogen deficiency.

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ER -

Arabidopsis NITROGEN LIMITATION ADAPTATION regulates ORE1 homeostasis during senescence induced by nitrogen deficiency

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