Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing

Hannah B. Musgrove; Megan A. Catterton; Rebecca R. Pompano

doi:10.1016/j.aca.2022.339842

Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing

Hannah B. Musgrove
, Megan A. Catterton
, Rebecca R. Pompano

Research output: Contribution to journal › Review article › peer-review

49 Scopus citations

Abstract

Resin 3D printing, especially digital light processing (DLP) printing, is a promising rapid fabrication method for bio-microfluidic applications such as clinical tests, lab-on-a-chip devices, and sensor integrated devices. The benefits of 3D printing lead many to believe this fabrication method will accelerate the use of microfluidics, but there are a number of potential obstacles to overcome for bioanalytical labs to fully utilize this technology. For commercially available printing materials, this includes challenges in producing prints with the print resolution and mechanical stability required for a particular design, along with cytotoxic components within many photopolymerizing resins and low optical compatibility for imaging experiments. Potential solutions to these problems are scattered throughout the literature and rarely available in head-to-head comparisons. Therefore, we present here a concise guide to the principles of resin 3D printing most relevant for fabrication of bioanalytical microfluidic devices. Intended to quickly orient labs that are new to 3D printing, the tutorial includes the results of selected systematic tests to inform resin selection, strategies for design optimization, and improvement of biocompatibility of resin 3D printed bio-microfluidic devices.

Original language	English
Article number	339842
Journal	Analytica Chimica Acta
Volume	1209
DOIs	https://doi.org/10.1016/j.aca.2022.339842
State	Published - May 29 2022
Externally published	Yes

Funding

Research reported in this publication was supported by the National Institute of Allergy and Infectious Diseases under Award No. R01AI131723 and from the National Institute of Biomedical Imaging and Bioengineering under Award No. R03EB028043 through the National Institute of Health (NIH). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The authors would like to thank Amirus Saleheen for his suggestions for post-treating resins in preparation for cell culture, Scott Karas for his assistance with sanding prints, Alexander Ball for his expertise and assistance with flow cytometry experiments, and Michael Ly for his correspondence regarding MiiCraft and CadWorks3D resins and printers.

Keywords

Cell culture
Digital light processing
Microfluidic fabrication
Photopolymerizable resins
SLA
Stereolithography

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10.1016/j.aca.2022.339842

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title = "Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing",

abstract = "Resin 3D printing, especially digital light processing (DLP) printing, is a promising rapid fabrication method for bio-microfluidic applications such as clinical tests, lab-on-a-chip devices, and sensor integrated devices. The benefits of 3D printing lead many to believe this fabrication method will accelerate the use of microfluidics, but there are a number of potential obstacles to overcome for bioanalytical labs to fully utilize this technology. For commercially available printing materials, this includes challenges in producing prints with the print resolution and mechanical stability required for a particular design, along with cytotoxic components within many photopolymerizing resins and low optical compatibility for imaging experiments. Potential solutions to these problems are scattered throughout the literature and rarely available in head-to-head comparisons. Therefore, we present here a concise guide to the principles of resin 3D printing most relevant for fabrication of bioanalytical microfluidic devices. Intended to quickly orient labs that are new to 3D printing, the tutorial includes the results of selected systematic tests to inform resin selection, strategies for design optimization, and improvement of biocompatibility of resin 3D printed bio-microfluidic devices.",

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year = "2022",

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doi = "10.1016/j.aca.2022.339842",

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AU - Musgrove, Hannah B.

AU - Catterton, Megan A.

AU - Pompano, Rebecca R.

PY - 2022/5/29

Y1 - 2022/5/29

N2 - Resin 3D printing, especially digital light processing (DLP) printing, is a promising rapid fabrication method for bio-microfluidic applications such as clinical tests, lab-on-a-chip devices, and sensor integrated devices. The benefits of 3D printing lead many to believe this fabrication method will accelerate the use of microfluidics, but there are a number of potential obstacles to overcome for bioanalytical labs to fully utilize this technology. For commercially available printing materials, this includes challenges in producing prints with the print resolution and mechanical stability required for a particular design, along with cytotoxic components within many photopolymerizing resins and low optical compatibility for imaging experiments. Potential solutions to these problems are scattered throughout the literature and rarely available in head-to-head comparisons. Therefore, we present here a concise guide to the principles of resin 3D printing most relevant for fabrication of bioanalytical microfluidic devices. Intended to quickly orient labs that are new to 3D printing, the tutorial includes the results of selected systematic tests to inform resin selection, strategies for design optimization, and improvement of biocompatibility of resin 3D printed bio-microfluidic devices.

AB - Resin 3D printing, especially digital light processing (DLP) printing, is a promising rapid fabrication method for bio-microfluidic applications such as clinical tests, lab-on-a-chip devices, and sensor integrated devices. The benefits of 3D printing lead many to believe this fabrication method will accelerate the use of microfluidics, but there are a number of potential obstacles to overcome for bioanalytical labs to fully utilize this technology. For commercially available printing materials, this includes challenges in producing prints with the print resolution and mechanical stability required for a particular design, along with cytotoxic components within many photopolymerizing resins and low optical compatibility for imaging experiments. Potential solutions to these problems are scattered throughout the literature and rarely available in head-to-head comparisons. Therefore, we present here a concise guide to the principles of resin 3D printing most relevant for fabrication of bioanalytical microfluidic devices. Intended to quickly orient labs that are new to 3D printing, the tutorial includes the results of selected systematic tests to inform resin selection, strategies for design optimization, and improvement of biocompatibility of resin 3D printed bio-microfluidic devices.

KW - Cell culture

KW - Digital light processing

KW - Microfluidic fabrication

KW - Photopolymerizable resins

KW - SLA

KW - Stereolithography

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DO - 10.1016/j.aca.2022.339842

M3 - Review article

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ER -

Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing

Abstract

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