TY - JOUR
T1 - Applications of mass spectrometry to DNA sequencing
AU - Jacobson, K. Bruce
AU - Arlinghaus, Heinrich F.
AU - Buchanan, Michelle V.
AU - Chung-Hsuan Chen, Chen
AU - Glish, Gary L.
AU - Hettich, Robert L.
AU - McLuckey, Scott A.
PY - 1991/12
Y1 - 1991/12
N2 - The ability of the mass spectrometer to analyze collectively the masses of DNA fragments that are produced in the Sanger procedure for sequencing may allow the gel electrophoresis step to be eliminated. On the other hand, if gel electrophoresis is required, the use of resonance ionization spectroscopy coupled to a mass spectrometer may enable much faster analysis of DNA bands labeled with stable isotopes. Other combinations of labeling of the DNA and its mass spectrometric analysis with or without gel electrophoresis are also considered. Recent advances in these areas of mass spectrometry are reviewed.
AB - The ability of the mass spectrometer to analyze collectively the masses of DNA fragments that are produced in the Sanger procedure for sequencing may allow the gel electrophoresis step to be eliminated. On the other hand, if gel electrophoresis is required, the use of resonance ionization spectroscopy coupled to a mass spectrometer may enable much faster analysis of DNA bands labeled with stable isotopes. Other combinations of labeling of the DNA and its mass spectrometric analysis with or without gel electrophoresis are also considered. Recent advances in these areas of mass spectrometry are reviewed.
UR - http://www.scopus.com/inward/record.url?scp=44949271870&partnerID=8YFLogxK
U2 - 10.1016/1050-3862(91)90032-M
DO - 10.1016/1050-3862(91)90032-M
M3 - Article
C2 - 1817574
AN - SCOPUS:44949271870
SN - 1050-3862
VL - 8
SP - 223
EP - 229
JO - Genetic Analysis: Biomolecular Engineering
JF - Genetic Analysis: Biomolecular Engineering
IS - 8
ER -