Analysis of polymerase chain reaction-amplified dna products by mass spectrometry using matrix-assisted laser desorption and electrospray: Current status

M. J. Doktycz; G. B. Hurst; S. Habibigoudarzi; S. A. McLuckey; K. Tang; C. H. Chen; M. Uziel; K. B. Jacobson; R. P. Woychik; M. V. Buchanan

doi:10.1006/abio.1995.1465

Analysis of polymerase chain reaction-amplified dna products by mass spectrometry using matrix-assisted laser desorption and electrospray: Current status

M. J. Doktycz, G. B. Hurst, S. Habibigoudarzi, S. A. McLuckey, K. Tang, C. H. Chen, M. Uziel, K. B. Jacobson, R. P. Woychik, M. V. Buchanan

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Abstract

Recent advances in molecular biology are making it possible to diagnose genetic diseases and identify pathogens through the analysis of DNA. As clinical applications for molecular diagnosis increase, rapid, reliable methods for determination of DNA size will be needed. Mass spectrometry offers the potential of analyzing amplified DNA quickly and reliably, without the need for gel-based separation and sample labeling steps that are conventionally employed. Both electrospray ionization and matrix-assisted laser desorption/ionization have been evaluated for the size analysis of DNA using both synthetic oligonucleotides and PCR-amplified samples corresponding to bases 1626 to 1701 of the cystic fibrosis transmembrane conductance regulator gene. Both technologies have been demonstrated to have mass range and sensitivity required for the analysis of PCR-amplified DNA in this size range using minimal sample preparation. Steps required to incorporate either ionization technique into a reliable analytical scheme for the rapid, routine analysis of DNA are outlined.

Original language	English
Pages (from-to)	205-214
Number of pages	10
Journal	Analytical Biochemistry
Volume	230
Issue number	2
DOIs	https://doi.org/10.1006/abio.1995.1465
State	Published - Sep 1995

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Doktycz, M. J., Hurst, G. B., Habibigoudarzi, S., McLuckey, S. A., Tang, K., Chen, C. H., Uziel, M., Jacobson, K. B., Woychik, R. P., & Buchanan, M. V. (1995). Analysis of polymerase chain reaction-amplified dna products by mass spectrometry using matrix-assisted laser desorption and electrospray: Current status. Analytical Biochemistry, 230(2), 205-214. https://doi.org/10.1006/abio.1995.1465

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title = "Analysis of polymerase chain reaction-amplified dna products by mass spectrometry using matrix-assisted laser desorption and electrospray: Current status",

abstract = "Recent advances in molecular biology are making it possible to diagnose genetic diseases and identify pathogens through the analysis of DNA. As clinical applications for molecular diagnosis increase, rapid, reliable methods for determination of DNA size will be needed. Mass spectrometry offers the potential of analyzing amplified DNA quickly and reliably, without the need for gel-based separation and sample labeling steps that are conventionally employed. Both electrospray ionization and matrix-assisted laser desorption/ionization have been evaluated for the size analysis of DNA using both synthetic oligonucleotides and PCR-amplified samples corresponding to bases 1626 to 1701 of the cystic fibrosis transmembrane conductance regulator gene. Both technologies have been demonstrated to have mass range and sensitivity required for the analysis of PCR-amplified DNA in this size range using minimal sample preparation. Steps required to incorporate either ionization technique into a reliable analytical scheme for the rapid, routine analysis of DNA are outlined.",

author = "Doktycz, {M. J.} and Hurst, {G. B.} and S. Habibigoudarzi and McLuckey, {S. A.} and K. Tang and Chen, {C. H.} and M. Uziel and Jacobson, {K. B.} and Woychik, {R. P.} and Buchanan, {M. V.}",

year = "1995",

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doi = "10.1006/abio.1995.1465",

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Doktycz, MJ, Hurst, GB, Habibigoudarzi, S, McLuckey, SA, Tang, K, Chen, CH, Uziel, M, Jacobson, KB, Woychik, RP & Buchanan, MV 1995, 'Analysis of polymerase chain reaction-amplified dna products by mass spectrometry using matrix-assisted laser desorption and electrospray: Current status', Analytical Biochemistry, vol. 230, no. 2, pp. 205-214. https://doi.org/10.1006/abio.1995.1465

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T2 - Current status

AU - Doktycz, M. J.

AU - Hurst, G. B.

AU - Habibigoudarzi, S.

AU - McLuckey, S. A.

AU - Tang, K.

AU - Chen, C. H.

AU - Uziel, M.

AU - Jacobson, K. B.

AU - Woychik, R. P.

AU - Buchanan, M. V.

PY - 1995/9

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N2 - Recent advances in molecular biology are making it possible to diagnose genetic diseases and identify pathogens through the analysis of DNA. As clinical applications for molecular diagnosis increase, rapid, reliable methods for determination of DNA size will be needed. Mass spectrometry offers the potential of analyzing amplified DNA quickly and reliably, without the need for gel-based separation and sample labeling steps that are conventionally employed. Both electrospray ionization and matrix-assisted laser desorption/ionization have been evaluated for the size analysis of DNA using both synthetic oligonucleotides and PCR-amplified samples corresponding to bases 1626 to 1701 of the cystic fibrosis transmembrane conductance regulator gene. Both technologies have been demonstrated to have mass range and sensitivity required for the analysis of PCR-amplified DNA in this size range using minimal sample preparation. Steps required to incorporate either ionization technique into a reliable analytical scheme for the rapid, routine analysis of DNA are outlined.

AB - Recent advances in molecular biology are making it possible to diagnose genetic diseases and identify pathogens through the analysis of DNA. As clinical applications for molecular diagnosis increase, rapid, reliable methods for determination of DNA size will be needed. Mass spectrometry offers the potential of analyzing amplified DNA quickly and reliably, without the need for gel-based separation and sample labeling steps that are conventionally employed. Both electrospray ionization and matrix-assisted laser desorption/ionization have been evaluated for the size analysis of DNA using both synthetic oligonucleotides and PCR-amplified samples corresponding to bases 1626 to 1701 of the cystic fibrosis transmembrane conductance regulator gene. Both technologies have been demonstrated to have mass range and sensitivity required for the analysis of PCR-amplified DNA in this size range using minimal sample preparation. Steps required to incorporate either ionization technique into a reliable analytical scheme for the rapid, routine analysis of DNA are outlined.

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Analysis of polymerase chain reaction-amplified dna products by mass spectrometry using matrix-assisted laser desorption and electrospray: Current status

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