Abstract
The applications of fast atom bombardment mass spectrometry coupled with thin-layer chromatography in a study of phospholipids, alkaloids derived from bloodroot rhizomes, and diuretics are summarized. Use of the mass spectrometric data to trace compounds via characteristic fragment ions increases the effective resolution of the chromatographic resolution. Quantitative values derived in TLC/FAB experiments for the diuretic amiloride hydrochloride is linear over two orders of magnitude encompassing the clinically useful range. The use of a laser desorption ionization method and Fourier transform mass analysis for TLC plate detection is also described; matrix and concentration effects are significant in this case, but several successful applications are described. An interface device for the linkage.
| Original language | English |
|---|---|
| Pages (from-to) | 2841-2869 |
| Number of pages | 29 |
| Journal | Journal of Liquid Chromatography |
| Volume | 13 |
| Issue number | 14 |
| DOIs | |
| State | Published - Aug 1 1990 |
Funding
Acknowledgements J. C. Huffman and S. P. Horn of Indiana University provided initial guidance on the bloodroot alkaloids project. Jm is completing graduate research at Oak Ridge National Laboratory with the support of an Oak Ridge Associated Universities fellowship. The assistance and support of R. L. Hettich and M. V. Buchanan is gratefully acknowledged. We thank G. C. DiDonato for provision of the excat mass measurements and daughter ion MS/MS data in the study of alkaloids extracted from bloodroot.
| Funders | Funder number |
|---|---|
| Oak Ridge Associated Universities | |
| Oak Ridge National Laboratory |