Analysis of genetic diversity in banana cultivars (Musa cvs.) from the South of Oman using AFLP markers and classification by phylogenetic, hierarchical clustering and principal component analyses

Umezuruike Linus Opara, Dan Jacobson, Nadiya Abubakar Al-Saady

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40 Scopus citations

Abstract

Banana is an important crop grown in Oman and there is a dearth of information on its genetic diversity to assist in crop breeding and improvement programs. This study employed amplified fragment length polymorphism (AFLP) to investigate the genetic variation in local banana cultivars from the southern region of Oman. Using 12 primer combinations, a total of 1094 bands were scored, of which 1012 were polymorphic. Eighty-two unique markers were identified, which revealed the distinct separation of the seven cultivars. The results obtained show that AFLP can be used to differentiate the banana cultivars. Further classification by phylogenetic, hierarchical clustering and principal component analyses showed significant differences between the clusters found with molecular markers and those clusters created by previous studies using morphological analysis. Based on the analytical results, a consensus dendrogram of the banana cultivars is presented.

Original languageEnglish
Pages (from-to)332-341
Number of pages10
JournalJournal of Zhejiang University: Science B
Volume11
Issue number5
DOIs
StatePublished - May 2010
Externally publishedYes

Funding

* Project supported by Programs of Sultan Qaboos University (Nos. SR/AGR/BIOR/05/01 and IG/AGR/PLANT/04/01), Sultanate of Oman, and the Research Chair in Postharvest Technology at the University of Stellenbosch, South Africa © Zhejiang University and Springer-Verlag Berlin Heidelberg 2010

Keywords

  • Amplified fragment length polymorphism (AFLP)
  • Genetic diversity
  • Hierarchical clustering analysis (HCA)
  • Musa
  • Oman
  • Phylogenetics
  • Principal component analysis (PCA)

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