Analysis of Arabidopsis thaliana proteome by one- and two-dimensional chromatography coupled with mass spectrometry

Reno T. Nguyen; Theresa L. Savage; Nathan C. VerBerkmoes; Christine R. Schar; Robert L. Hettich; Barry D. Bruce

Analysis of Arabidopsis thaliana proteome by one- and two-dimensional chromatography coupled with mass spectrometry

Reno T. Nguyen, Theresa L. Savage, Nathan C. VerBerkmoes, Christine R. Schar, Robert L. Hettich, Barry D. Bruce

Bioanalytical Mass Spectrometry

Research output: Contribution to conference › Paper › peer-review

1 Scopus citations

Abstract

The analysis of the Arabidopsis thaliana proteome chloroplast fraction with LC-MS/MS techniques, was discussed. Arabidopsis thaliana plants were grown on MS agar with 1% sucrose for 20-30 days. The plants were harvested, chopped, and the cell walls digested with Cellulase and Macerozyme R-10. The chloroplast fraction was digested with trypsin and directly analyzed by 1D and 2D capillary/nano HPLC ES-MS/MS. Analysis of the chloroplast preparation by 1D HPLC coupled with MS resulted in the identification of 262 proteins with 1 unique peptide requirement and 106 proteins with 2 or more unique peptides per loci.

Original language	English
Pages	231-232
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	06/2/02 → 06/6/02

Cite this

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title = "Analysis of Arabidopsis thaliana proteome by one- and two-dimensional chromatography coupled with mass spectrometry",

abstract = "The analysis of the Arabidopsis thaliana proteome chloroplast fraction with LC-MS/MS techniques, was discussed. Arabidopsis thaliana plants were grown on MS agar with 1% sucrose for 20-30 days. The plants were harvested, chopped, and the cell walls digested with Cellulase and Macerozyme R-10. The chloroplast fraction was digested with trypsin and directly analyzed by 1D and 2D capillary/nano HPLC ES-MS/MS. Analysis of the chloroplast preparation by 1D HPLC coupled with MS resulted in the identification of 262 proteins with 1 unique peptide requirement and 106 proteins with 2 or more unique peptides per loci.",

author = "Nguyen, {Reno T.} and Savage, {Theresa L.} and VerBerkmoes, {Nathan C.} and Schar, {Christine R.} and Hettich, {Robert L.} and Bruce, {Barry D.}",

year = "2002",

language = "English",

pages = "231--232",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Analysis of Arabidopsis thaliana proteome by one- and two-dimensional chromatography coupled with mass spectrometry. / Nguyen, Reno T.; Savage, Theresa L.; VerBerkmoes, Nathan C. et al.
2002. 231-232 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Analysis of Arabidopsis thaliana proteome by one- and two-dimensional chromatography coupled with mass spectrometry

AU - Nguyen, Reno T.

AU - Savage, Theresa L.

AU - VerBerkmoes, Nathan C.

AU - Schar, Christine R.

AU - Hettich, Robert L.

AU - Bruce, Barry D.

PY - 2002

Y1 - 2002

N2 - The analysis of the Arabidopsis thaliana proteome chloroplast fraction with LC-MS/MS techniques, was discussed. Arabidopsis thaliana plants were grown on MS agar with 1% sucrose for 20-30 days. The plants were harvested, chopped, and the cell walls digested with Cellulase and Macerozyme R-10. The chloroplast fraction was digested with trypsin and directly analyzed by 1D and 2D capillary/nano HPLC ES-MS/MS. Analysis of the chloroplast preparation by 1D HPLC coupled with MS resulted in the identification of 262 proteins with 1 unique peptide requirement and 106 proteins with 2 or more unique peptides per loci.

AB - The analysis of the Arabidopsis thaliana proteome chloroplast fraction with LC-MS/MS techniques, was discussed. Arabidopsis thaliana plants were grown on MS agar with 1% sucrose for 20-30 days. The plants were harvested, chopped, and the cell walls digested with Cellulase and Macerozyme R-10. The chloroplast fraction was digested with trypsin and directly analyzed by 1D and 2D capillary/nano HPLC ES-MS/MS. Analysis of the chloroplast preparation by 1D HPLC coupled with MS resulted in the identification of 262 proteins with 1 unique peptide requirement and 106 proteins with 2 or more unique peptides per loci.

UR - http://www.scopus.com/inward/record.url?scp=0347422776&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:0347422776

SP - 231

EP - 232

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Analysis of Arabidopsis thaliana proteome by one- and two-dimensional chromatography coupled with mass spectrometry

Abstract

Conference

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