Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum

Gbekeloluwa B. Oguntimein; Miguel Rodriguez; Alexandru Dumitrache; Todd Shollenberger; Stephen R. Decker; Brian H. Davison; Steven D. Brown

doi:10.1007/s10529-017-2467-2

Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum

Gbekeloluwa B. Oguntimein, Miguel Rodriguez, Alexandru Dumitrache, Todd Shollenberger, Stephen R. Decker, Brian H. Davison, Steven D. Brown

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Objective: To develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential. Results: Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain. Conclusion: A prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.

Original language	English
Pages (from-to)	303-308
Number of pages	6
Journal	Biotechnology Letters
Volume	40
Issue number	2
DOIs	https://doi.org/10.1007/s10529-017-2467-2
State	Published - Feb 1 2018

Funding

Acknowledgements We thank Thermo Fisher Scientific for supplying several test plates. This research was funded by the BioEnergy Science Center (BESC), which is a US Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. GBO was supported by the U.S. Department of Energy, Office of Science, and Office of Workforce Development for Teachers and Scientists (WDTS) under the Visiting Faculty Program (VFP). ORNL is managed by UT-Battelle, LLC, Oak Ridge, TN, USA, for the DOE under contract DE-AC05-00OR22725.

Funders	Funder number
BioEnergy Science Center
DOE Office of Science
Office of Biological and Environmental Research
US Department of Energy Bioenergy Research Center
U.S. Department of Energy	DE-AC05-00OR22725
Office of Science
Workforce Development for Teachers and Scientists
Oak Ridge National Laboratory

Keywords

Bioethanol
Consolidated bioprocessing
High throughput screening
Lignocellulosic biomass
Microplate assay

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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abstract = "Objective: To develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential. Results: Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain. Conclusion: A prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.",

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AU - Davison, Brian H.

AU - Brown, Steven D.

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N2 - Objective: To develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential. Results: Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain. Conclusion: A prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.

AB - Objective: To develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential. Results: Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain. Conclusion: A prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.

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Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum

Abstract

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Keywords

UN SDGs

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