TY - JOUR
T1 - Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum
AU - Oguntimein, Gbekeloluwa B.
AU - Rodriguez, Miguel
AU - Dumitrache, Alexandru
AU - Shollenberger, Todd
AU - Decker, Stephen R.
AU - Davison, Brian H.
AU - Brown, Steven D.
N1 - Publisher Copyright:
© 2017, The Author(s).
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Objective: To develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential. Results: Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain. Conclusion: A prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.
AB - Objective: To develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential. Results: Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain. Conclusion: A prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.
KW - Bioethanol
KW - Consolidated bioprocessing
KW - High throughput screening
KW - Lignocellulosic biomass
KW - Microplate assay
UR - http://www.scopus.com/inward/record.url?scp=85033449230&partnerID=8YFLogxK
U2 - 10.1007/s10529-017-2467-2
DO - 10.1007/s10529-017-2467-2
M3 - Article
C2 - 29124514
AN - SCOPUS:85033449230
SN - 0141-5492
VL - 40
SP - 303
EP - 308
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 2
ER -
