Abstract
Objective: To develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential. Results: Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain. Conclusion: A prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.
| Original language | English |
|---|---|
| Pages (from-to) | 303-308 |
| Number of pages | 6 |
| Journal | Biotechnology Letters |
| Volume | 40 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2018 |
Funding
Acknowledgements We thank Thermo Fisher Scientific for supplying several test plates. This research was funded by the BioEnergy Science Center (BESC), which is a US Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. GBO was supported by the U.S. Department of Energy, Office of Science, and Office of Workforce Development for Teachers and Scientists (WDTS) under the Visiting Faculty Program (VFP). ORNL is managed by UT-Battelle, LLC, Oak Ridge, TN, USA, for the DOE under contract DE-AC05-00OR22725.
Keywords
- Bioethanol
- Consolidated bioprocessing
- High throughput screening
- Lignocellulosic biomass
- Microplate assay