An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation

Mónica Prías-Blanco; Timothy M. Chappell; Emily F. Freed; Eudald Illa-Berenguer; Carrie A. Eckert; Wayne A. Parrott

doi:10.1007/s11248-022-00328-4

An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation

Mónica Prías-Blanco, Timothy M. Chappell, Emily F. Freed, Eudald Illa-Berenguer, Carrie A. Eckert, Wayne A. Parrott

Synthetic Biology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Auxotrophic strains of Agrobacterium tumefaciens can contribute to the development of more efficient transformation systems, especially for crops historically considered recalcitrant. Homologous recombination was used to derive methionine auxotrophs of two common A. tumefaciens strains, LBA4404 and EHA105. The EHA105 strains were more efficient for switchgrass transformation, while both the EHA105 and LBA4404 strains worked equally well for the rice control. Event quality, as measured by transgene copy number, was not affected by auxotrophy, but was higher for the LBA4404 strains than the EHA105 strains. Ultimately, the use of auxotrophs reduced bacterial overgrowth during co-cultivation and decreased the need for antibiotics.

Original language	English
Pages (from-to)	661-676
Number of pages	16
Journal	Transgenic Research
Volume	31
Issue number	6
DOIs	https://doi.org/10.1007/s11248-022-00328-4
State	Published - Dec 2022

Funding

Funding was provided by The Center for Bioenergy Innovation, a U.S. Department of Energy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. The assistance of Henry Vencill and Christian Ona with tissue transfers is gratefully acknowledged. Funding was provided by The Center for Bioenergy Innovation, a U.S. Department of Energy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science.

Funders	Funder number
U.S. Department of Energy Research Center
Office of Science
Biological and Environmental Research
Center for Bioenergy Innovation

Keywords

Agrobacterium tumefaciens
Auxotrophy
Rice
Switchgrass
Transformation

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10.1007/s11248-022-00328-4

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title = "An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation",

abstract = "Auxotrophic strains of Agrobacterium tumefaciens can contribute to the development of more efficient transformation systems, especially for crops historically considered recalcitrant. Homologous recombination was used to derive methionine auxotrophs of two common A. tumefaciens strains, LBA4404 and EHA105. The EHA105 strains were more efficient for switchgrass transformation, while both the EHA105 and LBA4404 strains worked equally well for the rice control. Event quality, as measured by transgene copy number, was not affected by auxotrophy, but was higher for the LBA4404 strains than the EHA105 strains. Ultimately, the use of auxotrophs reduced bacterial overgrowth during co-cultivation and decreased the need for antibiotics.",

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AU - Prías-Blanco, Mónica

AU - Chappell, Timothy M.

AU - Freed, Emily F.

AU - Illa-Berenguer, Eudald

AU - Eckert, Carrie A.

AU - Parrott, Wayne A.

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AB - Auxotrophic strains of Agrobacterium tumefaciens can contribute to the development of more efficient transformation systems, especially for crops historically considered recalcitrant. Homologous recombination was used to derive methionine auxotrophs of two common A. tumefaciens strains, LBA4404 and EHA105. The EHA105 strains were more efficient for switchgrass transformation, while both the EHA105 and LBA4404 strains worked equally well for the rice control. Event quality, as measured by transgene copy number, was not affected by auxotrophy, but was higher for the LBA4404 strains than the EHA105 strains. Ultimately, the use of auxotrophs reduced bacterial overgrowth during co-cultivation and decreased the need for antibiotics.

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KW - Switchgrass

KW - Transformation

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An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation

Abstract

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Keywords

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