Abstract
Autografting is currently the gold standard for treatment of bone defects, but has shown disadvantages in the limited volume of and donor site morbidity associated with harvested bone. Customized bone scaffolds that mimic the mechanical and biological properties of native bone are needed to augment the currently limited bone regeneration strategies. To achieve this goal, a repeated cross-hatch structure with uniform cubic pores was designed and 3D printed using polylactic acid (PLA) via fused deposition modeling (FDM). PLA surfaces were modified by wet chemical (alkali) treatment for either 1 h (1hAT) or 6 h (6hAT), followed by coating with nano-hydroxyapatite (nHA). Our hypotheses were that: (i) 6-hour (but not 1-hour) alkali treatment would enhance nHA coating, (ii) the nHA coating on the 6-hour alkali-treated surface would increase hydrophilicity and cell attachment/proliferation, and (iii) stiffness, but not effective Young's modulus, would be reduced by 6-hour alkali treatment. The effects of AT and nHA coating on scaffold morphology was observed by scanning electron microscopy and quantified using a custom MATLAB script. Chemical composition and hydrophilicity were evaluated via energy dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy, and water contact angle analyses, respectively. Mechanical testing and in vitro cell culture were further employed to analyze compressive properties, and cell attachment and proliferation, respectively. As expected, 6hAT led to reduced strut width and stiffness, while improving the nHA coating and hydrophilicity. Interestingly, PLA/6hAT but not PLA/6hAT/nHA demonstrated a reduction in effective modulus compared to PLA and PLA/nHA scaffolds. From in vitro experiments, the combined PLA/6hAT/nHA modification resulted in the greatest extent of cell attachment but not proliferation. These results collectively demonstrate that the PLA/6hAT/nHA scaffold exhibits properties that may prove beneficial for cancellous bone regeneration.
| Original language | English |
|---|---|
| Article number | 111686 |
| Journal | Materials Science and Engineering C |
| Volume | 120 |
| DOIs | |
| State | Published - Jan 2021 |
| Externally published | Yes |
Funding
Funding for this work was provided by Mississippi State University's Bagley College of Engineering , Office of Research and Economic Development , and Institute for Imaging and Analytical Technologies . Thanks to Stephen Horstemeyer at the Center for Advanced Vehicular Systems (CAVS) for assistance with SEM-EDX analyses. Funding for this work was provided by Mississippi State University's Bagley College of Engineering, Office of Research and Economic Development, and Institute for Imaging and Analytical Technologies. Thanks to Stephen Horstemeyer at the Center for Advanced Vehicular Systems (CAVS) for assistance with SEM-EDX analyses.
Keywords
- 3D printing
- Alkali treatment
- Mechanical properties
- Nano-hydroxyapatite
- Polylactic acid scaffold
- Surface functionalisation