Alert-type biological dosimeter based on enzyme logic system

Vera Bocharova; Jan Halámek; Jian Zhou; Guinevere Strack; Joseph Wang; Evgeny Katz

doi:10.1016/j.talanta.2011.03.034

Alert-type biological dosimeter based on enzyme logic system

Vera Bocharova, Jan Halámek, Jian Zhou, Guinevere Strack, Joseph Wang, Evgeny Katz

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A cooperative effect of two biomarkers, α-amylase and lactate dehydrogenase, was used to analyze radiation-caused tissue damage in vitro in model solutions of human serum. The analytical system was based on the recently emerged biocomputing concept applying biocatalytic cascades for logic processing of biochemical input signals. The studied system resembled a Boolean NAND logic gate in which the change of the optical output signal from a high level (logic value 1) to a low level (logic value 0) confirmed the presence of both biomarkers at pathological concentrations (1,1 input signals), thus yielding the conclusion about radiation tissue damage. The system operates in a digital YES/NO format as an alert-type biosensor with a built-in Boolean logic.

Original language	English
Pages (from-to)	800-803
Number of pages	4
Journal	Talanta
Volume	85
Issue number	1
DOIs	https://doi.org/10.1016/j.talanta.2011.03.034
State	Published - Jul 15 2011
Externally published	Yes

Funding

This work was supported by the Office of Naval Research in the frame of the project: “Integrated Enzyme-Logic Systems for “Sense and Treat” Injured Soldiers” (Award #N00014-08-1-1202).

Funders	Funder number
Office of Naval Research	00014-08-1-1202

Keywords

Biocomputing
Biological dosimeter
Biomarker
Digital biosensor
Enzyme logic

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10.1016/j.talanta.2011.03.034

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title = "Alert-type biological dosimeter based on enzyme logic system",

abstract = "A cooperative effect of two biomarkers, α-amylase and lactate dehydrogenase, was used to analyze radiation-caused tissue damage in vitro in model solutions of human serum. The analytical system was based on the recently emerged biocomputing concept applying biocatalytic cascades for logic processing of biochemical input signals. The studied system resembled a Boolean NAND logic gate in which the change of the optical output signal from a high level (logic value 1) to a low level (logic value 0) confirmed the presence of both biomarkers at pathological concentrations (1,1 input signals), thus yielding the conclusion about radiation tissue damage. The system operates in a digital YES/NO format as an alert-type biosensor with a built-in Boolean logic.",

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AU - Bocharova, Vera

AU - Halámek, Jan

AU - Zhou, Jian

AU - Strack, Guinevere

AU - Wang, Joseph

AU - Katz, Evgeny

PY - 2011/7/15

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AB - A cooperative effect of two biomarkers, α-amylase and lactate dehydrogenase, was used to analyze radiation-caused tissue damage in vitro in model solutions of human serum. The analytical system was based on the recently emerged biocomputing concept applying biocatalytic cascades for logic processing of biochemical input signals. The studied system resembled a Boolean NAND logic gate in which the change of the optical output signal from a high level (logic value 1) to a low level (logic value 0) confirmed the presence of both biomarkers at pathological concentrations (1,1 input signals), thus yielding the conclusion about radiation tissue damage. The system operates in a digital YES/NO format as an alert-type biosensor with a built-in Boolean logic.

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Alert-type biological dosimeter based on enzyme logic system

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