Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research

Degao Liu; Rongbin Hu; Kaitlin J. Palla; Gerald A. Tuskan; Xiaohan Yang

doi:10.1016/j.pbi.2016.01.007

Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research

Degao Liu, Rongbin Hu, Kaitlin J. Palla, Gerald A. Tuskan, Xiaohan Yang

Research output: Contribution to journal › Review article › peer-review

93 Scopus citations

Abstract

Genome editing with site-specific nucleases has become a powerful tool for functional characterization of plant genes and genetic improvement of agricultural crops. Among the various site-specific nuclease-based technologies available for genome editing, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems have shown the greatest potential for rapid and efficient editing of genomes in plant species. This article reviews the current status of application of CRISPR/Cas9 to plant genomics research, with a focus on loss-of-function and gain-of-function analysis of individual genes in the context of perennial plants and the potential application of CRISPR/Cas9 to perturbation of gene expression, and identification and analysis of gene modules as part of an accelerated domestication and synthetic biology effort.

Original language	English
Pages (from-to)	70-77
Number of pages	8
Journal	Current Opinion in Plant Biology
Volume	30
DOIs	https://doi.org/10.1016/j.pbi.2016.01.007
State	Published - Apr 1 2016

Funding

This research is supported by the Department of Energy (DOE), Office of Science, Genomic Science Program under Award Number DE-SC0008834 . The authors would like to thank Lee E. Gunter for critical review and comments on the manuscript. Oak Ridge National Laboratory is managed by UT-Battelle, LLC for the US DOE under Contract Number DE-AC05-00OR22725.

Funders	Funder number
U.S. Department of Energy	DE-AC05-00OR22725
Office of Science	DE-SC0008834
Oak Ridge National Laboratory

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.pbi.2016.01.007

Cite this

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abstract = "Genome editing with site-specific nucleases has become a powerful tool for functional characterization of plant genes and genetic improvement of agricultural crops. Among the various site-specific nuclease-based technologies available for genome editing, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems have shown the greatest potential for rapid and efficient editing of genomes in plant species. This article reviews the current status of application of CRISPR/Cas9 to plant genomics research, with a focus on loss-of-function and gain-of-function analysis of individual genes in the context of perennial plants and the potential application of CRISPR/Cas9 to perturbation of gene expression, and identification and analysis of gene modules as part of an accelerated domestication and synthetic biology effort.",

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Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research

Abstract

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