Advances and application of CRISPR-Cas systems

Rongming Liu; Liya Liang; Margaret Habib; Emily F. Freed; Carrie A. Eckert

doi:10.1016/B978-0-12-824469-2.00029-4

Advances and application of CRISPR-Cas systems

Rongming Liu, Liya Liang, Margaret Habib, Emily F. Freed, Carrie A. Eckert

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

A new gene-editing technique called clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins have revolutionized genome engineering because of its high efficiency, relatively low cost, and ease of use compared with other techniques such as zinc-finger nucleases and transcription activator-like effector nucleases. CRISPR-Cas systems have transformed biological research, quickly becoming the preferred method for engineering specific genome sequences in industrially relevant microbes, important food crops, and human cells. This powerful new tool has the potential for limitless applications ranging from the treatment of human diseases to improving food security to the generation of renewable and sustainable bioproducts and biofuels. In this chapter, we will discuss advances and applications for these CRISPR-Cas systems in biological engineering.

Original language	English
Title of host publication	New Frontiers and Applications of Synthetic Biology
Publisher	Elsevier
Pages	331-348
Number of pages	18
ISBN (Electronic)	9780128244692
DOIs	https://doi.org/10.1016/B978-0-12-824469-2.00029-4
State	Published - Jan 1 2022
Externally published	Yes

Keywords

Bacterial
CRISPR-Cas
Gene regulation
Genome editing
Human cells
Plant
Yeast

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10.1016/B978-0-12-824469-2.00029-4

Cite this

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AU - Liang, Liya

AU - Habib, Margaret

AU - Freed, Emily F.

AU - Eckert, Carrie A.

PY - 2022/1/1

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AB - A new gene-editing technique called clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins have revolutionized genome engineering because of its high efficiency, relatively low cost, and ease of use compared with other techniques such as zinc-finger nucleases and transcription activator-like effector nucleases. CRISPR-Cas systems have transformed biological research, quickly becoming the preferred method for engineering specific genome sequences in industrially relevant microbes, important food crops, and human cells. This powerful new tool has the potential for limitless applications ranging from the treatment of human diseases to improving food security to the generation of renewable and sustainable bioproducts and biofuels. In this chapter, we will discuss advances and applications for these CRISPR-Cas systems in biological engineering.

KW - Bacterial

KW - CRISPR-Cas

KW - Gene regulation

KW - Genome editing

KW - Human cells

KW - Plant

KW - Yeast

UR - https://www.scopus.com/pages/publications/85128578978

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BT - New Frontiers and Applications of Synthetic Biology

PB - Elsevier

ER -

Advances and application of CRISPR-Cas systems

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Keywords

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