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Adsorption of cellobiohydrolases I onto lignin fractions from dilute acid pretreated Broussonetia papyrifera

  • Lan Yao
  • , Haitao Yang
  • , Chang Geun Yoo
  • , Xianzhi Meng
  • , Mi Li
  • , Yunqiao Pu
  • , Arthur J. Ragauskas
  • , Robert W. Sykes

    Research output: Contribution to journalArticlepeer-review

    35 Scopus citations

    Abstract

    Broussonetia papyrifera, known as paper mulberry, is a potential feed stock for bioethanol production because of its cellulose-rich composition. Lignin in dilute acid pretreated Broussonetia papyrifera was fractionated to three different fractions, and their physiochemical properties were determined by FT-IR, GPC and NMR analyses. Different structural characteristics were observed from each lignin fraction. Cellobiohydrolases I (CBH) adsorption to each lignin was understood by the lignin properties. The results showed that aliphatic hydroxyl groups in lignin showed positive correlations with the maximum binding ability of CBH onto lignin samples. Also, the contents of phenolic compounds such as p-hydroxyphenyl benzoate (PB), syringyl (S) and guaiacyl (G) units in the lignin influenced their CBH binding.

    Original languageEnglish
    Pages (from-to)957-962
    Number of pages6
    JournalBioresource Technology
    Volume244
    DOIs
    StatePublished - Nov 2017

    Funding

    The authors are grateful for the support by National Natural Science Foundation of China (No. 31500496 ), China Scholarship Council (NO. 2011842330 and NO. 201508420257 ) and Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education of China (NO. KF-201409 and NO. KF-201611 ), key project of Hubei Provincial Department of Education (NO. D20161402) and Foundation (No. 201611B01) of Hubei Provincial Key Laboratory of Green Materials for Light Industry.

    Keywords

    • Adsorption
    • Broussonetia papyrifera
    • Cellobiohydrolases I
    • Dilute acid pretreatment
    • Lignin fraction

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