Abstract
About 160 species are classified within the Viburnum genus and many of these are cultivated for horticultural purposes. The vast dispersal of Viburnum makes the genus a useful model for studying evolutionary history and inferring how species expanded into their current distributions. Simple sequence repeat (SSR) markers were previously developed for five Viburnum species that were classified within the four major clades (Laminotinus, Crenotinus, Valvatotinus, and Porphyrotinus). The ability of some of these markers to cross-amplify in Viburnum species has been scantly evaluated, but there has not been any genus-wide assessment for the markers. We evaluated a collection of 49 SSR markers for the ability to cross-amplify in 224 samples, including 46 Viburnum species, representing all 16 subclades, and five additional species in the Viburnaceae and Caprifoliaceae. A subset of 14 potentially comprehensive markers for Viburnum species was identified and evaluated for the ability to detect polymorphisms in species outside of their respective clades. The 49 markers had overall amplification success in 52% of the samples, including a 60% success rate within the Viburnum genus and 14% in other genera. The comprehensive marker set amplified alleles in 74% of all samples tested, including 85% of Viburnum samples and 19% of outgroup samples. To the best of our knowledge, this is the first comprehensive set of markers able to characterize species across an entire genus. This set of markers can be used to assess the genetic diversity and population structure of most Viburnum species and closely allied species.
| Original language | English |
|---|---|
| Article number | 5343 |
| Journal | Scientific Reports |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 2023 |
Funding
The authors gratefully recognize the U.S. National Arboretum Herbarium, The Morton Arboretum, U.S. National Arboretum, Yale Peabody Museum, Herbarium of the Arnold Arboretum, Arnold Arboretum, Spring Grove Cemetery and Arboretum, Mt. Airy Arboretum, JC Raulston Arboretum, Morris Arboretum, University of Tennessee Herbarium, Carnegie Museum of Natural History Herbarium, University of Washington Botanical Garden, and A.C. Moore Herbarium for the donation of plant material for this study. We also thank Shade Niece for his technical help. This work was supported by a Non-Assistance Cooperative Agreement between the University of Tennessee and USDA, ARS (NACA 58-6062-6). The authors gratefully recognize the U.S. National Arboretum Herbarium, The Morton Arboretum, U.S. National Arboretum, Yale Peabody Museum, Herbarium of the Arnold Arboretum, Arnold Arboretum, Spring Grove Cemetery and Arboretum, Mt. Airy Arboretum, JC Raulston Arboretum, Morris Arboretum, University of Tennessee Herbarium, Carnegie Museum of Natural History Herbarium, University of Washington Botanical Garden, and A.C. Moore Herbarium for the donation of plant material for this study. We also thank Shade Niece for his technical help. This work was supported by a Non-Assistance Cooperative Agreement between the University of Tennessee and USDA, ARS (NACA 58-6062-6).