Abstract
An efficient receptor-mediated non-viral gene delivery formulation based on mono-pegylated recombinant human epidermal growth factor (EGF) was developed using a streptavidin-biotin system. Biotin-derivatized and mono-pegylated EGF was prepared by conjugating a biotin-PEG-NHS derivative to EGF and purified through a chromatographic method. Luciferase plasmid DNA and polyethylenimine (PEI) were complexed to form positively charged nanoparticles on which negatively charged streptavidin was first coated and then biotin-PEG-EGF conjugate was immobilized via streptavidin-biotin interaction. The EGF-PEG-biotin-streptavidin-PEI-DNA complexes were characterized in terms of their effective diameter and surface zeta (ζ)-potential value under various formulation conditions. The formulated complexes exhibited high transfection efficiency (∼108 in luciferase activity) with no inter-particle aggregation. This was attributed to enhanced cellular uptake of the resultant complexes via receptor-mediated endocytosis. Furthermore, in the presence of serum proteins, a slight decrease in transfection efficiency was observed due to the presence of PEG chains on the surface.
| Original language | English |
|---|---|
| Pages (from-to) | 109-119 |
| Number of pages | 11 |
| Journal | Journal of Controlled Release |
| Volume | 83 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 18 2002 |
| Externally published | Yes |
Funding
This work was supported by the Ministry of Health and Welfare, South Korea (Grant No. HMP-99-B-02-0002).
| Funders | Funder number |
|---|---|
| Ministry of Health and Welfare | HMP-99-B-02-0002 |
Keywords
- EGF
- Polyethylene glycol (PEG)
- Receptor-mediated gene delivery
- Streptavidin-biotin