TY - JOUR
T1 - A rare gain of function mutation in a wheat tandem kinase confers resistance to powdery mildew
AU - Lu, Ping
AU - Guo, Li
AU - Wang, Zhenzhong
AU - Li, Beibei
AU - Li, Jing
AU - Li, Yahui
AU - Qiu, Dan
AU - Shi, Wenqi
AU - Yang, Lijun
AU - Wang, Ning
AU - Guo, Guanghao
AU - Xie, Jingzhong
AU - Wu, Qiuhong
AU - Chen, Yongxing
AU - Li, Miaomiao
AU - Zhang, Huaizhi
AU - Dong, Lingli
AU - Zhang, Panpan
AU - Zhu, Keyu
AU - Yu, Dazhao
AU - Zhang, Yan
AU - Deal, Karin R.
AU - Huo, Naxin
AU - Liu, Cuimin
AU - Luo, Ming Cheng
AU - Dvorak, Jan
AU - Gu, Yong Qiang
AU - Li, Hongjie
AU - Liu, Zhiyong
N1 - Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive diseases that pose a great threat to wheat production. Wheat landraces represent a rich source of powdery mildew resistance. Here, we report the map-based cloning of powdery mildew resistance gene Pm24 from Chinese wheat landrace Hulutou. It encodes a tandem kinase protein (TKP) with putative kinase-pseudokinase domains, designated WHEAT TANDEM KINASE 3 (WTK3). The resistance function of Pm24 was validated by transgenic assay, independent mutants, and allelic association analyses. Haplotype analysis revealed that a rare 6-bp natural deletion of lysine-glycine codons, endemic to wheat landraces of Shaanxi Province, China, in the kinase I domain (Kin I) of WTK3 is critical for the resistance function. Transgenic assay of WTK3 chimeric variants revealed that only the specific two amino acid deletion, rather than any of the single or more amino acid deletions, in the Kin I of WTK3 is responsible for gaining the resistance function of WTK3 against the Bgt fungus.
AB - Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive diseases that pose a great threat to wheat production. Wheat landraces represent a rich source of powdery mildew resistance. Here, we report the map-based cloning of powdery mildew resistance gene Pm24 from Chinese wheat landrace Hulutou. It encodes a tandem kinase protein (TKP) with putative kinase-pseudokinase domains, designated WHEAT TANDEM KINASE 3 (WTK3). The resistance function of Pm24 was validated by transgenic assay, independent mutants, and allelic association analyses. Haplotype analysis revealed that a rare 6-bp natural deletion of lysine-glycine codons, endemic to wheat landraces of Shaanxi Province, China, in the kinase I domain (Kin I) of WTK3 is critical for the resistance function. Transgenic assay of WTK3 chimeric variants revealed that only the specific two amino acid deletion, rather than any of the single or more amino acid deletions, in the Kin I of WTK3 is responsible for gaining the resistance function of WTK3 against the Bgt fungus.
UR - http://www.scopus.com/inward/record.url?scp=85078925015&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-14294-0
DO - 10.1038/s41467-020-14294-0
M3 - Article
C2 - 32015344
AN - SCOPUS:85078925015
SN - 2041-1723
VL - 11
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 680
ER -